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2020, vol. 26, iss. 1, pp. 38-51
Biocontrol of economically significant diseases in order to increase the yield of pot marigold and valerian seeds and potato tubers
aInstitute for Medicinal Plant Research 'Dr. Josif Pančić ', Belgrade
bInstitute of Soil Sciences, Belgrade
cBayer CropScience, Beograd
dPSS Institut "Tamiš", Pančevo
eInstitute of Field and Vegetable Crops, Novi Sad
fUniversity of Belgrade, Faculty of Technology and Metallurgy
Ministry of Education, Science and Technological Development, Republic of Serbia (Institution: Institute for Medicinal Plant Research 'Dr. Josif Pančić ', Belgrade) (MESTD - 451-03-68/2020-14/200003)

Keywords: pot marigold; valerian; potato; economically significant diseases; preparations
The paper examines the effect of the application of three different preparations on the presence of four economically significant diseases in the crops of pot marigold, valerian and potato. On pot marigold, the intensity was determined of pot marigold powdery mildew infection of the marigold leaf (Podosphaera xanthii (Castagne) U. Braun & Shishkoff), whereas on valerian, it was the intensity of valerian stem canker and black mold (Alternaria alternata (Fr. ex Fr.) Keissel), and in potatoes, the intensity of early blight (Alternaria solani (Ellis & Mart.)) and that of late blight (Phytophthora infestans (Mont.) de Bary). Of the preparations, the synthetic preparation "Sekvenca" ("Sequence") (a.m. difenoconazole, 250 g/l) was applied at a dose of 0.5 l/ha, the registered biostimulant "Zlatno inje" ("Golden frost") (manure-based fertiliser) was applied at a dose of 3.0 l/ha, microbiological preparation (Bacillus amyloliquefaciens ssp. TMF3) at a dose of 3.0 l/ha. The fourth variant was the control variant, i.e. the variant without treatment. Field experiments were performed during 2019 at two locations in the wider area of the City of Pančevo. The experiments were performed by a randomised block system with three replications. The size of the main plot was 9.0 m2 (4.5 x 2.0 m). The following was used as plant material: the pot marigold variety of "Domaći oranž" ("Domestic orange"), the valerian variety of "Vojvođanski" ("Vojvodinian") and the potato variety of "Desire". The first assessment of disease intensity was performed one month following the treatment with the selected preparations, and the second one was performed two weeks after the first assessment. In the pot marigold crops, the smallest infection percentage had the plants treated with the type Bacillus amyloliquefaciens TMF3, in valerian, the fewest plants infected with the fungus A. alternata were recorded on small plots on which the synthetic preparation "Sekvenca" was applied, which was also the case with early blight and late blight. The weakest efficiency in the control of pot marigold powdery mildew infection and valerian stem canker and black mold was recorded with the use of the biostimulant "Zlatno inje", which showed the highest efficiency in the case of late blight. The highest yield of pot marigold seeds (632.7 kg/ha) had been treated with the type Bacillus amyloliquefaciens TMF3, with valerian, the highest seeds yield was recorded following the application of the preparation "Sekvenca" (186.1 kg/ha), while in potatoes, the highest amount of tubers was recorded with the preparation "Zlatno inje" (30.83 t/ha). However, if the agroecological and the agrotechnical aspects of production are taken into account, by the use of the type Bacillus amyloliquefaciens TMF3, a satisfactory basis was achieved for various kinds of further research of this and similar preparations in order to improve the existing production of medicinal and other types of plants.
