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Vojnosanitetski pregled
2019, vol. 76, iss. 2, pp. 161-167
article language: English
document type: Original Scientific Paper
doi:10.2298/VSP170125075B

Creative Commons License 4.0
Influence of the infiltrate density in the interstitium on the prognosis of primary glomerulonephritis
aClinical Center of Vojvodina, Clinic for Nephrology and Clinical Immunology, Novi Sad + University of Novi Sad, Faculty of Medicine, Novi Sad
bClinical Center of Vojvodina, Clinic for Nephrology and Clinical Immunology, Novi Sad

e-mail: dusan.bozic@mf.uns.ac.rs

Abstract

Background/Aim. Development of inflammatory changes, fibrosis and loss of morphological structures of the interstitium have an important role in pathogenesis of primary glomerulonephritis, affecting the development, course and prognosis of the disease. The aim of this study was to determine the influence of changes in the interstitium on the prognosis of primary glomerulonephritis. Methods. The research included 216 patients suffering from different types of primary glumeronephritis treated at the Clinic for Nephrology and Clinical Immunology of the Clinical Center of Vojvodina, Serbia who were being monitored on average for 77.5 months. After determining on pathohistological diagnosis of the type of glomerulonephritis, renal changes in the interstitium were quantified. Numerical density in the tissue volume unit and structure of infiltrates of the interstitium were established by using the Weibel system (M42) incorporated into light microscope. Routine analyses were performed by using standard laboratory procedure. Results. During the research period the highest numerical density of infiltrates was verified in extracapillary glomerulonephritis (147,869 × mm-3), slightly less in membranoproliferative glomerulonephritis (116,800 × mm-3) and focal segmental glomerulosclerosis (96,147 × mm-3), and the least being in glomerulonephritis with minimal changes (11,416 × mm-3). In all types of glomerulonephritis, apart from glomerulonephritis with minimal changes, there was a significantly (p < 0.0005) higher numerical density and incidence of infiltrate cells in relation to the control group. By comparing the numerical density of infiltrates of all cells to the parameters of renal function, a significant (p < 0.01) correlation of these phenomena was established. In order to get a better insight into the speed of progression of renal failure by setting a numerical limit of the density of infiltrates < 100,000 / > 100,000 cells/mm3, regardless of the type of glomerulonephritis, a prognostic predictor was established on the basis of which the patients with lower infiltration of the interstitium had significantly (p < 0.005) lower progression of renal failure. Conclusion. Density of infiltrates in the interstitium in primary glomerulonephritis is an important early prognostic predictor of progression of renal failure.

