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Srpski arhiv za celokupno lekarstvo
2010, vol. 138, br. 9-10, str. 577-583
jezik rada: srpski
vrsta rada: originalan članak
objavljeno: 30/11/2010
doi: 10.2298/SARH1010577S
Uticaj modifikatora metabolizma cikličnih nukleotida na kontraktilnost desne komore srca s očuvanim i uklonjenim endokardnim endotelom
aUniverzitet u Prištini sa privremenim sedištem u Kosovskoj Mitrovici, Medicinski fakultet, Institut za fiziologiju
bUniverzitet u Beogradu, Medicinski fakultet, Institut za medicinsku statistiku i informatiku
cUniverzitet u Prištini sa privremenim sedištem u Kosovskoj Mitrovici, Medicinski fakultet, Institut za histologiju sa embriologijom

e-adresa: srna@eunet.rs

Sažetak

Uvod. Endokardni endotel je prirodna biološka barijera između cirkulišuće krvi u komorama i ćelija srca. Unutrašnja površina srca je potpuno uokvirena endokardnim endotelom, koji pokriva i valvule, a nastavlja se na endotel velikih krvnih sudova. Ima osobinu krvno-srčane barijere, sadrži receptore za brojne medijatore i ima sposobnost sinteze endotelnih medijatora. Cilj rada. Cilj rada je bio da se utvrdi značaj očuvanog endokardnog endotela za kontraktilnost desne komore srca i uticaj modifikatora metabolizma cikličnih nukleotida na kontraktilnost srca s očuvanim i uklonjenim endokardnim endotelom. Metode rada. U eksperimentu su korišćeni odrasli pacovi soja Wistar albino oba pola. Eksperimenti su izvedeni na preparatima desne komore srca pacova kroz dva eksperimentalna modela. Prvi model predstavljao je desnu komoru srca s očuvanim endokardnim endotelom, a drugi s endotelom uklonjenim hemijskim putem potapanjem komore u jednoprocentni rastvor Triton X-100. Rezultati. Kontraktilnost srca s uklonjenim endotelom je bila za 60% manja nego kod srca s očuvanim endokardnim endotelom. Teofilin (10-2 mol/l) ispoljava pozitivan inotropni efekat nezavisno od postojanja endokardnog endotela. Inotropni odgovor može biti indukovan inhibicijom fosfodiesteraze, akumulacijom cikličnih nukleotida i aktivacijom Ca2+ kanala. Modulatorna uloga endokardnog endotela u inotropnim efektima imidazola (2×10-3 mol/l) je značajna (p<0,05). Imidazol utiče na kontraktilnost desne komore samo kada je endokardni endotel očuvan, pa se pretpostavlja da je efekat imidazola posredovan sintezom i oslobađanjem endotelnih medijatora. Zaključak. Očuvani endokardni endotel je neophodan za ostvarivanje kontraktilnih performansi srca.

Ključne reči

endokardni endotel; ciklični adenozinmonofosfat; fosfodiesteraza; teofilin; imidazol

