članak: 1 od 1  
Archives of Biological Sciences
2010, vol. 62, br. 1, str. 159-166
jezik rada: engleski
naučni članak
doi:10.2298/ABS1001159M

Antimikrobna aktivnost etarskog ulja Satureja hortensis L. protiv patogenih sojeva mikroorganizama
aUniverzitet u Nišu, Prirodno-matematički fakultet, Odsek Biologija i ekologija
bUniverzitet u Novom Sadu, Prirodno-matematički fakultet, Departman za biologiju i ekologiju
cUniverzitet u Nišu, Medicinski fakultet

Sažetak

Etarsko ulje vrste S. hortensis L., izolovano destilacijom u aparaturi po Clavenger-u, pokazalo je visoku antimikrobnu aktivnost protiv tipskih sojeva (11 bakterijskih i 3 fungalna soja). Testiranje je vršeno poređenjem dveju metoda: disk-difuzione i mikrodilucione. Mikrodilucionom metodom je ustanovljeno da je etarsko ulje vrste S. hortensis L. delovalo protiv svih testiranih gram- negativnih bakterija, u koncentraciji od MIC/MBC=0,025-0,78/0,05-0,78 μl/ml), na koje je referentni antibiotik hloramfenikol delovao u opsegu od MIC/MBC=4,0-16,0 μl/ml. Na ispitivane grampozitivne bakterije ulje je delovalo u opsegu od MIC/MBC=0,05-0,39/0,05- 0,78 μl/ml), što je znatno bolje nego delovanje referentnog antibiotika streptomicina MIC/MBC=0,5-8,0/1,0-16,0 μl/ml. Ulje je ispoljilo visoku antifungalnu aktivnost na sojeve A. niger (MIC/MBC=0,78μl/ml), S. cerevisiae (MIC/MBC=0,39/0,20 μl/ml), kao i na C. albicans (MIC/MBC=0,20 μl/ml). Ovako dobijena visoka antimikrobna aktivnost etarskog ulja S. hortensis L. se može objasniti visokim sadržajem aromatičnog monoterpenskog alkohola karvakrola (67,00%) i ugljovodonika γ-terpinena (15,3%) i p-cimena (6,73%), koji ispoljavaju sinergistički efekat.

