Kragujevac Journal of Science
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2019, br. 41, str. 77-86
Altered apoptosis and biotransformation signaling in HCT-116 colorectal carcinoma cells induced by Teucrium chamaedrys L. extract
(naslov ne postoji na srpskom)
Univerzitet u Kragujevcu, Prirodno-matematički fakultet, Institut za biologiju i ekologiju, Smer Biologija - ekologija, Srbija

Preklinička ispitivanja bioaktivnih supstanci (MPNTR - 41010)

Ključne reči: anticancer properties; apoptosis; biotransformation; food supplement, plant extract; Teucrium
(ne postoji na srpskom)
The aim of this study was an investigation of pro-apoptotic activity of methanol extract from T. chamaedrys, a more detailed determination of the signal molecules activated in the process of apoptosis, and effects on mRNA expression of enzymes involved in biotransformation (CYP1A1 and GSTP1) and membrane transporter, MRP-2 in HCT-116 colorectal carcinoma cells. The results show pronounced proapoptotic activity of T. chamaedrys extract, due to activation of both extrinsic and intrinsic pathways. The death receptor associated signaling pathway was activated in HCT-116 following treatment by T. chamaedrys, via increased Fas receptor expression and activity of caspase 8. Activation of caspase 9 suggests that mitochondrial signalling also has an impact. The extract reduced mRNA expression of GSTP1 and MRP-2 genes, as one of the causes of multi drug resistance in cancer cells. Observed results offer the possibility for the use of T. chamaedrys extract in the context of cancer prevention and therapy.
Abu-Dahab, R., Afifi, F. (2007) Antiproliferative activity of selected medicinal plants of Jordan against a breast adenocarcinoma cell line (MCF7). Scientia Pharmaceutica, 75(3): 121-136
Baskic, D., Popovic, S., Ristic, P., Arsenijevic, N. (2006) Analysis of cycloheximide-induced apoptosis in human leukocytes: Fluorescence microscopy using annexin V/propidium iodide versus acridin orange/ethidium bromide. Cell Biology International, 30(11): 924-932
Bishayee, A. (2012) Editorial: Current advances in cancer prevention and treatment by natural products. Current Pharmacy and Biotechnology, 13: 115-116
Bulus, H., Oguztuzun, S., Güler, S.G., Kilic, M., Ada, O.A., Göl, S., Kocdogan, K.A., Kaygın, P., Bozer, B., Iscan, M. (2019) Expression of CYP and GST in human normal and colon tumor tissues. Biotechnic & Histochemistry, 94(1): 1-9
Bustin, S.А. (2000) Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays. Journal of Molecular Endocrinology, 25: 169-193
Choi, E., Lee, S., Nhung, B.C., Suh, M., Park, B., Jun, J.K., Choi, K.S. (2017) Cancer mortality-to-incidence ratio as an indicator of cancer management outcomes in Organization for Economic Cooperation and Development countries. Epidemiology and Health, 39: e2017006-e2017006
Chomczynski, P., Sacchi, N. (1987) Single-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate-Phenol-Chloroform Extraction. Analytical Biochemistry, 162(1): 156-159
Cragg, G.M., Newman, D.J. (2013) Natural products: A continuing source of novel drug leads. Biochimica et Biophysica Acta (BBA) - General Subjects, 1830(6): 3670-3695
Ćurčić, M.G., Stanković, M.S., Cvetković, D., Topuzović, M., Marković, S.D. (2014) Ligustrum vulgare L.: In vitro free radical scavenging activity and pro-oxidant properties in colon cancer cell lines. Digest Journal of Nanomaterial Bioscience, 9(4): 1689-1697
