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Scripta Medica
2019, vol. 50, br. 2, str. 97-101
jezik rada: engleski
vrsta rada: izvorni naučni članak
objavljeno: 13/08/2019
doi: 10.5937/scriptamed50-22635
Molecular typing of RhD-negative blood donors with C and/or E antigen
(naslov ne postoji na srpskom)
aInstitute for Transfusion Medicine of the Republic of Srpska, Banja Luka, Bosnia and Herzegovina + Univerzitet u Banjoj Luci, Medicinski fakultet, Republika Srpska, BiH
bInstitute for Transfusion Medicine of the Republic of Srpska, Banja Luka, Bosnia and Herzegovina
cJosip Juraj Strossmayer University of Osijek, Osijek, Croatia



(ne postoji na srpskom)
Background: Most people are either RhD positive or RhD negative, but there is also a number of persons with D antigen variants. The aim of this study was to prove, by using molecular diagnostic tests, whether the RHD gene and D antigen on the red cell membrane of the blood donors serologically typed as RhD-negative with RhD phenotype Ccddee and ccddEe, are so weak that they cannot be proven by serology techniques or the available anti-D test serums. Methods: Samples used are those of regular voluntary donors who were serotyped as RhD-negative, C and/or E positive. Samples were collected from voluntary donors at the Institute for Transfusion Medicine of the Republic of Srpska during the period from April 2016 to December 2018. Results: Among the serologically proven RhD-negative donors, 346 had C and/or E in their phenotype and those were subjected to molecular screening test. Conclusion: The first results of molecular typing match those published in literature, i.e. the RHD gene is present in some serologically RhD-negative forms, which was proven by molecular testing.

Ključne reči


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