Ključne reči

Satureja L.; Satureja hortensis; essential oils; antimicrobial activity

Reference

Adiguzel, A., Ozer, H., Kilic, H., Cetin, B. (2007) Screening of antimicrobial activity of essential oil and methanol extract of Satureja hortensis on foodborne bacteria and fungi. Czech Journal of Food Sciences, 25(2), 81-89
Bagamboula, C.F., Uyttendaele, M., Debevere, J. (2003) Inhibitory effect of thyme and basil essential oils, carvacrol, thymol, estragol, linalool and p-cymene towards Shigella sonnei and S. flexneri. Food Microbiology, Volume Date 2004, 21(1), 33-42
Ball, P.W., Getliffe, F.M. (1972) Acinos Miller. u: Tutin T.G., Heywood V.H., Burges N.A., Moore D.M., Valentine D.H., Walters S.M., Webb D.A. (ur.) Flora Europaea, Cambridge: Cambridge University press, III: 165-166
Baser, K.H.C., Ozek, T., Kirimer, N., Tumen, G. (2004) A comparative study of the essential oils of wild and cultivated satureja hortensis l. Journal of Essential Oil Research, 16, 584-589
Ben, A.A., Combes, S., Preziosi-Belloy, L., Gontard, N., Chalier, P. (2006) Antimicrobial activity of carvacrol related to its chemical structure. Letters in applied microbiology, 43(2): 149-54
Bennis, S., Chami, F., Chami, N., Bouchikhi, T., Remmal, A. (2004) Surface alteration of saccharomyces cerevisiae induced by thymol and eugenol. Letters in Applied Microbiology, 38(6), 454-458
Boyraz, N., Oezcan, M. (2006) Inhibition of phytopathogenic fungi by essential oil, hydrosol, ground material and extract of summer savory (Satureja hortensis L) growing wild in Turkey. International Journal of Food Microbiology, 107(3), 238-242
Chorianopoulos, N., Kalpoutzakis, E., Aligiannis, N., Mitaku, S., Nychas, G., Haroutounian, S.A. (2004) Essential oils of Satureja, Origanum, and Thymus species: chemical composition and antibacterial activities against foodborne pathogens. J Agric Food Chem, 52(26): 8261-7
Deans, S.G., Svoboda, K.P. (1989) Antibacterial activity of summer savory (Satureja hortensis L) essential oil and its constituents. Journal of Horticultural Science, 64, 205-210
di Pasqua, R., Hoskins, N., Betts, G., Mauriello, G. (2006) Changes in membrane fatty acids composition of microbial cells induced by addiction of thymol, carvacrol, limonene, cinnamaldehyde, and eugenol in the growing media. c 54, 2745-2749
Dikbas, N., Kotan, R., Dadasoglu, F., Sahin, F. (2008) Control of Aspergillus flavus with essential oil and methanol extract of Satureja hortensis. International journal of food microbiology, 124(2): 179-82
Dorman, H.J., Deans, S.G. (2000) Antimicrobial agents from plants: Antibacterial activity of plant volatile oils. Journal of applied microbiology, 88(2): 308-16
Dorman, H.J.D., Hiltunen, R. (2003) Fe (III) reductive and free radical-scavenging properties of summer savory (Satureja hortensis L.) extract and subfractions. Food Chemistry, 88 (2), 193-199
Hajhashemi, V., Sadrael, H., Ghannadi, A.R., Mohseni, M. (2000) Antispasmodic and anti-diarrhoeal effect of Satureja hortensis L. essential oil. Journal of Ethnopharmacology, 71(1-2): 187
Hajhashemi, V., Ghannadi, A., Pezeshkian, S.K. (2002) Antinociceptive and anti-inflammatory effects of Satureja hortensis L. extracts and essential oil. Journal of ethnopharmacology, 82(2-3): 83-7
Krist, S., Halwachs, L., Sallaberger, G., Buchbauer, G. (2007) Effects of scents on airborne microbes, part I: Thymol, eugenol, trans-cinnamaldehyde and linalool. Flavour and Fragrance Journal, 22(1): 44
Lambert, R.J., Skandamis, P.N., Coote, P.J., Nychas, G.J. (2001) A study of the minimum inhibitory concentration and mode of action of oregano essential oil, thymol and carvacrol. J Appl Microbiol, 91(3): 453-62
Leporatti, M.L., Ivancheva, S. (2003) Preliminary comparative analysis of medicinal plants used in the traditional medicine of Bulgaria and Italy. Journal of ethnopharmacology, 87(2-3): 123-42
Mihajilov-Krstev, T., Radnovic, D.V., Kitic, D., Zlatkovic, B.K., Ristic, M.S., Brankovic, S. (2009) Chemical composition and antimicrobial activity of Satureja hortensis L. essential oil
National Committee for Clinical Laboratory Standards (2003) Performance standards for antimicrobial susceptibility testing. Wayne, M100-S14.PA:NCCLS
Nostro, A., Blanco, A.R., Cannatelli, M.A., Enea, V., Flamini, G., Morelli, I., Sudano, R.A., Alonzo, V. (2004) Susceptibility of methicillin-resistant staphylococci to oregano essential oil, carvacrol and thymol. FEMS microbiology letters, 230(2): 191-5
Omidbeygi, M., Barzegar, M., Hamidi, Z., Naghdibadi, H. (2007) Antifungal activity of thyme, summer savory and clove essential oils against Aspergillus flavus in liquid medium and tomato paste. Food Control, 18(12): 1518
Oussalah, M., Caillet, S., Saucier, L., Lacroix, M. (2006) Antibacterial effects of selected plant essential oils on the growth of a Pseudomonas putida strain isolated from meat. Meat science, 76, 236-244
Oussalah, M., Caillet, S., Saucier, L., Lacroix, M. (2007) Inhibitory effects of selected plant essential oils on the growth of four pathogenic bacteria: E. coli O157:H7, Salmonella Typhimurium, Staphylococcus aureus and Listeria monocytogenes. Food Control, 18(5): 414
Razzaghi-Abyaneh, M., Shams-Ghahfarokhi, M., Yoshinari, T., Rezaee, M., Jaimand, K., Nagasawa, H., Sakuda, S. (2008) Inhibitory effects of Satureja hortensis L. essential oil on growth and aflatoxin production by Aspergillus parasiticus. International journal of food microbiology, 123(3): 228-33
Ruberto, G., Baratta, M.T. (2000) Antioxidant activity of selected essential oil components in two lipid model systems. Food Chemistry, 69(2): 167
Sahin, F., Karaman, I., Güllüce, M., Oğütçü, H., Sengül, M., Adigüzel, A., Oztürk, S., Kotan, R. (2003) Evaluation of antimicrobial activities of Satureja hortensis L. Journal of ethnopharmacology, 87(1): 61-5
Sefidkon, F., Abbasi, K., Khaniki, G.B. (2006) Influence of drying and extraction methods on yield and chemical composition of the essential oil of Satureja hortensis. Food Chemistry, 99(1): 19
Sefidkon, F., Abbasi, K., Jamzad, Z., Ahmadi, S. (2006) The effect of distillation methods and stage of plant growth on the essential oil content and composition of Satureja rechingeri Jamzad. Food Chemistry, 99(1): 19
Stammati, A., Bonsi, P., Zucco, F., Moezelaar, R., Alkomi, H.I., von Wright, A. (1999) Toxicity of essential oil on microbial and mammalian short-term assays. Food and Chemical Toxicology, 37(8): 813
Šilić, Č. (1979) Monografija rodova Satureja L., Calamintha Miller, Micromeria Bentham, Acinos Miller i Clinopodium L. u flori Jugoslavije. Sarajevo: Zemaljski muzej BiH
Ultee, A., Kets, E.P., Alberda, M., Hoekstra, F.A., Smid, E.J. (2000) Adaptation of the food-borne pathogen Bacillus cereus to carvacrol. Archives of microbiology, 174(4): 233-8
Ultee, A., Bennink, M.H.J., Moezelaar, R. (2002) The phenolichydroxyl group of carvacrol is essential for action against the foodborne pathogen Bacillus cereus. Applied and Environmental Microbiology, 68(4): 1561
Uslu, C., Karasen, R.M., Sahin, F., Taysi, S., Akcay, F. (2003) Effects of aqueous extracts of Satureja hortensis L. on rhinosinusitis treatment in rabbit. Journal of Ethnopharmacology, 88(2-3): 225
Yazdanparast, R., Shahriyary, L. (2008) Comparative effects of Artemisia dracunculus, Satureja hortensis and Origanum majorana on inhibition of blood platelet adhesion, aggregation and secretion. Vascular pharmacology, 48(1): 32-7