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2009, vol. 63, br. 1-2, str. 87-101
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Ispitivanje mogućnosti primene preparata na bazi hlor-dioksida za dezinfekciju u pogonima za preradu mleka
Evaluation of possible use of disinfectant based on chlorine dioxide in dairy plant
Univerzitet u Beogradu, Fakultet veterinarske medicine, Katedra za higijenu i tehnologiju namirnica
Sažetak
Loša higijena površina koje dolaze u kontakt sa hranom ima za posledicu kontaminaciju hrane mikroorganizmima, kao što su: Listeria monocytogenes, Salmonella spp., Escherichia coli, Staphylococcus aureus i dr. koji izazivaju alimentarna oboljenja ljudi. Cilj ovog ispitivanja je bio da se utvrdi efikasnost preparata na bazi hlor-dioksida (0,3 % hlor-dioksid) u laboratorijskim uslovima i u zatvorenom sistemu pogona za preradu mleka i proceni mogućnost njegove primene u dezinfekciji površina u pogonima za preradu mleka. Germicidni efekat preparata TwinOxide ispitivan je kvantitativnim suspenzionim testom (BSEN 1276:1997) prema mikroorganizmima: Listeria monocytogenes, Proteus mirabilis, Escherichia coli, Bacillus cereus, Staphylococcus aureus i klinički izolat Pseudomonas aeruginosa. Korozivno dejsto preparata je ispitivano primenom standarda IDF 077:1977. Efikasnost preparata je ispitivana i u zatvorenom sistemu u pogonu za preradu mleka. Preparat na bazi hlor-dioksida pri kontaktnom vremenu od 1 minuta u koncentraciji od 100 ppm, u laboratorijskim uslovima, 100% redukuje >108 cfu/ml L. monocytogenes, E. coli, Proteus mirabilis, Pseudomonas aeruginosa, S. aureus, a u koncentraciji 400 ppm >108 cfu/ml Bacillus cereus. U prisustvu 2 % i 4 % obranog mleka, pri kontaktnom vremenu od 1 minuta, preparat je efikasan u koncentraciji 200 ppm (2%) i 250 ppm (4%), za sve ispitivane mikroorganizme osim za Bacillus cereus, za koji je bila potrebna koncentracija 400 ppm (2 %) i 500 ppm (4 %). Korozivno dejstvo preparata nije utvrđeno. U uslovima primene u zatvorenim sistemima u pogonu za preradu mleka, posle kontakta od 15 minuta, preparat na bazi hlor-dioksida je redukovao broj mikrorganizama, u zavisnosti od mesta primene od 80 do 100%.
Abstract
Poor sanitation of food contact surfaces has been a contributing factor in food borne disease outbreaks, especially those involving Listeria monocytogenes, Salmonella spp., Escherichia coli, Staphylococcus aureus etc. The objectives of this study were therefore to: 1. Determine the efficiency of a disinfectant based on chlorine dioxide in suspension in a closed system in a dairy plant. 2. Evaluate the possibility of disinfection of working surfaces with a disinfectant based on chlorine dioxide. In order to determine the germicidal effect of the disinfectant based on chlorine dioxide by suspension test (BSEN 1276:1997); the following test organisms were used: Listeria monocytogenes, Proteus mirabilis, Escherichia coli, Bacillus cereus, Staphylococcus aureus and Pseudomonas aeruginosa clinical isolate. The corrosive properties of the disinfectant based on chlorine dioxide were tested by IDF 077:1977 standard. The efficacy of this disinfectant was investigated in a closed system in a dairy plant. Results indicated a 100% reduction of >108 cfu/ml L. monocytogenes, E. coli, Proteus mirabilis, Pseudomonas aeruginosa, S. aureus, viable count after 1 minute of exposure to 100 ppm of the disinfectant based on chlorine dioxide and 400 ppm for Bacillus cereus. In the presence of 2% skim milk and 4 % skim milk concentrations of 200 and 250 ppm resulted in 100% reduction in numbers of the five of six test microorganisms, respectively. The spore former, Bacillus cereus is less susceptible to the disinfectant. Therefore, the efficient concentration for 100% reduction in viable count after 1 minute exposure was 500 ppm. The corrosive properties of the disinfectant were not determined. In the case of closed system disinfection in a dairy plant, reduction in viable count after 15 minute exposure to 100 ppm of disinfectant based on chlorine dioxide ranged from 80 to 100%.
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