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Food and Feed Research
2017, vol. 44, br. 1, str. 1-10
jezik rada: engleski
vrsta rada: originalan članak
doi:10.5937/FFR1701001C

Creative Commons License 4.0
Optimizacija sinteze galakto-oligosaharida pomoću metoda odzivnih površina
Tehnološko-metalurški fakultet, Katedra za biohemijsko inženjerstvo i biotehnologiju, Beograd

e-adresa: mcarevic@tmf.bg.ac.rs

Projekat

Razvoj novih inkapsulacionih i enzimskih tehnologija za proizvodnju biokatalizatora i biološki aktivnih komponenata hrane u cilju povecanja njene konkurentnosti, kvaliteta i bezbednosti (MPNTR - 46010)

Sažetak

Galakto-oligosaharidi (GOS) obuhvataju raznorodnu grupu nesvarljivih ugljenih hidrata, derivata laktoze, koji se smatraju prebioticima zahvaljujući brojnim naučnim studijama u kojima su dokazana njihova izuzetna fizičko-hemijska, kao i fiziološka svojstva. Ova saznanja omogućila su njihovu široku primenu u prehrambenoj i farmaceutskoj industriji, kao i u industriji stočne hrane. Galakto-oligosaharidi se skoro isključivo proizvode enzimskom sintezom pomoću enzima β-galaktozidaze. Međutim, poznato je da ovaj enzim istovremeno obavlja i svoju osnovnu biološku funkciju, a to je hidroliza laktoze, pa je od presudnog značaja steći uvid u uticaj različitih reakcionih faktora, i na taj način omogućiti favorizovanje reakcije transgalaktozilacije, odnosno sinteze galakto-oligosaharida. U ovoj studiji, u cilju ispitivanja uticaja pojedinačnih reakcionih para-metara, uočavanja i razumevanja njihovih međusobnih efekata i na kraju određivanja optimalnih uslova za povećan prinos galakto-oligosaharida, primenjeni su metodi statističkog planiranja eksperimenata i metod odzivnih površina (RSM). Konačno, nakon analize rezultata primećeno je da temperatura i rN nemaju značajan uticaj na prinos galakto-oligosaharida, dok su sa druge strane optimalne vrednosti signifikantnih faktora: koncentracija laktoze 400 g/l, koncentracija enzima 13,5 g/l i vreme 13 minuta.

Ključne reči

galakto-oligosaharidi; β-galaktozidaza; Aspergillus oryzae; optimizacija; metod odzivnih površina

