article: 1 from 1  
Veterinarski glasnik
2010, vol. 64, iss. 1-2, pp. 65-74
article language: Serbian
Original Paper
doi:10.2298/VETGL1002065M
Antibacterial effects of mixtures of extracts of usnea, thyme and angelica obtained using different technological processes against certain types of bacteria of importance in veterinary medicine
Mišić Dušana, Žižović Irenab, Ivanović Jasnab
aUniversity of Belgrade, Faculty of Veterinary Medicine
bUniverzitet u Beogradu, Tehnološko-metalurški fakultet, Katedra za organsku hemijsku tehnologiju


Projekat Ministarstva nauke Republike Srbije, br. TR 19037

Abstract

Antibacterial effects of plant extracts were examined using mixtures of extracts obtained using different technological processes: usnea extract was obtained using the process of supercritical extraction (NKE), angelica extract was obtained through supercritical extraction processes (NKE) and ultrasound extraction using ethanol (UZ), and thyme extract was obtained using the process of hydrodistillation (HD). Mixtures of the listed extracts were examined in various ratios: U (NKE) and T (HD) in a ratio of 1:1, U (NKE) and T (HD) in a ratio of 7:3, U (NKE), T (HD) and A (NKE) in a ratio of 2:2:1, and U (NKE), T (HD) and A (UZ) in a ratio of 2:2:1. The investigations covered 15 strains of bacteria of the genus Staphylococcus, Streptococcus and Enterococus, including the strains MRSA, VRE as well as reference strains of S. pyogenes ATCC 19615, S. agalactiae ATCC 27959 and S. aureus ATCC 11632. The antibacterial action of mixes of plant extracts was examined using the microdilution method in bouillon, and the examined mix concentrations were from 1.25 g/mL to 1280 g/mL. The strongest antibacterial effect was exhibited by mixes of usnea (NKE) and thyme (HD) in ratios of 1:1 and 7:3 with obtained MIC values from 5 g/mL to 160 g/mL, but the MIC value of the listed mixtures for the biggest number of strains amounted to 40 g/mL. A somewhat weaker effect was exhibited by the other examined extract mixtures with obtained MIC values of 10 g/mL to 320 g/mL. Based on the obtained MIC values and the results of previous investigations, it can be concluded that the examined mixtures of plant extracts exhibited a very strong antibacterial effect on the examined bacteria strains.

Keywords

extracts; mixtures; antibacterial effect

References

CrossRefPubMedKoBSON13

Burt, S. (2004) Essential oils: Their antibacterial properties and potential applications in foods: A review. International journal of food microbiology, 94(3): 223-53

CrossRefPubMedKoBSON16

Dorman, H.J., Deans, S.G. (2000) Antimicrobial agents from plants: Antibacterial activity of plant volatile oils. J Appl Microbiol, 88(2): 308-16

CrossRefKoBSON1

Glišić, S., Mišić, D., Stamenić, M., Zizovic, I., Ašanin, R., Skala, D. (2007) Supercritical carbon dioxide extraction of carrot fruit essential oil: Chemical composition and antimicrobial activity. Food Chemistry, 105(1): 346

VBS1

Kišgeci, J. (2008) Lekovite i aromatične biljke. Beograd: Partenon

CrossRefKoBSON

Misic, D., Zizovic, I.T., Stamenic, M.D., Asanin, R.M., Ristic, M.S., Petrovic, S.D., Skala, D.U. (2008) Antimicrobial activity of celery fruit isolates and SFE process modeling. Biochemical Engineering Journal, vol. 42, br. 2, str. 148-152

CrossRefKoBSONScindeks

Mišić, D., Nišavić, J., Žižović, I., Ivanović, J. (2009) Antibakterijsko delovanje i citotoksični efekat natkritičnog ekstrakta timijana i ekstrakta timijana dobijenog procesom hidrodestilacije. Veterinarski glasnik, vol. 63, br. 1-2, str. 17-27

CrossRefKoBSONScindeks

Mišić, D., Ašanin, R., Ivanović, J., Žižović, I. (2009) Ispitivanje antibakterijskog delovanja natkritičnih ekstrakata biljaka kao i ekstrakata dobijenih drugim tehnološkim procesima na neke bakterije izolovane od životinja. Acta veterinaria, vol. 59, br. 5-6, str. 557-568

Najdenova, V., Lisickov, K., Đarmati, Z. (2001) Antimicrobial activity and stability of usnic acid and its derivatives in some cosmetic products, Olaj, Szapan. Kozmetika, 158- 60

35

National Committee for Clinical Laboratory Standards (2008) Performance standards for antimicrobial susceptibility testing. Wayne, Eighteenth informational supplement

CrossRefPubMedKoBSON3

Weckesser, S., Engel, K., Simon-Haarhaus, B., Wittmer, A., Pelz, K., Schempp, C.M. (2007) Screening of plant extracts for antimicrobial activity against bacteria and yeasts with dermatological relevance. Phytomedicine, Vol. 14, No. 7-8, pp. 508-16

PubMedKoBSON

Yamamoto, Y., Miura, Y., Kinoshita, Y., Higuchi, M., Yamada, Y., Murakami, A., Ohigashi, H., Koshimizu, K. (1995) Screening of tissue cultures and thalli of lichens and some of their active constituents for inhibition of tumor promoter-induced Epstein-Barr virus activation. Chemical & pharmaceutical bulletin, 43(8): 1388-90

CrossRefKoBSON1

Žižović, I., Stamenić, M., Orlović, A., Skala, D. (2007) Supercritical carbon dioxide extraction of essential oils from plants with secretory ducts: Mathematical modelling on the micro-scale. Journal of Supercritical Fluids, 39(3): 338