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Genetika
2008, vol. 40, br. 1, str. 31-38
jezik rada: engleski
izvorni naučni članak
doi:10.2298/GENSR0801031T
Evaluacija Rht gena pšenice korišćenjem molekularnih markera
Tošović-Marić Biljanaa, Kobiljski Borislavb, Obreht Draganaa, Vapa Ljiljanaa
aUniverzitet u Novom Sadu, Prirodno-matematički fakultet
bNaučni institut za ratarstvo i povrtarstvo, Novi Sad

e-adresa: delfinb@neobee.net

Sažetak

Koristeći STSs i SSR markere, u ovom radu izvršena je evaluacija tri, na svetskom nivou najznačajnija major gena reduktora visine, Rht-B1b, Rht-D1b i Rht8. U grupi od 172 genotipa heksaploidne pšenice, poreklom iz više od 20 zemalja, Rht-B1b, Rht-D1b i Rht8 geni nađeni su u 40%, 22% i 62% slučajeva, respektivno. U grupama genotipova domaćeg i stranog porekla najuočljivija razlika u frekvencijama karakterističnih alelnih formi utvrđene su u slučaju Rht8, koji se pokazao kao češći kod domaćih genotipova (78,3%), i Rht-B1b koji je dominirao stranom germplazmom (57,6%). Zastupljenost Rht-D1b bila je gotovo ujednačena u obe grupe sa 22,6% (domaći genotipovi) i 21,2% (strane sorte). Dobijeni rezultati i usvojena metodologija za detekciju ova tri Rht gena od izuzetnog značaja, predstavljaju odličnu polaznu tačku za marker asistiranu selekciju (MAS) u oplemenjivanju visokoprinosnih genotipova pšenice u agroklimatskim uslovima Srbije i područja Mediterana.

Ključne reči

Rht genes; molecular markers; wheat

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