članak: 1 od 1  
Vojnosanitetski pregled
2004, vol. 61, br. 6, str. 581-588
jezik rada: engleski
originalan članak
doi:10.2298/VSP0406581G
Monoklonsko antitelo R-MC46 stimuliše adheziju i fagocitozu od strane makrofaga pacova
Gašić Sonja, Vučević Dragana, Popović Petar, Vasilijić Saša, Čolić Miodrag J.
Vojnomedicinska akademija

e-adresa: vmaimi@eunet.yu

Sažetak

Uvod. U prethodnim eksperimentima pokazano je da monoklonsko antitelo (mAt) R-MC46, stvoreno u našem Institutu, stimuliše homotipsku agregaciju granulocita pacova i stvaranje proinflamatornih citokina. Cilj ovog rada bio je da se ispitaju ekspresija i funkcija antigena kojega prepoznaje R-MC46 mAt na makrofagama. Metode. Ekspresija antigena R-MC46 je praćena pomoću imunocitohemijskih i floucitometrijskih metoda. Biohemijska karakterizacija antigena je izvršena Western-blot analizom. Sposobnost R-MC46 mAb da moduliše adheziju i fagocitozu ispitivana je pomoću eksperimenata kokultivacije makrofaga sa autolognim timocitima. Rezultati. Pokazano je da se R-MC46 mAt jače vezuje za timusne nego za peritoneumske makrofage. Nakon in vivo tretmana Pristanom kod peritoneumskih makrofaga je uočena značajno veća ekspresija R-MC46 antigena. Western-blot analizom je pokazano da mAt prepoznaje antigen male molekulske mase, od oko 5,5 kDa. R-MC46 mAt je značajno stimulisalo vezivanje i fagocitozu timocita od strane kako timusnih tako i peritoneumskih makrofaga. Ovi procesi bili su kompletno blokirani sa WT.3 (anti-CD18) mAt. Stimulacija vezivanja timocita za makrofage bila je izraženija korišćenjem timusnih makrofaga, dok je fagocitoza ovih ćelija bila veća u prisustvu peritoneumskih makrofaga. Zaključak. R-MC46 mAt prepoznaje novi molekul koji je ispoljen na makrofagama pacova. On je verovatno uključen u procese adhezije i fagocitoze koji su posredovani β2 integrinima, kao i u proinflamatorne funkcije makrofaga.

Ključne reči

fagocitoza; adhezivnost; makrofagi; antitela; monoklonska; antigeni; CD18

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