Abd-El-khair, H., Haggag, W.M. (2007) Application of some Egyptian medicinal plant extracts against potato late and early blights. Res. J. Agric. Biol. Sci, 3(3): 166-175
Ali, G.S., El-Sayed, A.S.A., Patel, J.S., Green, K.B., Ali, M.S., Brennan, M., Norman, D. (2016) Ex Vivo Application of Secreted Metabolites Produced by Soil-Inhabiting Bacillus spp. Efficiently Controls Foliar Diseases Caused by Alternaria spp. Applied and Environmental Microbiology, 82(2): 478-490
Andersen, B., Nielsen, K.F., Fernández, P.V., Patriarca, A. (2015) Characterization of Alternaria strains from Argentinean blueberry, tomato, walnut and wheat. International Journal of Food Microbiology, 196: 1-10
Aslam, A., Naz, F., Arshad, M., Qureshi, R., Rauf, C.A. (2010) In vitro antifungal activity of selected medicinal plant diffusates against Alternaria solani, Rhizoctonia solani and Macrophomina phaseolina. Pak. J. Bot, 42(4), str. 2911-2919
Bernath, J. (1997) Cultivation of valerian. in: Houghton P. [ed.] Valerian: The Genus Valeriana (Medicinal and Aromatic Plants: Industrial Profiles), CRC Press, Edition: 1. ISBN: 9057021706, 77-90
Chen, J., Zou, X., Liu, Q., Wang, F., Feng, W., Wan, N. (2014) Combination effect of chitosan and methyl jasmonate on controlling Alternaria alternata and enhancing activity of cherry tomato fruit defense mechanisms. Crop Protection, 56: 31-36
Chowdappa, P., Mohan, K.S.P., Lakshmi, J.M., Upreti, K.K. (2013) Growth stimulation and induction of systemic resistance in tomato against early and late blight by Bacillus subtilis OTPB1 or Trichoderma harzianum OTPB3. Biological Control, 65(1): 109-117
Dimitrijevic, S.M., Radanovic, D.S., Antic-Mladenovic, S.B., Milutinovic, M.D., Rajilic-Stojanovic, M.D., Dimitrijevic-Brankovic, S.I. (2017) Enhanced fertilization effect of a compost obtained from mixed herbs waste inoculated with novel strains of mesophilic bacteria. Hemijska industrija, vol. 71, br. 6, str. 503-513
Dragičević, V., Nikolić, B., Waisi, H., Stojiljković, M., Đurović, S., Spasojević, I., Perić, V. (2015) Alterations in mineral nutrients in soybean grain induced by organomineral foliar fertilizers. Chemical and Biological Technologies in Agriculture, 2(1): 1-8
Đisalov, J. (2015) Identifikacija Alternaria spp. na zrnu spelte i uticaj zaraze na komponenete prinosa, sadržaj mikotoksina i tehnološki kvalitet. Novi Sad: Univerzitet u Novom Sadu - Tehnološki fakultet, Doktorska disertacija
Đorđević, M., Đorđević, O., Đorđević, R., Mijatović, M., Kostić, M., Todorović, G., Ivanović, M. (2013) Alternative approach in control in tomato pathogen by using essential oils in vitro. Pak. J. Bot., 45(3): 1069-1072
Đorđević, M., Šević, M., Mijatović, M., Todorović, G., Kostić, M. (2011) In vitro effectiveness of different essential oils in control of Alternaria alternata. Zaštita bilja / Plant Protection, vol. 62, br. 3, str. 159-168
El-Morsy, S., Shalaby, I.A. (2013) Control of chamomile powdery mildew disease using potassien, citrien and chitosan. Journal of Plant Protection and Pathology, 4(11): 985-997
Fakirova, V.I. (1991) Fungi Bulgaricae: Ordo Erysiphales. in: Aedibus Academiae Scientiarum Bulgariae, Sofia, Vol. 1
Fernández, C.I., Leblon, B., Haddadi, A., Wang, K., Wang, J. (2020) Potato Late Blight Detection at the Leaf and Canopy Levels Based in the Red and Red-Edge Spectral Regions. Remote Sensing, 12(8): 1292-1292
Filipović, V., Ugrenović, V. (2015) Pot Marigold: One of the More Demanded Medicinal Plants. in: Subić Jonel, Kuzman Boris, Jean Vasile Andrei [ed.] International Scientific Meeting: Sustainable agriculture and rural development in terms of the Republic of Serbia strategic goals realization within the Danube region: Regional specifics, December 10, Belgrade: Institute of Agricultural Economics, 296 - 313
Filipović, V., Ugrenović, V., Radanović, D., Marković, T., Popović, V., Aćimović, M., Sikora, V. (2016) Morphological features, productivity and quality of pot marigold (Calendula officinalis L.) CV. 'Domaći oranž'. in: International Congress (III): Food Technology, Quality and Safety and XVII International Symposium: Feed Technology (FoodTech), Institute of Food Technology, Novi Sad, Serbia, from 25th to 27th Octobe, 525 - 530
