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Serbian Journal of Experimental and Clinical Research
2014, vol. 15, br. 3, str. 115-120
jezik rada: engleski
vrsta rada: rad po pozivu
doi:10.5937/sjecr1403115M


Hormon-produkujuće ćelije hipofize nakon primene estradiola u animalnom modelu menopauze
Univerzitet u Beogradu, Institut za biološka istraživanja 'Siniša Stanković', Beograd

e-adresa: dimi@ibiss.bg.ac.rs

Projekat

Odgovor neuroendokrinog sistema pacova na odabrane biljne ekstrakte, fitoestrogene, steroidne i peptidne hormone (MPNTR - 173009)

Sažetak

Starenje kod ženskog pola je biološki proces tokom kojeg dolazi do promena u strukturi i funkciji endokrinih ćelija i tkiva, a posebnu osetljivost ispoljavaju hipofizne hormonprodukujuće ćelije. Pored oslobađajućih hormona hipotalamusa i estradiol ostvaruje zapaženu ulogu u regulaciji sinteze i sekrecije hipofiznih hormona, a još uvek se koristi u terapiji menopauzalnih tegoba. Efekti starenja ili ovarijektomije, kao i primene sintetskog estradiola pod tim okolnostima su ispitivani na hormon-produkujućim ćelijama hipofize ženki pacova srednjeg doba (animalni modeli menopauze) i to: gonadotropima (FSH i LH), tirotropima (TSH), somatotropima (GH), mamotropima (PRL) i kortikotropima (ACTH). Sve ćelije su imunohistohemijski bojene i histološki analizirane, dok je za određivanje nivoa hormona u krvi korišćena ELISA metoda. Utvrđeno je da starenje prouzrokuje različite, uglavnom reduktivne promene volumena, broja i sekrecije hormon-produkujućih ćelija hipofize u našim animalnim modelima menopauze, izuzev na primeru PRL ćelija čiji je broj značajno povećan, a sekrecija intenzivirana. Posle tretmana ženki pacova srednjeg doba estradiolom apsolutna i relativna masa hipofize je značajno povećana u poređenju sa kontrolnim ženkama. Histološki parametri poput volumena ćelija i volumenske gustine PRL i ACTH ćelija su značajno povećani u poređenju sa kontrolnim vrednostima. Sa druge strane, pomenuti parametri u FSH, LH i GH, a u izvesnim slučajevima i TSH ćelijama su značajno smanjeni nakon tretmana estradiolom, u poređenju sa kontrolama. Koncentracije odgovarajućih hormona u krvi su pratile navedene promene histoloških parametara. Ova zapažanja ukazuju da estradiol davan menopauzalnim jedinkama ženskog pola može na specifičan način, dvosmerno, modifikovati histološke karakteristike i sekretornu aktivnost različitih hormon-produkujućih ćelija hipofize.

Ključne reči

ženke pacova; srednje doba; hipofizne ćelije; estradiol

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