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Food and Feed Research
2021, vol. 48, br. 2, str. 175-184
Određivanje oksidacije proteina u hrani za ribe
Dragojlović Danka M.a, Popović Ljiljana M.b, Čakarević Jelena C.b, Spasevski Nedeljka J.a, Rakita Slađana M.a ORCID, Čolović Dušica S.a, Đuragić Olivera M.a ORCID
aInstitut za prehrambene tehnologije, Novi Sad, Srbija
bUniverzitet u Novom Sadu, Tehnološki fakultet, Srbija

e-adresa: danka.dragojlovic@fins.uns.ac.rs
Projekat:
Ministarstvo prosvete, nauke i tehnološkog razvoja Republike Srbije (institucija: Institut za prehrambene tehnologije, Novi Sad) (MPNTR - 451-03-68/2020-14/200222)

Ključne reči: ekstrakcija proteina; karbonilna jedinjenja; globulini; 2,4-dinitrofenilhidrazin
Sažetak
Cilj ovog istraživanja bilo je razvijanje pouzdane, jednostavnije, jeftinije metode za merenje oksidacije proteina u složenim uzorcima, kao što je hrana za ribe, koja u svom sastavu sadrži različite izvore proteina. U tu svrhu korišćena je modifikovana metoda za kvantifikaciju proteinskih karbonila, koja je zasnovana na 2,4dinitrofenilhidrazin (DNPH)-metodi. Modifikacija metode se sastojala u promeni rastvarača (destilovana voda različite koncentracije rastvora NaCl i KCl) i vremenu ekstrakcije (nakon homogenizacije i preko noći), kao i koncentracije trihlor sirćetne kiseline (TCA, 10 i 25%) za precipitaciju proteina. Uočeno je da produženo vreme ekstrakcije (tokom noći), veća koncetracija TCA (25%) i 0,5 M rastvor KCl-a dovodi do povećanja koncentracije proteina. S druge strane, najmanja količina proteina dobijena je ekstrakcijom pomoću destilovane vode. U pogledu količine proteinskih karbonila, pokazano je da je najveći sadržaj postignut ekstrakcijom pomoću 0,15 M i 0,5 M KCl. Tokom skladištenja uzoraka, pod ekstremnim uslovima, uočeno je da se količina karbonilnih jedinjenja povećava. A u poređenju sa originalnom metodom, modifikovanom metodom za merenje oksidacije proteina izmerena je veća količina karbonilnih jedinjenja u svakoj fazi skladištenja.
Reference

Armenteros, M., Heinonen, M., Ollilainen, V., Toldrá, F., Estevez, M. (2009) Analysis of Protein Carbonyls in Meat Products by Using the DNPH Method, Fluorescence Spectroscopy and Liquid Chromatography-electrospray Ionisation-mass Spectrometry (LC-ESI-MS). Meat Science, 83(1): 104-112

Boyd, C.E., Polioudakis, M. (2006) Land Use for Aquaculture Production. Retrieved from Global Aquaculture Advocate. https://www.aquaculturealliance.org/advocate/landuse-for-aquaculture-production

Chen, N., Zhao, M., Chassenieux, C., Nicolai, T. (2016) Data on the Characterization of Native Soy Globulin by SDS-Page, Light Scattering and Titration. Data in Brief, 9: 749-752

Chookird, D., Tantikitti, C., Pongdara, A., Srichanun, M. (2010) Effect of Hemoglobin Powder Substituted for Fishmeal on Growth Performance, Protein Digestibility, and Trypsin Gene Expression in Litopenaeusvannamei. Songklanakarin Journal of Science & Technology, 32(2): 119-127, http://rdo.psu.ac.th/sjst/journal/32-2/0125-3395-32-2-119-127.pdf

Davis, D.A. (2015) Feed and Feeding Practices in Aquaculture. Cambridge, UK: Woodhead Publishing, 1st ed., 128-150

Decker, E.A., Faustman, C., Lopez-Bote, C.J. (2000) Antioxidants in Muscle Foods: Nutritional Strategies to Improve Quality. New York: John Wiley & Sons, 1st edition

Estévez, M., Ventanas, S., Heinonen, M. (2011) Formation of Strecker Aldehydes Between Protein Carbonyls-a-Aminoadipic and g-glutamic Semialdehydes-and Leucine and Isoleucine. Food Chemistry, 128(4): 1051-1057

Eymard, S., Baron, C.P., Jacobsen, C. (2009) Oxidation of Lipid and Protein in Horse Mackerel (Trachurustrachurus) Mince and Washed Minces During Processing and Storage. Food Chemistry, 114(1): 57-65

Food and Agricultural Organization (2012) State of Food and Agriculture 2012: Investing in Agriculture for a Better Future. https://www.fao.org/publications/sofa/2012/en

Guyon, C., Meynier, A., de Lamballerie, M. (2016) Protein and Lipid Oxidation in Meat: A Review with Emphasis on High-Pressure Treatments. Trends in Food Science & Technology, 50: 131-143

Hu, X.Z., Cheng, Y.Q., Fan, J.F., Lu, Z.H., Yamaki, K., Li, L.T. (2010) Effects of Drying Method on Physicochemical and Functional Properties of Soy Protein Isolates. Journal of Food Processing and Preservation, 34(3): 520-540

Huntington, T.C., Hasan, M.R. (2009) Fish as Feed Inputs for Aquaculture-Practices, Sustainability and Implications: A Global Synthesis. FAO Fisheries and Aquaculture Technical Paper, 518: 1-61, http://www.fao.org/3/i1140e/i1140e01.pdf

Jin, D.X., Liu, X.L., Zheng, X.Q., Wang, X.J., He, J.F. (2016) Preparation of Antioxidative Corn Protein Hydrolysates, Purification and Evaluation of Three Novel Corn Antioxidant Peptides. Food Chemistry, 204: 427-436