Filipović, V., Ugrenović, V. (2020) Innovative approach in the production of valerian (Valeriana officinalis L.) using organic production methods. in: Subić Jonel, Jean Vasile Andrei, Jeločnik Marko [ed.] International Scientific Meeting: Sustainable agriculture and rural development in terms of the Republic of Serbia strategic goals realization within the Danube region: Science and practice in the ser, Belgrade: Institute of Agricultural Economics Belgrade, 593 - 611
Filipović, V., Ugrenović, V., Simić, I., Popović, S., Popović, V., Dragumilo, A., Ugrinović, M. (2020) The influence of application of organic bio-stimulant 'Zlatno inje' on the occurrence of lemon balm septoria leaf spot (Septoria melissae Desmazieres). in: Congress on Plant Protection (VIII): Integrated Plant Protection for Sustainable Crop Production and Forestry, Društvo za zaštitut bilja Srbije / Plant Protection Society of Serbia (PPSS), 25-29th Nov, (In print)
Filipović, V., Ugrenović, V., Glamočlija, Đ., Jevđović, R., Grbić, J., Sikora, V., Jaćimović, G. (2012) Efekti primene komercijalnih organskih đubriva na prinos i strukturu prinosa različitih sorti krompira. Selekcija i semenarstvo, vol. 18, br. 2, str. 71-90
Filipović, V., Aćimović, M., Ugrenović, V., Radanović, D., Marković, T., Popović, V., Matković, A. (2015) Valerian roots (Valeriana officinalis L.): Produced in autumn and spring planting date. Matières médicales / Lekovite sirovine, br. 35, str. 131-139
Frużyńska-Jóźwiak, D., Andrzejak, R. (2007) The incidence of diseases and pathogenic fungi on selected medicinal and spice plant in the regia of Poznań. Phytopathol. Pol., 46, 47-51
Gafni, A., Calderon, C.E., Harris, R., Buxdorf, K., Dafa-Berger, A., Zeilinger-Reichert, E., Levy, M. (2015) Biological control of the cucurbit powdery mildew pathogen Podosphaera xanthii by means of the epiphytic fungus Pseudozyma aphidis and parasitism as a mode of action. Frontiers in Plant Science, 6: 132-132
Garibaldi, A., Gilardi, G., Gullino, M.L. (2008) First Report of Powdery Mildew Caused by Podosphaera xanthii on Calendula officinalis in Italy. Plant Disease, 92(1): 174-174
Gesch, R.W. (2013) Growth and yield response of calendula (Calendula officinalis) to sowing date in the northern U.S. Industrial Crops and Products, 45: 248-252
Glamočlija, Đ., Janković, S., Popović, V., Filipović, V., Kuzevski, J., Ugrenović, V. (2015) Alternativne ratarske biljke u konvencionalnom i organskom sistemu gajenja. Beograd: Institut za primenu nauke u poljoprivredi, Monografija, 1-354
Gold, K.M., Townsend, P.A., Chlus, A., Herrmann, I., Couture, J.J., Larson, E.R., Gevens, A.J. (2020) Hyperspectral Measurements Enable Pre-Symptomatic Detection and Differentiation of Contrasting Physiological Effects of Late Blight and Early Blight in Potato. Remote Sensing, 12(2): 286-286
Goussous, S.J., Abu, E.F.M., Tahhan, R.A. (2010) Antifungal activity of several medicinal plants extracts against the early blight pathogen (Alternaria solani). Archives Of Phytopathology And Plant Protection, 43(17): 1745-1757
Helmy, K.G. (2016) Effect of Sodium Bicarbonate and Oils on Powdery Mildew of Matricaria chamomilla. Journal of Plant Protection and Pathology, 7(12): 861-866
Jevdović, R., Todorović, G., Kostić, M., Protić, R., Lekić, S., Zivanović, T., Sečanski, M. (2013) The effects of location and the application of different mineral fertilizers on seed yield and quality of pot marigold (Calendula officinalis L.). Turk. J. Field Crops, 18(1): 1-7
Jha, R., Regmi, R., Simon, L.S., Lal, A.A. (2014) Effect of Six plant extracts on the mycelial growth of Alternaria alternata causing leaf spot of Aloe vera. International Journal of research, 1(7): 1371-1373
Koné, S.B., Dionne, A., Tweddell, R.J., Antoun, H., Avis, T.J. (2010) Suppressive effect of nonaerated compost teas on foliar fungal pathogens of tomato. Biological Control, 52(2): 167-173
