članak: 1 od 1  
Archives of Biological Sciences
2010, vol. 62, br. 3, str. 559-564
jezik rada: engleski
naučni članak
doi:10.2298/ABS1003559D

Metagenomska analiza zemljišnih mikrobioloških zajednica
aInstitut za molekularnu genetiku i genetičko inženjerstvo, Beograd
bEmory University, School of Medicine, Department of Biochemistry, Atlanta, Georgia, USA


Projekat Ministarstva nauke Republike Srbije, br. 143056

Sažetak

Ramonda serbica i Ramonda nathaliae, retke biljke 'vaskrsnice' koje rastu na Balkanskom poluostrvu, u odgovoru na stres produkuju velike količine fenola. Bakterijske zajednice poreklom iz rizosfere ovih biljaka analizirane su metagenomskim pristupom. Fluorescentna 'in situ' hibridizacija (FISH) i DAPI bojenje pokazali su da u analiziranim zemljištima ima svega 5% metabolički aktivnih bakterija. Upotrebom prajmera specifičnih za bakterijsku DNK umnoženi su geni za 16S rDNK i konstruisane su dve genske biblioteke. Biblioteke su pretraživane uz pomoć RFLP metode. Od ukupno 192 klona dobijena iz uzorka rizosfere R. nathaliae identifikovano je 35 operativnih taksonomskih jedinica (OTJ), dok je iz uzorka rizosfere R. serbica dobijeno 13 OTJ od ukupno 80 klonova. Predstavnici svake OTJ su sekvencirani. Analizirane zajednice odlikuje veoma mali diverzitet i većina dobijenih sekvenci je pokazala malu sličnost sa DNK sekvencama do sada kultivisanih bakterija.

Ključne reči

metagenome; RFLP; OTU; FISH

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