Keywords

References

Abercrombie, M. (1946) Estimation of nuclear population from microtome sections. Anatomical Record, 94(2): 239-247
Alexopoulos, E., Seron, D., Hartley, R. B., Nolasco, F., Cameron, J. S. (1989) The Role of Interstitial Infiltrates in IgA Nephropathy: A Study with Monoclonal Antibodies. Nephrology Dialysis Transplantation, 4(3): 187-195
Bob, F., Gluhovschi, G., Herman, D., Petrica, L., Bozdog, G., Gluhovschi, C., Velciov, S., Gadalean, F., Timar, R., Potencz, E., Dema, A., Schiller, A. (2014) Immunohistochemical study of tubular epithelial cells and vascular endothelial cells in glomerulonephritis. Renal Failure, 36(8): 1208-1214
Bob, R.F., Herman, D., Gluhovschi, G., Petrica, L., Bozdog, G., Velciov, S., i dr. (2011) Is The histological scoring system useful in assessing patients with glomerulonephritis?. BANTAO J, 9(2), 72-76
Bohle, A., Christ, H., Grund, K. E., Mackensen, S. (1979) The Role of the Interstitium of the Renal Cortex in Renal Disease. Contrib Nephrol, : 109-114
Burton, C.J., Walls, J., Harris, K.P. (1995) Characterisation of the serum factor that stimulates human cortical epithelial cells to produce fibronectin (FN) and PDGF (abstract). J Am Soc Nephrol, 6: 1010
Chatenoud, L., Bach, M. (1981) Abnormalities of T-cell subsets in glomerulonephritis and systemic lupus erythematosus. Kidney International, 20(2): 267-274
Churg, J., Duffy, J.L. (1973) Classification of glomerulonephritis based on morphology. Perspect Nephrol Hypertens, 1 Pt 1: 43-61
Danilewicz, M., Wagrowska-Danilewicz, M. (1995) Idiopathic membranous glomerulonephritis: a quantitative study of glomerular and interstitial lesions. Pol J Pathol, 46(3): 173-7
Habib, R. (1973) Classification of glomerulonephritis based on morphology. Perspect Nephrol Hypertens, 1 Pt 1: 17-41
Han, Y., Ma, F.Y., Tesch, G.H., Manthey, C.L., Nikolic-Paterson, D.J. (2013) Role of macrophages in the fibrotic phase of rat crescentic glomerulonephritis. American Journal of Physiology-Renal Physiology, 304(8): F1043-F1053
Hancock, W.W., Becker, G.J., Atkins, R.C. (1982) A Comparison of Fixatives and Immunohistochemical Technics for Use with Monoclonal Antibodies to Cell Surface Antigens. American Journal of Clinical Pathology, 78(6): 825-831
Hao, W., Rovin, B.H., Friedman, A. (2014) Mathematical model of renal interstitial fibrosis. Proceedings of the National Academy of Sciences, 111(39): 14193-14198
Hooke, D.H., Gee, D.C., Atkins, R.C. (1987) Leukocyte analysis using monoclonal antibodies in human glomerulonephritis. Kidney International, 31(4): 964-972
Hu, S., Jia, X., Li, J., Zheng, X., Ao, J., Liu, G., Cui, Z., Zhao, M. (2016) T cell infiltration is associated with kidney injury in patients with anti-glomerular basement membrane disease. Science China Life Sciences, 59(12): 1282-1289
Ihm, C. (2015) Hypertension in Chronic Glomerulonephritis. Electrolytes & Blood Pressure, 13(2): 41
Lan, H.Y., Paterson, D.J., Atkins, R.C. (1991) Initiation and evolution of interstitial leukocytic infiltration in experimental glomerulonephritis. Kidney International, 40(3): 425-433
Li, H., Hancock, W.W., Hooke, D.H., Dowling, J.P., Atkins, R.C. (1990) Mononuclear cell activation and decreased renal function in IgA nephropathy with crescents. Kidney International, 37(6): 1552-1556
Lin, T., Yang, S., Hua, K., Tsai, Y., Lin, S., Ka, S. (2016) SPAK plays a pathogenic role in IgA nephropathy through the activation of NF-κB/MAPKs signaling pathway. Free Radical Biology and Medicine, 99: 214-224
Nikolic-Paterson, D.J., Wang, S., Lan, H.Y. (2014) Macrophages promote renal fibrosis through direct and indirect mechanisms. Kidney International Supplements, 4(1): 34-38
Platt, J. L. (1983) Immune cell populations in cutaneous delayed-type hypersensitivity. Journal of Experimental Medicine, 158(4): 1227-1242
Schena, F. P., Mastrolitti, G., Jirillo, E., Munno, I., Pellegrino, N., Fracasso, A. R., Aventaggiato, L. (1989) Increased production of interleukin-2 and IL-2 receptor in primary IgA nephropathy. Kidney International, 35(3): 875-879
Seron, D., Alexopoulos, E., Raftery, M. J., Hartley, B., Cameron, J. S. (1990) Number of Interstitial Capillary Cross-Sections Assessed by Monoclonal Antibodies: Relation to Interstitial Damage. Nephrology Dialysis Transplantation, 5(10): 889-893
Stachura, I., Si, L., Madan, E., Whiteside, T. (1984) Mononuclear cell subsets in human renal disease: Enumeration in tissue sections with monoclonal antibodies. Clin Immunol Immunopathol, 30(3): 362-73
Truong, L.D., Trostel, J., McMahan, R., Chen, J., Garcia, G.E. (2016) Macrophage A2A Adenosine Receptors Are Essential to Protect from Progressive Kidney Injury. American Journal of Pathology, 186(10): 2601-2613
Weibel, E.R. (1982) Stereology-a bridge between morphology and phisiology. Acta Stereologica, 1:23-31
Weibel, E.R., Gomez, D.M. (1962) A principle for counting tissue structures on random sections. Journal of Applied Physiology, 17(2): 343-348