Reference

Abdel-Samad, D., Jacques, D., Perreault, C., Provost, C. (2007) NPY regulates human endocardial endothelial cell function. Peptides, 28(2): 281-7
Andries, L.J., Brutsaert, D.L. (1994) Endocardial endothelium in the rat: junctional organization and permeability. Cell and Tissue Research, 277(3): 391
Bény, J. (1999) Information Networks in the Arterial Wall. News in physiological sciences, 14: 68-73
Boswell-Smith, V., Spina, D., Page, C.P. (2006) Phosphodiesterase inhibitors. British journal of pharmacology, 147 Suppl 1: S252-7
Brunner, F., Andrew, P., Wölkart, G., Zechner, R., Mayer, B. (2001) Myocardial contractile function and heart rate in mice with myocyte-specific overexpression of endothelial nitric oxide synthase. Circulation, 104(25): 3097-102
Brutsaert, D.L., Meulemans, A.L., Sipido, K.R., Sys, S.U. (1988) Effects of damaging the endocardial surface on the mechanical performance of isolated cardiac muscle. Circulation research, 62(2): 358-66
Brutsaert, D.L. (2003) Cardiac endothelial-myocardial signaling: its role in cardiac growth, contractile performance, and rhythmicity. Physiol Rev., 83(1):59-115
Cary, D.A., Mendelsohn, F.A.O. (1987) Effects of forskolin, isoproterenol and IBMX on angiotensin converting enzyme and cyclic AMP production by cultured bovine endothelial cells. Mol Cell Endocrinol., 53(1-2):103-9
Crespo-Otero, R., Bravo-Rodríguez, K., Suardíaz, R., Montero, L.A., García, V.J.M. (2009) Theoretical study of imidazole...NO complexes. journal of physical chemistry. A, 113(52): 14595-605
de Mello, W.C. (1977) Effect of phosphodiesterase inhibitors and dBcAMP on the inotropic and relaxing actions of histamine in cardiac muscle. European journal of pharmacology, 45(2): 153-63
Filippelli, A., Cuparencu, B., Berrino, L., Tomus, C., Rossi, F. (1994) The influence of imidazole administration on arrhythmias induced by potassium chloride, calcium chloride, and ouabain in isolated guinea pig hearts. Current Therapeutic Research, 55(1): 43
Fransen, P., Hendrickx, J., Brutsaert, D.L., Sys, S.U. (2001) Distribution and role of Na(+)/K(+) ATPase in endocardial endothelium. Cardiovascular research, 52(3): 487-99
Gonzalez-Musoz, C., Fuente, T., Hernandez-Cascales, J. (2009) Phosphodiesterases inhibition unmask a positive inotropic effect mediated by β2-adrenoceptors in rat ventricular myocardium. European Journal of Pharmacology, 607(1-3):151-5
Hably, C., Györváry, Z., Bartha, J. (1994) Changes of organ blood flow and cardiac output after imidazole administration. Acta physiologica Hungarica, 82(2): 163-73
Houslay, M.D., Adams, D.R. (2003) PDE4 cAMP phosphodiesterases: Modular enzymes that orchestrate signalling cross-talk, desensitization and compartmentalization. Biochem J., 370(Pt 1), str. 1-18
Kuruvilla, L., Kurtha, C.C. (2003) Molecular mechanisms in endothelial regulation of cardiac function. Mol Cell Biochem, 253(1-2):113-23
Lo, Y., Tsou, H., Lin, R., Wu, D., Wu, B., Lin, Y., Chen, I. (2005) Endothelium-dependent and -independent vasorelaxation by a theophylline derivative MCPT: roles of cyclic nucleotides, potassium channel opening and phosphodiesterase inhibition. Life sciences, 76(8): 931-44
Lugnier, C. (2006) Cyclic nucleotide phosphodiesterase (PDE) superfamily: A new target for the development of specific therapeutic agents. Pharmacology & Therapeutics, 109(3): 366
Osadachii, O., Norton, G., Woodwiss, A. (2005) Inotropic responses to phosphodiesterase inhibitors in cardiac hypertrophy in rats. Eur J Pharmacol, 514(2-3), str. 201-8
Perrone, S.V., Kaplinsky, E.J. (2005) Calcium sensitizer agents: A new class of inotropic agents in the treatment of decompensated heart failure. International journal of cardiology, 103(3): 248-55
Reng, H.P., Dejl, M.M., Riter, D.M., Mur, F.K. (2005) Farmakologija. Beograd: Data status
Ruzsa, K.S. (1974) Relation of the second messenger system to the electrogenesis and regulation of the contraction in the heart cell membrane of insecta. Comp Biochem Physiol A Comp Physiol, 49(1A): 81-8
Varagić, V.M., Milošević, M.P. (2004) Farmakologija. Beograd: Elit Medica
Wu, B., Chen-Chung, I., Lin, R., Chiu, C., An, L., Chen, I. (2005) Aortic smooth muscle relaxants KMUP-3 and KMUP-4, two nitrophenylpiperazine derivatives of xanthine, display cgmp-enhancing activity: Roles of endothelium, phosphodiesterase, and K+ channel. Journal of cardiovascular pharmacology, 46(5): 600-8
Yanaka, N., Kurosawa, Y., Minami, K., Kawai, E., Omori, K. (2003) cGMP-phosphodiesterase activity is up-regulated in response to pressure overload of rat ventricles. Bioscience, biotechnology, and biochemistry, 67(5): 973-9