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2015, vol. 52, br. 2, str. 85-89
Mogućnost primene SSR i ISSR markera u proceni DNK polimorfizma unutar roda Vicia
Naučni institut za ratarstvo i povrtarstvo, Novi Sad

e-adresaksenija.ajdukovic@nsseme.com
Projekat:
Povećanje tržišnog značaja krmnih biljaka oplemenjivanjem i optimizacijom tehnologije proizvodnje semena (MPNTR - 31024)

Ključne reči: DNK polimorfizam; ISSR; molekularni markeri; SSR; Vicia
Sažetak
Razvoj markera obično zahteva vreme i velika materijalna ulaganja. Umesto razvijanja novih markera za manje proučavane vrste, alternativa može biti transfer sa vrsta unutar istog roda. Cilj ovog rada je bio da se proceni mogućnost primene SSR prethodno razvijenih na Vicia faba i ISSR prajmera u proceni DNK polimorfizma u rodu Vicia. Prenosivost SSR markera se kretala u intervalu 25-100% sa prosečnom vrednošću od 56,25%. Iako su produkti tri SSR prajmera bili polimorfni, zbog dobijene količine i kvaliteta, njihovo korišćenje u daljim istraživanjima je diskutabilno. Svi ispitivani ISSR prajmeri su davali različite profile amplifikacije za svaku ispitivanu vrstu. Prosečni procenat polimorfizma ISSR prajmera je bio vrlo visok (93,75%), što sugeriše da ovi prajmeri mogu biti korišćeni u proceni DNK polimorfizma unutar roda Vicia.
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O članku

jezik rada: engleski
vrsta rada: izvorni naučni članak
DOI: 10.5937/ratpov52-8008
objavljen u SCIndeksu: 24.06.2015.
metod recenzije: dvostruko anoniman

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