Król, B., Paszko, T. (2017) Harvest date as a factor affecting crop yield, oil content and fatty acid composition of the seeds of calendula (Calendula officinalis L.) cultivars. Industrial Crops and Products, 97: 242-251
Liu, X., Qiu, X., Duan, Z., Ping, D., Zhou, X., Yang, J., Wan, Y. (2018) A novel strain of Pseudozyma aphidis from mulberry parasitises the conidia of mulberry powdery mildew fungus Phyllactinia sp. and its biocontrol effect in the fields. Biocontrol Science and Technology, 28(1): 62-76
Machowicz-Stefaniak, Z., Zalewska, E., Zimowska, B. (2002) Fungi colonizing various organs of lemon balm (Melissa officinalis L.) cultivated in South-East Poland. in: Conf. EFPP (6th), Praha 2002, Plant Protect Sci 38, Proceedings, Special Issue 2, 347-350
Metz, N. (2017) Biologicals for the control of Alternaria solani under greenhouse and field conditions. in: Sixteenth Euroblight Workshop Aarhus, Denmark, pp. 14-17
Milenković, S. (2015) Dozvoljena sredstva za zaštitu bilja u organskoj poljoprivredi. Beograd: Nacionalno udruženje za razvoj organske proizvodnje Serbia Organika, 1-38
Miyamoto, T., Hayashi, K., Ogawara, T. (2020) First report of the occurrence of multiple resistance to Flutianil and Pyriofenone in field isolates of Podosphaera xanthii, the causal fungus of cucumber powdery mildew. European Journal of Plant Pathology, 156(3): 953-963
Pavlović, S., Stojanović, S., Kuzmanović, S., Starović, M., Živković, S., Dolovac, N. (2012) Etiology of diseases of some medicinal plants in plantation in Serbia. Zaštita bilja, vol. 63, br. 4, str. 224-241
Pavlović, S., Stojanović, S., Starović, M. (2009) The first report of Erysiphae cichoracearum var. cichoracearum on St. John's worth in Serbia. Zaštita bilja, vol. 60, br. 1, str. 11-17
Radovanović, N., Milutinović, M., Mihajlovski, K., Jović, J., Nastasijević, B., Rajilić-Stojanović, M., Dimitrijević-Branković, S. (2018) Biocontrol and plant stimulating potential of novel strain Bacillus sp. PPM3 isolated from marine sediment. Microbial pathogenesis, 120: 71-78
Ristić, D., Stanković, I., Vučurović, A., Berenji, J., Miličević, T., Krstić, B., Bulajić, A. (2011) Flower necrosis of Calendula officinalis L. caused by Alternaria alternata. in: Symposium Power of Fungi and Mycotoxins in Health and Disease, Primošten, Croatia, Abstracts, pp. 52
Skόrska, C., Golec, M., Mackiewicz, B., Góra, A., Dutkiewicz, J. (2005) Health effects of exposure to herb dust in valerian growing farmers. Ann. Agric. Environ. Med, 12: 247-252
Stat-Soft, Inc (2010) STATISTICA (Data Analysis Software System). USA, v.10.0 , (
Stepanović, M., Jevremović, S., Rekanović, E., Mihajlović, M., Milijašević-Marčić, S., Potočnik, I., Todorović, B. (2015) In vitro sensitivity of Alternaria solani to conventional fungicides and a biofungicide based on tea tree essential oil. Pesticidi i fitomedicina, vol. 30, br. 1, str. 25-33
Sun, J., Guo, L., Zang, W., Ping, W., Chi, D. (2008) Diversity and ecological distribution of endophytic fungi associated with medicinal plants. Science in China Series C: Life Sciences, 51(8): 751-759
Tanaka, K., Fukuda, M., Amaki, Y., Sakaguchi, T., Inai, K., Ishihara, A., Nakajima, H. (2017) Importance of prumycin produced by Bacillus amyloliquefaciens SD-32 in biocontrol against cucumber powdery mildew disease. Pest Management Science, 73(12): 2419-2428
Wiik, L., Rosenqvist, H., Liljeroth, E. (2018) Study on Biological and Economic Considerations in the Control of Potato Late Blight and Potato Tuber Blight. Journal of Horticulture, 05(01): 1-14
Yellareddygari, S.K.R., Taylor, R.J., Pasche, J.S., Zhang, A., Gudmestad, N.C. (2018) Predicting potato tuber yield loss due to early blight severity in the Midwestern United States. European Journal of Plant Pathology, 152(1): 71-79
Živković, S., Stevanović, M., Đurović, S., Ristić, D., Stošić, S. (2018) Antifungal activity of chitosan against Alternaria alternata and Colletotrichum gloeosporioides. Pesticidi i fitomedicina, vol. 33, br. 3-4, str. 197-204


article language: Serbian
document type: Original Scientific Paper
DOI: 10.5937/SelSem2001038F
published in SCIndeks: 26/07/2020
Creative Commons License 4.0