Reference

Arreola, S.L., Intanon, M., Pham, N.H., Haltrich, D., Nguyen, T.H. (2014) Galacto-oligosaccharides recent progress on research and application as prebiotics. u: Galactose: Structure and Function in Biology and Medicine, New York: Nova Science Publishers, Inc, pp. 75-105
Bruno-Barcena, J.M., Azcarate-Peril, M. A. (2015) Galacto-oligosaccharides and colorectal cancer: Feeding our intestinal probiome. Journal of Functional Foods, 12: 92-108
Carević, M., Bezbradica, D., Banjanac, K., Milivojević, A., Fanuel, M., Rogniaux, H., Ropartz, D., Veličković, D. (2016) Structural Elucidation of Enzymatically Synthesized Galacto-oligosaccharides Using Ion-Mobility Spectrometry-Tandem Mass Spectrometry. Journal of Agricultural and Food Chemistry, 64(18): 3609-3615
Carević, M., Ćorović, M., Mihailović, M., Banjanac, K., Milisavljević, A., Veličković, D., Bezbradica, D. (2016) Galacto-oligosaccharide synthesis using chemically modified β-galactosidase from Aspergillus oryzae immobilised onto macroporous amino resin. International Dairy Journal, 54: 50-57
Chen, X.Y., Gänzle, M.G. (2017) Lactose and lactose-derived oligosaccharides: More than prebiotics?. International Dairy Journal, 67: 61-72
Geiger, B., Nguyen, H., Wenig, S., Nguyen, H.A., Lorenz, C., Kittl, R., Mathiesen, G., Eijsink, V.G.H., Haltrich, D., Nguyen, T. (2016) From by-product to valuable components: Efficient enzymatic conversion of lactose in whey using β-galactosidase from Streptococcus thermophilus. Biochemical Engineering Journal, 116: 45-53
Gosling, A., Stevens, G.W., Barber, A.R., Kentish, S.E., Gras, S.L. (2010) Recent advances refining galactooligosaccharide production from lactose. Food Chemistry, 121(2): 307-318
Hong, K.B., Kim, J.H., Kwon, H.K., Han, S.H., Park, Y., Suh, H.J. (2016) Evaluation of prebiotic effects of high-purity galactooligo-saccharides in vitro and in vivo. Food Technology and Biotechnology, 156-163; 54
Huerta, L.M., Vera, C., Guerrero, C., Wilson, L., Illanes, A. (2011) Synthesis of galacto-oligosaccharides at very high lactose concentrations with immobilized β-galactosidases from Aspergillus oryzae. Process Biochemistry, 46(1): 245-252
Lisboa, C.R., de Simoni, M.L., Trindade, R.A., de Almeida, C.F.A., de Medeiros, B.J.F., Burkert, C.A.V. (2012) Response surface methodology applied to the enzymatic synthesis of galacto-oligosaccharides from cheese whey. Food Science and Biotechnology, 21(6): 1519-1524
Macfarlane, G.T., Steed, H., Macfarlane, S. (2008) Bacterial metabolism and health related effects of galacto-oligosaccharides and other prebiotics. Journal of Applied Microbiology, 305-344; 104
Mahoney, R.R. (1998) Galactosyl-oligosaccharide formation during lactose hydrolysis: A review. Food Chemistry, 63(2): 147-154
Milisavljević, A., Stojanović, M., Carević, M., Mihailović, M., Veličković, D., Milosavić, N., Bezbradica, D. (2014) Lipase-Catalyzed Esterification of Phloridzin: Acyl Donor Effect on Enzymatic Affinity and Antioxidant Properties of Esters. Industrial & Engineering Chemistry Research, 53(43): 16644-16651
Otieno, D.O. (2010) Synthesis of β-Galactooligosaccharides from Lactose Using Microbial β-Galactosidases. Comprehensive Reviews in Food Science and Food Safety, 9(5): 471-482
Park, A., Oh, D. (2010) Galacto-oligosaccharide production using microbial β-galactosidase: current state and perspectives. Applied Microbiology and Biotechnology, 85(5): 1279-1286
Sangwan, V., Tomar, S.K., Singh, R.R.B., Singh, A.K., Ali, B. (2011) Galactooligosaccharides: Novel Components of Designer Foods. Journal of Food Science, 76(4): R103-R111
Shoaf, K., Mulvey, G. L., Armstrong, G. D., Hutkins, R. W. (2006) Prebiotic Galactooligosaccharides Reduce Adherence of Enteropathogenic Escherichia coli to Tissue Culture Cells. Infection and Immunity, 74(12): 6920-6928
Sinclair, H.R., de Slegte, J., Gibson, G.R., Rastall, R.A. (2009) Galactooligosaccharides (GOS) Inhibit Vibrio cholerae Toxin Binding to Its GM1 Receptor. Journal of Agricultural and Food Chemistry, 57(8): 3113-3119
Torres, D.P.M., Gonçalves, M.do P.F., Teixeira, J.A., Rodrigues, L.R. (2010) Galacto-Oligosaccharides: Production, Properties, Applications, and Significance as Prebiotics. Comprehensive Reviews in Food Science and Food Safety, 9(5): 438-454
Urrutia, P., Rodriguez-Colinas, B., Fernandez-Arrojo, L., Ballesteros, A.O., Wilson, L., Illanes, A., Plou, F.J. (2013) Detailed Analysis of Galactooligosaccharides Synthesis with β-Galactosidase from Aspergillus oryzae. Journal of Agricultural and Food Chemistry, 61(5): 1081-1087
van Leusen, E., Torringa, E., Groenink, P., Kortleve, P., Geene, R., Schoterman, M., Klarenbeek, B. (2014) Industrial Applications of Galactooligosaccharides. u: Moreno, F. Javier; Sanz, María Luz [ur.] Food Oligosaccharides, Chichester, UK: Wiley-Blackwell, str. 470-491
Vera, C., Guerrero, C., Illanes, A. (2011) Determination of the transgalactosylation activity of Aspergillus oryzae β-galactosidase: effect of pH, temperature, and galactose and glucose concentrations. Carbohydrate Research, 346(6): 745-752
Vera, C., Guerrero, C., Conejeros, R., Illanes, A. (2012) Synthesis of galacto-oligosaccharides by β-galactosidase from Aspergillus oryzae using partially dissolved and supersaturated solution of lactose. Enzyme and Microbial Technology, 50(3): 188-194
Warmerdam, A., Boom, R.M., Janssen, A.E. (2013) β-galactosidase stability at high substrate concentrations. SpringerPlus, 2(1):