članak: 1 od 1  
Acta veterinaria
2004, vol. 54, br. 5-6, str. 427-435
jezik rada: engleski
neklasifikovan
doi:10.2298/AVB0406427K
Određivanje rezidua sulfonamida u jajima tečnom hromatografijom
Kožárová Ivona, Máté D., Hussein K., Raschmanová Katarína, Marcinčák S., Jevinová Pavlína
University of Veterinary Medicine, Košice

Sažetak

Sulfonamidi se obično primenjuju u tretmanu kokcidioze nosilja u dozi od 2 g/L vode za piće tokom 6 dana. Smatra se da rezidue ovih lekova mogu ostati u jajima i predstavljati potencijalni rizik za zdravlje ljudi. Cilj ovog rada je bio da se utvrde koncentracije sulfonamida u jajima tokom i posle p/o primene sulfonamida, kao i 15 dana od prestanka uzimanja leka. Rezidue sulfonamida su određivane na gradijentu visokih performansi u sistemu za tečnu hromatografiju sa foto-diodnim ARRAY detektorom na 275 nm. Naši rezultati ukazuju da davanje sulfonamida nosiljama dovodi do naglog i stalnog povećanja rezidua sulfonamida u jajima. Najveća koncentracija rezidua sulfonamida je utvrđena 6. dana pri p/o davanju. Nakon prestanka primene leka, koncentracija rezidua naglo pada, ali se one mogu detektovati i 10. dana od prestanka davanja. Jedanaestog dana koncentracije su bile ispod utvrđenog MRL nivoa (0.1 mg.kg-1). Granična kvantifikacija (LOQ) sulfonamida je iznosila 0.3 mg.kg-1 a detektovana granica je bila 0.09 mg.kg-1.

Ključne reči

determination; eggs; HPLC; sulfadimidine residues

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