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Food and Feed Research
2018, vol. 45, br. 1, str. 67-76
jezik rada: engleski
vrsta rada: originalan članak
objavljeno: 13/07/2018
doi: 10.5937/FFR1801067M
Creative Commons License 4.0
Toksin genotipizacija sojeva Clostridium perfringens izolovanih iz hrane za životinje i njihov značaj u etiologiji enterotoksemija domaćih životinja
aNaučni institut za veterinarstvo 'Novi Sad', Novi Sad
bUniverzitet u Beogradu, Fakultet veterinarske medicine
cInstitut za prehrambene tehnologije, Novi Sad

e-adresa: dubravka@niv.ns.ac.rs

Projekat

Istraživanje farmakoloških karakteristika antimikrobnih agenasa, uvođenje novih tehnoloških rešenja i alternativnih metoda profilakse s ciljem da se poboljša kontrola infektivnih oboljenja domaćih životinja (MPNTR - 31071)
Molekularno-genetička i ekofiziološka istraživanja u zaštiti autohtonih animalnih genetičkih resursa, očuvanju dobrobiti, zdravlja i reprodukcije gajenih životinja i proizvodnji bezbedne hrane (MPNTR - 46002)

Sažetak

Clostridium perfringens je Gram-pozitivna, anaerobna, sporulišuća, štapićasta bakterija, ubikvitarno rasprostranjena u prirodi. Kod različitih sojeva C. Perfringens, do danas, identifikovano je oko 17 vrsta toksina. C. perfringens je uzročni agens različitih oboljenja (sindroma), ali su crevne infekcije/intoksikacije najčešće i od najvećeg značaja za zdravlje farmski gajenih životinja. U ovom radu prikazujemo rezultate ispitivanja sojeva C. perfringens poreklom iz hrane za životinje (n=34) i jednog izolata iz organa krave uginule sa znacima enterotoksemije na prisustvo gena: cpa, cpb, cpb2, cpe, etx i iap primenom multipleks PCR tehnike. Svi sojevi C. perfringens izolovani iz hrane za životinje, identifikovani su kao tip A koji poseduje samo cpa gen (n=21) ili tip A koji produkuje b2-toksin, odnosno ima cpa i cpb2 gene (n=13). U radu diskutujemo o ulozi alfa (α) i beta-2 (β2) toksina u patogenezi enterotoksemija domaćih životinja, kao i aktuelnom zakonskom propisu po kojem ova vrsta bakterije ne sme biti prisutna u hrani za životinje. Primena PCR tehnike u svakodnevnoj praksi omogućila bi toksin-genotipizaciju sojeva C. perfringens, a time i realne osnove za uspostavljanje graničnih dozvoljenih vrednosti za ovu vrstu bakterije u hrani za životinje.

Ključne reči

Clostridium perfringens; toksin genotipizacija; hrana za životinje; multipleks PCR tehnika; cpa gen; cpb2 gen

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