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Biotechnology in Animal Husbandry
2006, vol. 22, br. 1-2, str. 35-46
jezik rada: engleski
izvorni naučni članak
doi:10.2298/BAH0602035R
Mapiranje lokusa kvantitativnih osobina koji utiču na dnevni prirast telesne mase na hromozomu 6 kod nemačke holštajn populacije
Reißmann M., Reinecke P., Müller U., Abdel-Rahman S.
Department of breeding biology and molecular animal breeding, Institute of livestock sciences, Faculty of agriculture and horticulture, Humboldt University, Berlin, Germany

Sažetak

Dvanaest mikrosatelitskih markera odabranih iz različitih mapa veza na hromozomu 6 su analizirani u 15 monozitgotnih (MZ) blizanačkih parova nemačke holštajn populacije kako bi se otkrili i locirali QTL/ lokusi kvalitativnih osobina koji utiču na osobine dnevnog prirasta telesne mase (DBWG), korišćenjem opšteg linearnog modela u SPSS programu. Signifikantni efekti dvanaest markera mikrosatelita na hromozomu 6 su genotipizirani i ocenjeni u odnosu na dnevni prirast telesne mase (DBWG). Tri markera BMS2508, BM3026 i TGLA37 na tri različite pozicije na hromozomu 6 su bili povezani sa signifikantnim uticajima na dnevni prirast telesne mase. Poređenje ovog rezultata i prethodno identifikovanog QTL govori u prilog tvrdnji da je lokacija QTL-a za osobine porasta na hromozomu 6, dok je signifikantni QTL za masu pri rođenju prethodno identifikovana na hromozomu 6 u bliskoj vezi sa markerom BM3026. Rezultati dobijeni ovim istraživanjem ukazuju na postojanje putativnog QTL-a za DBWG u populaciji nemačkog holštajna na hromozomu 6 u razmaku od 15.2 cM koji je okružen markerima BMS2508 i TGLA37. Rezultati ovog istraživanja se mogu koristiti u radu na finom mapiranju koji će uslediti i mogu se primeniti u selekciji proizvodnih osobina koja se bazira na markerima - marker-assisted selection (MAS).

Ključne reči

cattle; body weight; microsatellite markers; association analysis

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