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2013, vol. 63, br. 1, str. 53-61
Seroepidemiološka detekcija antitela protiv Leptospira spp mikroskopskim aglutinacionim testom kod krava i ovaca u regionu Urmia
Urmia University, Veterinary College, Clinical Science, Iran

e-adresaali_Ramin75@yahoo.com
Ključne reči: cows; grippotyphosa; Leptospirosis; serology; sheep; MAT; pomona
Sažetak
Ova ispitivanja su sprovedena sa ciljem da se utvrdi stepen zastupljenosti, titra antitela i različitost sero-varijeteta Leptospira spp kod 203 krave i 166 ovaca u regionu Urmia na lokalnoj klanici tokom 2011. godine. Uzorci krvi su prikupljani prilikom klanja životinja i izdvajan je krvni serum radi izvođenja seroloških proba mikroskopskim aglutinacionim testom (MAT) antigenima tipičnim za serogrupu L. interrogans: pomona, grypotyphiosa, canicola, hardjo, icterohaemoragia i ballum. Ukupno je 36% krava i 19,3% ovaca ispoljavalo pozitivnu reakciju na najmanje jedan od ispitivanih antigena. Kod krava je bilo najviše reakcija na serovarijetet pomona (22.7%), grypotyphiosa (13,8%) i hardjo (8,4%) a kod ovaca na grypotyphiosa (66,7%), pomona (26.2%) i canicola (7,1%). Reakcije na druge serovarijetete nisu utvrđene. Najčešće registrovane vrednosti titra su iznosile 1:100 i 1:200 kod 18,2% i 26,6% krava i kod 13,5 i 8%ovaca respektivno. Kod 2,3% ovaca utvrđene su vrednosti titra od 1:400. Zastupljenost krava koje reaguju na jedan, dva ili tri serovarijeteta je bio 28,6%, 5,9%% i 1,5%. Ukupno je 13,3% ovaca reagovalo na jedan antigen, a 6% na dva. Razlike vezane za pol životinja nisu utvrđene ali se kod starijih grla zapažao veći broj pozitivnih jedinki (p<0,05). Najčešći mešoviti serovarijeteti su bili: grypotyphiosa/pomona, grypotyphiosa/canicola i canicola/pomona hardjo. Zastupljenost pozitivnih grla ovaca je bila dva puta veća nego kod goveda u regionu Urmia a najzustupljeniji serotipovi kod krava i ovaca su bili pomona i grypotyphiosa respektivno. Većina pozitivnih jedinki je reagovala na jedan sero-varijetet ali je takođe moguća i mešovita infekcija. U regionu Urmia je neophodna stalna kontrola ove opasne zoonoze radi zaštite zdravlja ljudi i životinja.
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O članku

jezik rada: engleski
vrsta rada: neklasifikovan
DOI: 10.2298/AVB1301053R
objavljen u SCIndeksu: 07.11.2013.