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2015, vol. 31, br. 4, str. 467-480
Reproduktivna biotehnologija u stočarstvu - trenutni status i budući izgledi
Institute of Biology and Immunology of Reproduction 'acad. Kiril Bratanov' - Bulgarian Academy of Sciences, Sofia, Bulgaria

e-adresakichevamar@abv.bg
Projekat:
Grant BG051PO001/3.3-05-0001 'Science and Business', Bulgarian Ministry of Education and Science

Ključne reči: buffalo; boar; dog; seminal plasma proteins
Sažetak
Do danas još uvek ne postoji optimalna biotehnologija koja osigurava maksimalno očuvanje funkcionalnih parametara spermatozoida bivola, svinja i pasa. Cilj ovog istraživanja je da se ispita biološki potencijal proteina semene plazme, koji su specifični samo za ejakulate sa visokim kriotolerancijom, i parametara kvaliteta spermatozoida. Parametri pokretljivosti i brzine spermatozoida su ocenjeni uz pomoć kompjuterske analize sperme. Proteini plazme sperme su razdvojeni ekskluzijom po veličini, tečnom hromatografijom i karakterisani poliakrilamidnom gel elektroforezom i masenom spektrometrijom. Na osnovu dobijenih rezultata, stvoreni su diluenti sperme i metode za biološku evaluaciju potencijala oplodnje spermatozoida bivola, svinje i pasa i predloženi za praktičnu primenu.
Reference
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Daskalova, D., Kirilova, I., Hristova, M., Gradinarska, D., Lazov, K., Ivanova-Kicheva, M. (2015) Identification of seminal plasma proteins related to low cryotolerance of boar spermatozoa. American Journal of Reproductive Immunology, 73, Suppl; 23-49; 1
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O članku

jezik rada: engleski
vrsta rada: izvorni naučni članak
DOI: 10.2298/bah1504467I
objavljen u SCIndeksu: 03.03.2016.

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