članak: 1 od 1  
Hemijska industrija
2008, vol. 62, br. 3, str. 217-222
jezik rada: srpski
naučni članak
doi:10.2298/HEMIND0803217K

Efekat ekstrakata akrilata za bazu pločaste zubne proteze na rast HeLa ćelija in vitro
aKlinika za stomatologiju, Odeljenje za stomatološku protetiku, Niš
bMedicinski fakultet, Institut za biomedicinska istraživanja, Niš

e-adresa: ivankostic@sbb.co.yu

Projekat Ministarstva nauke Republike Srbije, br. ON 145072

Sažetak

Ispitivan je rast HeLa ćelija u kulturi in vitro, u različitim koncentracijama ekstrakata 4 akrilatna materijala za izradu baze pločaste zubne proteze. Rast ćelija procenjivan je preko gustine, brojanjem pod invertnim mikroskopom, nakon 48 sati inkubacije i metaboličkim. MTT testom, nakon 3 dana. Ekstrakti su dobijem inkubacijom uzoraka materijala na 37°C u fiziološkom rastvoru, u trajanju od 72 sata. Dobijen je slabiji rast u prisustvu svih materijala. Kod toplotna polimerizujućeg akrilata sa umreživačem primećen je samo pri najvećoj ispitivanoj koncentraciji, od 50% ekstrakta. Hladno polimerizujući akrilat deluje na morfologiju ćelija tako da se u njegovom prisustvu vidi najmanje adherentnog fenotipa.

Ključne reči

akrilati; citotoksicnost; HeLa ćelije

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