Ćurčić, M.G., Stanković, M.S., Mrkalić, E.M., Matović, Z.D., Banković, D.D., Cvetković, D.M., Đačić, D.S., Marković, S.D. (2012) Antiproliferative and Proapoptotic Activities of Methanolic Extracts from Ligustrum vulgare L. as an Individual Treatment and in Combination with Palladium Complex. International Journal of Molecular Sciences, 13(2): 2521-2534
Elmastas, M., Erenler, R., Isnac, B., Aksit, H., Sen, O., Genc, N., Demirtas, I. (2016) Isolation and identification of a new NEO-clerodane diterpenoid fromTeucrium chamaedrys L. Natural Product Research, 30(3): 299-304
Fridlender, M., Kapulnik, Y., Koltai, H. (2015) Plant derived substances with anti-cancer activity: From folklore to practice. Frontiers in Plant Science, 6: 799-799
Fulda, S., Galluzzi, L., Kroemer, G. (2010) Targeting mitochondria for cancer therapy. Nature Reviews Drug Discovery, 9(6): 447-464
Gali-Muhtasib, H., Hmadi, R., Kareh, M., Tohme, R., Darwiche, N. (2015) Cell death mechanisms of plant-derived anticancer drugs: Beyond apoptosis. Apoptosis, 20(12): 1531-1562
Guesmi, F., Tyagi, A.K., Prasad, S., Landoulsi, A. (2018) Terpenes from essential oils and hydrolate of Teucrium alopecurus triggered apoptotic events dependent on caspases activation and PARP cleavage in human colon cancer cells through decreased protein expressions. Oncotarget, 9(64): 25955-25955
Hassan, M., Watari, H., Abualmaaty, A., Ohba, Y., Sakuragi, N. (2014) Apoptosis and Molecular Targeting Therapy in Cancer. BioMed Research International, 2014: 1-23
Housman, G., Byler, S., Heerboth, S., Lapinska, K., Longacre, M., Snyder, N., Sarkar, S. (2014) Drug Resistance in Cancer: An Overview. Cancers (Basel), 6(3): 1769-1792
Jain, A.K., Jain, S., Rana, A.C. (2007) Metabolic enzyme considerations in cancer therapy. Malaysian Journal of Medical Sciences, 14(1): 10-17
Lichota, A., Gwozdzinski, K. (2018) Anticancer Activity of Natural Compounds from Plant and Marine Environment. International Journal of Molecular Sciences, 19(11): 3533-3533
Lin, L.Z., Harnly, J.M., Upton, R. (2009) Comparison of the phenolic component profiles of skullcap (Scutellaria lateriflora) and germander (Teucrium canadenseandT. chamaedrys): A potentially hepatotoxic adulterant. Phytochemical Analysis, 20(4): 298-306
Manure, J.Y., Naikwade, N.S. (2018) A Review on: Plants as Anticancer. Pharma Tutor, 6(2): 40-40
Mathur, S., Hoskins, C. (2017) Drug development: Lessons from nature. Biomedical Reports, 6(6): 612-614
Milutinović, M., Stanković, M., Cvetković, D., Maksimović, V., Šmit, B., Pavlović, R., Marković, S. (2015) The Molecular Mechanisms of Apoptosis Induced byAllium flavum L. and Synergistic Effects with New-Synthesized Pd(II) Complex on Colon Cancer Cells. Journal of Food Biochemistry, 39(3): 238-250
Mukanganyama, S., Bezabih, M., Robert, M., Ngadjui, B.T., Kapche, G.F.W., Ngandeu, F., Abegaz, B. (2011) The evaluation of novel natural products as inhibitors of human glutathione transferase P1-1. Journal of Enzyme Inhibition and Medicinal Chemistry, 26(4): 460-467
Nikodijević, D., Milutinović, M., Cvetković, D., Stanković, M., Živanović, M.N., Marković, S. (2016) Effects of Teucrium polium L. and Teucrium montanum L.: Extracts on mechanisms of apoptosis in breast and colon cancer cells. Kragujevac Journal of Science, br. 38, str. 147-159
