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2016, vol. 35, br. 3, str. 337-346
Analiza genetičke varijabilnosti Epstajn-Bar virusa kod infektivne mononukleoze - ispitivanje potencijalne korelacije sa biohemijskim parametrima infekcije jetre
aUniverzitet u Beogradu, Medicinski fakultet, Institut za mikrobiologiju i imunologiju
bKlinički centar Srbije, Klinika za infektivne i tropske bolesti, Beograd + Univerzitet u Beogradu, Medicinski fakultet
cUniverzitet u Beogradu, Medicinski fakultet, Institut za medicinsku statistiku i informatiku
dKlinički centar Srbije, Centar za radiologiju i magnetnu rezonancu, Beograd
eKlinički centar Srbije, Klinika za ORL i MFH, Beograd + Univerzitet u Beogradu, Medicinski fakultet

e-adresaana.banko@mfub.bg.ac.rs
Projekat:
Medicinski značaj genetičke varijabilnosti virusa (MPNTR - 175073)

Ključne reči: EBNA; EBV; genski polimorfizam; infektivna mononukleoza; LM P1; transaminaze
Sažetak
Uvod: Primarna infekcija koju izaziva Epštajn-Bar virus obicno je asimptomatska. Povremeno, ova infekcija može se manifestovati benignim limfoproliferativnim oboljenjem, infektivnom mononukleozom (IM), za čije vreme polovina pacijenata dobije hepatitis. Ciljevi ove studije bili su evaluacija EBV genskih polimorfizama koji su zastupljeni u IM izoIatima ovog geografskog područja, kao i ispitivanje korelacije specifične virusne varijabilnosti sa dostupnim jetrinim biohemijskim parametrima IM pacijenata. Metode: Studija je uključila uzorke plazme od 128 IM pacijenata. Za umnožavanje EBNA2, LM P1 i EBNA1 gena korišćen je nested-PCR metod. EBNA2 genotipizacija izvršena je vizuelizacijom PCR produkata korišćenjem gel elektroforeze. LMP1 i EBNA1 ispitivanje obuhvatilo je analizu njihovih genskih sekvenci, a zatim i filogenetsku i statisticku obradu. Rezultati: Prisustvo EBV DNK u uzorcima plazme koreli - salo je sa neophodnošću bolničkog lečenja pacijenata (p=0,034). Većina izolata pripadala je genotipu 1. Analizom LMP1 varijabilnosti dobijene su 4 poznate varijante i 2 nove delecije (27-bp i 147-bp). Među 3 ispitivane karakteristike LMP1 izolata, broj 33-bp ponovaka manji od referenta 4,5 jedini je bio apsolutno povezan sa povišenim nivoom aspartat aminotransferaze (AST) i alanin transaminaze (ALT). EBNA1 varijabilnost dala je samo prototipne subtipove. Specifične kombinacije EBNA2, LM P1 i EBNA1 polimorfizama, LM P1 sa delecijom/P-thr i LM P1 bez delecije/P-ala, kao i genotip 1/<4.5 33-bp LM P1 ponovka ili genotip 2/<4.5 33-bp LM P1 ponovka, pokazale su korelaciju sa povišenim vrednostima transaminaza. Zaključak: Ovo je prva studija u kojoj je identifikovana povezanost između EBV genetičke varijabilnosti i biohemijskih parametara IM pacijenata. Prikazani rezultati pokazuju mogućnost identifikovanja dijagnostičkih EBV markera povezanih sa statusom oštećenja jetre.
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