članak: 1 od 1  
Biotechnology in Animal Husbandry
2010, vol. 26, br. 3-4, str. 215-223
jezik rada: engleski
pregledni članak
doi:10.2298/BAH1004215A
Molekularno-fenotipska analiza efikasnosti ukrštanja na proizvodnju mesa lokalnih linija pilića
Aly O.M.a, Ahmed M.M.b, Abdel-Rahman S.M.c
aEl-Sabhiah Poultry Research Station, Alexandria, Animal Production Research Institute, Dokki, Cairo, Egypt
bGenetic Engineering & Biotechnology Research Institute, Mubarak City for Scientific Research and Technology Applications, Alexandria, Egypt + Department of Biological Sciences, Faculty of Science, King Abdul-Aziz University, Kingdom of Saudi Arabia
cGenetic Engineering & Biotechnology Research Institute, Mubarak City for Scientific Research and Technology Applications, Alexandria, Egypt

e-adresa: salahmaa@yahoo.com

Sažetak

DNK je ekstrahovana iz uzoraka krvi lokalnih linija pilića: Bandara, Gimmizah, i njihovih meleza (Bandara x Gimmizah i Gimmizah x Bandara). RAPD-PCR tehnika je korišćena za otkrivanje genetskih sličnosti, deljenih traka (band sharing - BS) među hibridima-melezima i njihovim roditeljima korišćenjem prajmera devet kratkih oligonukleiotida. Genetska sličnost izražena kao BS vrednosti je izračunata u granicama od 68 do 91%. Bandara muški pilići sa kokicama Gimmizah su pokazali pozitivni heterozis u uzrastu of 8 nedelja. Međutim, dokazi dobijeni u ovom istraživanju ukazuju da se ukrštanjem lokalnih linija može veoma brzo poboljšati telesna masa potomstva tokom komercijalnog uzrasta. Ovo istraživanje održava sposobnost RAPD-PCR za utvrđivanje povezanosti između genetske sličnosti ispoljenu kroz BS vrednosti i heterozis (hibridni vigor).

Ključne reči

chicken; crosses; heterosis; RAPD-PCR; genetic similarity

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