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Biotechnology in Animal Husbandry 2005, vol. 21, br. 3-4, str. 13-24
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Identifikacija vrste životinje preko mesa domaćih životinja korišćenjem DNK tehnologije
Nucleic Acid Research Dept., Genetic Engineering & Biotechnology Research Institute (GEBRI), Mubarak City for Scientific Research & Technology Applications, New Borg El-Arab City, Alexandria, Egypt
SažetakTehnika RAPD je korišćena na nekoliko vrsta mesa kako bi se identifikovale vrste životinja od kojih meso police. Ispitivane vrste mesa su od: bivola goveda, koza i ovaca. Osamnaest prajmera kao pojedinačni, kratki oligonukleotidi su korišćeni za otkrivanje vrste i to "fingerprint" tehnikom i genetskim sličnostima kao BS između četiri vrste. Poređenjem sve četiri vrste. vrednosti BS su bile 51.0, 45.0, 59.0, 41.0, 48.0 i 70.0% respektivno. U vezi sa tim, poređenje između koze i ovce je pokazalo visoku genetsku sličnost, dok je između goveda i koze utvrđena niska genetska sličnost. Genetska sličnost kao BS vrednost je varirala od 41.0 do 70.0 % respektivno. Rezultati su pokazali da je RAPD analiza brz i efikasan metod za otkrivanje genetskih varijacija različitih vrsta. Takođe. rezultati su pokazali da je RAPD analiza proizvela jasne "fingerprint" podatke za proizvode koji su analizirani i kod kojih je vrsta mogla lako da se identifikuje. Dobijeni rezultati su potvrdili sposobnost RAPD analize u identifikovanju vrste mesa uzoraka različitog porekla i koji su bili podvrgnuti različitim proizvodnim uslovima. lako je pitanje tehnike koja će se koristiti stvar izbora ako DNK nije ozbiljno razgrađena na fragmente od ispod nekoliko stotina baznih prajmera. Za vrste korišćene u ovom radu RAPD je bila pouzdana, lakša, brža i jeftinija tehnika u poređenju sa ostalim tehnikama koje se baziraju na DNK.
Ključne reči
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