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Glasnik Šumarskog fakulteta
2015, br. 111, str. 99-112
jezik rada: srpski, engleski
vrsta rada: izvorni naučni članak
doi:10.2298/GSF1511099M


Mikropropagacija Achillea millefolium L. - na redukovanoj MS podlozi i mogućnost aklimatizacije izdanaka dobijenih in vitro istovremeno sa ožiljavanjem u hidroponskoj kulturi
Univerzitet u Beogradu, Šumarski fakultet

e-adresa: marija.markovic@sfb.bg.ac.rs

Projekat

Istraživanje klimatskih promena i njihovog uticaja na životnu sredinu - praćenje uticaja, adaptacija i ublažavanje (MPNTR - 43007)

Sažetak

Kako bi se uspostavio efikasan sistem proizvodnje lekovite vrste A. millefolium, cilj ovog istraživanja je bio ispitivanje mogućnosti njene mikropropagacije na redukovanoj MS podlozi i zatim direktne aklimatizacije dobijenih izdanaka u hidroponskoj kulturi. Korišćena su dva tipa eksplanata - vršne i bazalne reznice, a povoljniji rezultati su postignuti sa vršnim reznicama. Razvoj izdanaka u fazi multiplikacije je bio uspešan, a procenat regeneracije visok (100%). Ožiljavanje ex vitro istovremeno sa aklimatizacijom u modifikovanom Hoglandovom rastvoru je bilo uspešno (83%), ali je procenat ožiljavanja vršnih reznica in vitro na redukovanoj MS podlozi bio veći (95%). S obzirom da se izostavljanjem faze ožiljavanja in vitro postupak proizvodnje A. millefolium znatno skraćuje, potrebno je dodatnim istraživanjima optimizirati sastav hranljivog rastvora u hidroponskoj kulturi, njegovu EC i pH, za potrebe plantažnog uzgoja selekcionisanih genotipova.

Ključne reči

hajdučka trava; mikropropagacija; ožiljavanje ex vitro; hidroponski uzgoj

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