Nurgali, K., Jagoe, T.R., Abalo, R. (2018) Editorial: Adverse Effects of Cancer Chemotherapy: Anything New to Improve Tolerance and Reduce Sequelae?. Frontiers in Pharmacology, 9: 245-245
Parrish, A.B., Freel, C.D., Kornbluth, S. (2013) Cellular Mechanisms Controlling Caspase Activation and Function. Cold Spring Harbor Perspectives in Biology, 5(6): a008672-a008672
Pathania, S., Bhatia, R., Baldi, A., Singh, R.K., Rawal, R.K. (2018) Drug metabolizing enzymes and their inhibitors' role in cancer resistance. Biomedicine & Pharmacotherapy, 105: 53-65
Pfeffer, C.M., Singh, A.T.K. (2018) Apoptosis: A Target for Anticancer Therapy. International Journal of Molecular Sciences, 19(2): 448-448
Sodani, K., Patel, A., Kathawala, R.J., Chen, Z.S. (2012) Multidrug resistance associated proteins in multidrug resistance. Chinese Journal of Cancer, 31(2): 58-72
Stankovic, M.S., Curcic, M.G., Zizic, J.B., Topuzovic, M.D., Solujic, S.R., Markovic, S.D. (2011) Teucrium Plant Species as Natural Sources of Novel Anticancer Compounds: Antiproliferative, Proapoptotic and Antioxidant Properties. International Journal of Molecular Sciences, 12(7): 4190-4205
Stanković, M., Topuzović, M., Marković, A., Pavlović, D., Solujić, S., Nićiforović, N., Mihailović, V. (2010) Antioxidant activity, phenol and flavonoid contents of different Teucrium chamaedrys L. exstracts. Biotechnology & Biotechnological Equipment, Second Balkan Conference, Plovdiv, Bulgaria 24, 21-23
Stanković, M.S., Mitrović, T.Lj., Matić, I.Z., Topuzović, M.D., Stamenković, S.M. (2015) New Values of Teucrium species: In Vitro Study of Cytotoxic Activities of Secondary Metabolites. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 43(1): 41-46
Suffnes, M., Pezzuto, J.M. (1990) Assays related to cancer drug discovery. u: Hostettman, K. [ur.] Methods in Plant Biochemistry: Assay for Bioactivity, London: Academic Press, 71-133
Tarhan, L., Nakipoğlu, M., Kavakcıoğlu, B., Tongul, B., Nalbantsoy, A. (2016) The Induction of Growth Inhibition and Apoptosis in HeLa and MCF-7 Cells by Teucrium sandrasicum, Having Effective Antioxidant Properties. Applied Biochemistry and Biotechnology, 178(5): 1028-1041
Thomford, N.E., Senthebane, D.A., Rowe, A., Munro, D., Seele, P., Maroyi, A., Dzobo, K. (2018) Natural Products for Drug Discovery in the 21st Century: Innovations for Novel Drug Discovery. International Journal of Molecular Sciences, 19(6): 1578-1578
Wong, S.Y.R. (2011) Apoptosis in cancer: From pathogenesis to treatment. Journal of Experimental & Clinical Cancer Research, 30(1): 87-87
Zhai, W., Jeong, H., Cui, L., Krainc, D., Tjian, R. (2005) In Vitro Analysis of Huntingtin-Mediated Transcriptional Repression Reveals Multiple Transcription Factor Targets. Cell, 123(7): 1241-1253
Zhao, G., Liu, C., Wang, R., Song, D., Wang, X., Lou, H., Jing, Y. (2007) The synthesis of α,β-unsaturated carbonyl derivatives with the ability to inhibit both glutathione S-transferase P1-1 activity and the proliferation of leukemia cells. Bioorganic & Medicinal Chemistry, 15(7): 2701-2707

O članku

jezik rada: engleski
vrsta rada: izvorni naučni članak
DOI: 10.5937/KgJSci1941077M
objavljen u SCIndeksu: 04.07.2019.
Creative Commons License 4.0

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