članak: 1 od 1  
Journal of the Serbian Chemical Society
2005, vol. 70, br. 5, str. 753-763
jezik rada: engleski
izvorni naučni članak
doi:10.2298/JSC0505753K
Određivanje kvercetina u farmaceutskim preparatima preko reakcije sa kalijum-titaniloksalatom - određivanje konstanti stabilnosti kvercetin-titaniloksalatnog kompleksa
Kuntić Vesna S.a, Pejić Nataša D.a, Mićić Svetlanaa, Vukojević Vladanab, Vujić Zorica B.a, Malešev Dušan L.a
aUniverzitet u Beogradu, Farmaceutski fakultet
bAlcohol and Drug Addiction Research Section, Department of Clinical Neuroscience, Stockholm, Sweden


Projekat Ministarstva nauke Republike Srbije, br. 1458

Sažetak

Predložen je brz, jednostavan i tačan metod za određivanje kvercetina u farmaceutskim sirovinama i doziranim oblicima.Metod je baziran na spektrofotometrijskom određivanju kompleksa formiranog između kvercetina i kalijum-titaniloksalata u 50 % etanolu. Određene su konstante stabilnosti kvercetin titaniloksalatnog kompleksa potenciometrijski i spektrofotometrijski na različitim temperaturama (T = 26.0oC, 34oC and 39.0oC) i jonskim jačinama (I = 5.0x10-4 mol dm-3, 3.0x10-2 mol dm-3 and 6.0x10-2 mol dm-3) i izračunati su termodinamički parametri. Kako se kvercetin uobičajeno nalazi zajedno sa vitaminom C u doziranim oblicima, predložene su dve procedure za određivanje kvercetina: bez i u prisustvu askorbinske kiseline.U obe predložene procedure, Beer-ov zakon važi u oblasti 00.85 μg mL-1 - 16.9 μg mL-1 kvercetina. Prema prvoj proceduri, molarni apsorpcioni koeficijent kvercetin–titaniloksalatnog kompleksa je a=2.49 x 104 mol-1 dm3 cm-1, Sandelova osetljivost metode je S = 1.35 x 10-2 μg cm-2, a limit detekcije je d = 0.67 μg mL-1. Prema drugoj proceduri, a = 3.04 x 104 mol-1 dm3 cm-1, S = 1.11 x 10-2 μg mL-1. Predloženi metod je primenjen za određivanje kvercetina u doziranim oblicima.

Ključne reči

quercetin; potassium titanyloxalate; spectrophotometric determination; stability constants; dosage form

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