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Medicinski pregled
2015, vol. 68, br. 3-4, str. 85-92
jezik rada: engleski
vrsta rada: izvorni naučni članak
doi:10.2298/mpns1504085g


Imunohistohemijska ekspresija p16INK4a u inflamacijskim, preneoplastičnim i neoplastičnim lezijama grlića materice
aClinical Center Banja Luka, University of Banja Luka, Faculty of Medicine, the Republic of Srpska, Bosnia and Herzegovina
bGeneral Hospital, Kotor Varoš, the Republic of Srpska, Bosnia and Herzegovina
cUniversity of Banja Luka, Faculty of Medicine, the Republic of Srpska

e-adresa: radoslavgajanin@gmail.com

Sažetak

Uvod. Humani papiloma virusi visokog rizika imaju glavnu ulogu u nastanku displazije i karcinoma cerviksa. p16INK4a se može smatrati 'surogat' markerom prisustva aktivne infekcije humanim papiloma virusima visokog rizika u displastičnim i neoplastičnim ćelijama grlića materice. Cilj istraživanja bio je utvrđivanje prisustva i stepena ekspresije p16INK4a u inflamacijskim, preneoplastičnim i neoplastičnim lezijama grlića materice. Materijal i metode. Istraživanje je izvršeno na 109 bioptičkih uzoraka grlića materice. Kod 36 pacijentkinja dijagnostikovan je karcinom grlića materice; kod 34 pacijentkinje je utvrđena preneoplastična promena (displazija) u pločastosojevitom epitelu grlića materice; a kod 39 utvrđen je nespecifični inflamacijski proces. Na svim uzorcima urađena je imunohistohemijska analiza upotrebom antitela na p16INK4a. Rezultati. Ekspresija p16INK4a je verfikovana u svim slučajevima kod pacijentkinja sa karcinomom cerviksa (100%); u 67,65% slučajeva u displastičnim lezijama grlića materice i 38,5% slučajeva u inflamacijskim lezijama. Statistički visoko značajna razlika je prisutna u prisustvu i stepenu ekspresije između neoplastičnih, displastičnih i inflamacijskih lezija grlića materice (χ² = 76,02, p < 0,001). Ekspresija je češća i visokog je stepena u neoplastičnim i displastičnim lezijama, u odnosu na inflamacijske lezije i displazije niskog stepena. Zaključak. Analizom prisustva p16INK4a može se diferencirati neneoplastična promena od preneoplastičnih promena visokog stepena i neoplastičnih promena na grliću materice. Upotrebom p16INK4a u interpretaciji graničnih lezija na cerviksu omogućava se racionalan terapijski tretman pacijentkinja.

Ključne reči

Skvamozne intraepitelijalne lezije cerviksa; Karcinomigrlića materice; Cervicitis; Imunohistohemija; P16INK4a; Papilomaviride; Infekcije papiloma virusom; Biološki markeri

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