Jobling, M. (2012) National Research Council (NRC): Nutrient requirements of fish and shrimp. Aquaculture International, 20: 601-602

Laemmli, U.K. (1970) Cleavage of Structural Proteins During the Assembly of Head of Bacteriophage T4. Nature, 227: 680-685

Levine, R.L., Garland, D., Oliver, C.N., Amici, A., Climent, I., Lenz, A., Ahn, B., Shaltiel, S., Stadtman, E.R. (1990) Determination of Carbonyl Content in Oxidatively Modified Proteins. Methods Enzymology, 186: 464-478

Levine, R.L., Stadtman, E.R. (2001) Oxidative Modification of Proteins During Aging. Experimental Gerontology, 36(9): 1495-1502

Levine, R.L., Williams, J.A., Stadtman, E.P., Shacter, E. (1994) Carbonyl Assays for Determination of Oxidatively Modified Proteins. Methods in Enzymology, 233: 346-357

Lowry, O., Rosenbrough, N., Fair, A., Randall, R. (1951) Protein Measurement with the Folin Phenol Reagent. Journal of Biological Chemistry, 193: 265-275, https://www.cabdirect.org/cabdirect/abstract/19511404458

Lund, M.N., Lametsch, R., Hviid, M.S., Jensen, O.N., Skibsted, L.H. (2007) High-Oxygen Packaging Atmosphere Influences Protein Oxidation and Tenderness of Porcine Longissimus Dorsi During Chill Storage. Meat Science, 77(3): 295-303

Maehre, H.K., Dalheim, L., Edvinsen, G.K., Elvevoll, E.O., Jensen, I.J. (2018) Protein Determinationmethod Matters. Foods, 7(1): 5-5

Mandal, S., Mandal, R.K. (2000) Seed Storage Proteins and Approaches for Improvement of Their Nutritional Quality by Genetic Engineering. Current Science, 79(5): 576-587, https://www.jstor.org/stable/24105073

Mitra, B., Lametsch, R., Akcan, T., Ruiz-Carrascal, J. (2018) Pork Proteins Oxidative Modifications Under the Influence of Varied Time-temperature Thermal Treatments: A Chemical and Redox Proteomics Assessment. Meat Science, 140: 134-144

Papastergiadis, A., Mubiru, E., van Langenhove, H., de Meulenaer, B. (2012) Malondialdehyde Measurement in Oxidized Foods: Evaluation of the Spectrophotometric Thiobarbituric Acid Reactive Substances (TBARS) Test in Various Food. Journal of Agriculture and Food Chemistry, 60: 9589-9594

Phillips, G.O., Williams, P.A. (2011) Handbook of Food Proteins. Cambridge, UK: Woodhead Publishing

Popović, Lj., Stolić, Ž., Čakarević, J., Torbica, A., Tomić, J., Šijački, M. (2017) Biologically Active Digests from Pumpkin Oil Cake Protein: Effect of Crosslinking by Transglutaminase. Journal of the American Oil Chemists Society, 94: 1245-1251

Porzio, M.A., Pearson, A.M. (1977) Improved Resolution of Myofibrillar Proteins with Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis. Biochimica et Biophysica Acta (BBA): Protein Structure, 490(1): 27-34

Rakita, S., Čolović, D., Levart, A., Banjac, V., Čolović, R., Dragojlović, D., Đuragić, O. (2020) A Rapid Spectrophotometric Method for Determination of Thiobarbituric Acid Reactive Substances in Rainbow Trout Feed. Food and Feed Research, 47(1): 43-54, http://scindeks.ceon.rs/article.aspx?artid=2217-53692001043R

Recreational Fisheries FAO (2012) Technical Guidelines for Responsible Fisheries 13. Food and Agriculture Organization of the United Nations. Rome, http://www.fao.org/docrep/016/i2708e/i2708e00.htm

Reeg, S., Grune, T. (2015) Protein Oxidation in Aging: Does It Play a Role in Aging Progression?. Antioxidants & Redox Signaling, 23(3): 239-255

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Requena, J.R., Levine, R.L., Stadtman, E.R. (2003) Recent Advances in the Analysis of Oxidized Proteins. Amino Acids, 25(3-4): 221-226

Soglia, F., Petracci, M., Ertbjerg, P. (2016) Novel DNPH-Based Method for Determination of Protein Carbonylation in Muscle and Meat. Food Chemistry, 197: 670-675

Stadman, E.R., Levine, R.L. (2000) Protein Oxidation. Annals New York Academy of Sciences, 899(1): 191-208

Traore, S., Aubry, L., Gatellier, P., Przybylski, W., Jaworska, D., Kajak-Siemaszko, K., Santé-Lhoutellier, V. (2012) Effect of Heat Treatment on Protein Oxidation in Pig Meat. Meat Science, 91(1): 14-21

Weingärtner, H., Cabrele, C., Herrmann, C. (2012) How Ionic Liquids Can Help to Stabilize Native Proteins. Physical Chemistry Chemical Physics, 14(2): 415-426

Yadi, F.Y., Rosentrater, K.A., Muthukumarappan, K. (2012) Alternative Protein Sources for Aquaculture Feeds. Journal of Aquaculture Feed Science and Nutrition, 4(1): 1-26
   

O članku

jezik rada: engleski
vrsta rada: izvorni naučni članak
DOI: 10.5937/ffr48-34712
primljen: 02.11.2021.
revidiran: 02.12.2021.
prihvaćen: 08.12.2021.
objavljen onlajn: 15.12.2021.
objavljen u SCIndeksu: 25.03.2022.
metod recenzije: jednostruko anoniman
Creative Commons License 4.0

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