članak: 1 od 1  
Acta veterinaria
2011, vol. 61, br. 4, str. 339-348
jezik rada: engleski
članak
doi:10.2298/AVB1104339P

Kontraktilnost skeletnog mišića pacova - uloga beta adrenergičkih receptora i sistema azot oksida
aUniverzitet u Beogradu, Medicinski fakultet
bClinic for Physical and Rehabilitation Medicine, Clinical Center of Serbia, Belgrade
cInstitute for Orthopaedic Surgery and Traumatology, Clinical Center of Serbia, Belgrade

e-adresa: mprostran@doctor.com

Projekat Ministarstva nauke Republike Srbije, br. 175023

Sažetak

Značajnu ulogu u modulaciji kontraktilnosti skeletnih mišića igraju i betaadrenoceptori i sistem azot oksida. Adrenoceptori u skeletnom mišiću pretežno pripadaju beta2 podtipu, dok sve tri izoforme sintaze azot oksida mogu uticati na kontraktilnost mišića. Cilj našeg istraživanja je bio da ispitamo eventualne interakcije između beta-agonista sistema azot oksida u modulaciji kontraktilnosti izolovane hemidijafragme pacova. Adrenalin (0,05-1,5 μmol/L) i noradrenalin (1-5 μmol/L), primenjeni kumulativno, dovode do koncentracijski-zavisnog porasta Td. L-NAME (1, 3 i 10 mmol/L; 30 min inkubacije) dovodi do značajnog, doznozavisnog porasta Td mišića koji je pretretiran kumulativnim koncentracijama adrenalina (ΔTd do 16%). Kada je hemidijafragma pretretirana noradrenalinom, a ne adrenalinom, L-NAME (3 mmol/L) je potencirao Td u sličnoj meri. Azot oksid izgleda smanjuje potenciranje kontraktilnosti izazvano aktivacijom betaadrenoceptora, a ishod takve interakcije zavisi od prethodne stimulacije adrenoceptora. Azot oksid verovatno smanjuje efekte stimulacije beta-adrenoceptora putem cGMP-zavisne stimulacije fosfodiesteraze 2. Interakcija između supstanci koje modulišu sistem azot oksida i nivo cAMP-a u skeletnom mišiću može imati značajnu ulogu u lečenju određenih respiratornih i neuroloških oboljenja.

Ključne reči

adrenaline; beta-adrenoceptors; L-NAME; nitricoxide; rat skeletal muscle contractility

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