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Food and Feed Research
2016, vol. 43, br. 1, str. 19-24
jezik rada: engleski
vrsta rada: originalan članak
doi:10.5937/FFR1601019T

Creative Commons License 4.0
Uticaj faktora sredine na formiranje biofilma Listeria monocytogenes
aUniverzitet u Novom Sadu, Tehnološki fakultet
bInstitut za prehrambene tehnologije, Novi Sad

e-adresa: ruzica.tomicic@uns.ac.rs

Projekat

Istraživanje savremenih biotehnoloških postupaka u proizvodnji hrane za životinje u cilju povećanja konkurentnosti, kvaliteta i bezbednosti hrane (MPNTR - 46012)

Sažetak

Listeria monocytogenes je sveprisutna u prirodi i veliki problem u prehrambenoj industriji, s obzirom da je uzročnik listerioze, ozbiljne bolesti izazvane hranom. Postoje mnogi putevi kojim se ovaj mikroorganizam može uneti u fabričko okruženje. Sposobnost razmnožavanja ovog mikroorganizma na temperaturi skladištenja (+4 °C) predstavlja veliki problem u prehrambenoj industriji. Biofilmovi se smatraju važnim u smislu opstanka i razvoja mikroorganizama koji ih štite od delovanja sredstava za dezinfekciju. Cilj ovog rada je bio da se ispita sposobnost formiranja biofilma osam sojeva L. monocytogenes izolovanih iz mesa i jednog referentnog soja L. monocytogenes ATCC 19111 u dva različita medija Tryptone soya yeast extract broth (TSYEB) i Brain-heart infusion broth (BHI) na temperaturama 7 °C, 25 °C, 37 °C, 42 °C tokom 5 dana. Metod korišćen za procenu sposobnosti formiranja biofilma je kristal violet test. Rezultati istraživanja su pokazali da je najmanja sposobnost formiranja biofilma sojeva L. monocytogenes primećena na temperaturi 7 °C. Pored toga, najefikasniji medijum koji je uticao na formiranje biofilma sojeva izolovanih iz mesa je BHI dok je za referentni soj TSYEB.

Ključne reči

formiranje biofilma; Listeria monocytogenes; medijum; temperatura

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