чланак: 1 од 1  
Journal of the Serbian Chemical Society
2008, вол. 73, бр. 12, стр. 1139-1151
језик рада: енглески
изворни научни чланак
doi:10.2298/JSC0812139K

Sinteza mirisnih estara katalizovana lipazama u nevodenoj sredini - optimizacija prinosa pentil-2-metilpropanoata statističkom analizom
aUniverzitet u Beogradu, Tehnološko-metalurški fakultet
bUniverzitet u Beogradu, Mašinski fakultet

е-адреса: zknez@tmf.bg.ac.yu

Projekat Ministarstva nauke Republike Srbije, br. TR-20064

Сажетак

U radu su ispitani uticaji različitih procesnih parametara na sintezu pentil-2-metilpropanoata katalizovanu lipazom iz Candida rugosa. U cilju optimizacije enzimske sinteze estara primenjena je metodologija odzivnih površina u skladu sa odabranim centralnim kompozicionim rotabilnim planom (pet faktora na pet nivoa). Ispitani su uticaji procesnih parametara na prinos estra u sledećim intervalima: početnog sadržaja vode (0,0-2,0 %), temperature (35-75°C), koncentracije enzima (1,0-5,0 g dm-3), početnog molskog udela supstrata (1:2-5:2) i reakcionog vremena (4-48 h). Dobijen je adekvatan matematički model na osnovu koga se može predvideti ponašanje sistema u funkciji ovih pet faktora. Pokazano je da koncentracija enzima, početni molski odnos supstrata i reakciono vreme imaju najveći uticaj na proces, dok sadržaj vode ne utiče značajno na prinos estra. Pod optimalnim uslovima enzimske sinteze ostvaren je prinos estra oko 92%.

Кључне речи

factorial design; surface response analysis; Candida rugosa lipase; flavor esters; esterification

Референце

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