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2014, vol. 65, br. 2, str. 77-84
Morfološka i molekularna identifikacija Cercospora apii na celeru u Srbiji
aInstitut za zaštitu bilja i životnu sredinu, Beograd
bUniverzitet u Beogradu, Poljoprivredni fakultet

e-adresaanjamilosavljevic@yahoo.com
Projekat:
Razrada integrisanog upravljanja i primene savremenih principa suzbijanja štetnih organizama u zaštiti bilja (MPNTR - 31018)

Ključne reči: Cercospora apii; Apium graveolens var. dulce; identifikacija
Sažetak
C. apii predstavlja jednu od najštetnijih fitopatogenih gljiva celera. Tokom 2012. god na teritoriji Velikog Gradišta su prikupljeni uzorci celera sa karakterističnim simptomima pegavosti lista i njihova identifikacija je izvršena pomoću morfoloških i molekularnih metoda. Test patogenosti je obavljen na zdravim biljkama i svi ispitivani izolati prouzrokuju karakteristične simptome nakon inokulacije. Konidiofore iz stroma formiraju zbijene fascikule ili su pojedinačne, prave do blago zakrivljene, smeđe boje. Konidije su pojedinačne, hijalinske i septirane (5-13 septi), cilindrične do blago sužene na jednoj strani ili prave do blago zakrivljene i zatupaste na vrhu. U osnovi su zasečene, skoro kvadratnog oblika i stanjene. Amplifikovani su fragmenti očekivanih dužina za internal transcribed spacer (ITS1/ITS4), kalmodulin (CAL-228F/CAL2Rd) i histon (CYLH3F/CYLH3R) genima i dobijene sekvence su pokazale 100% identitet sa sekvencama C. apii u bazi gena.
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O članku

jezik rada: srpski
vrsta rada: naučni članak
DOI: 10.5937/zasbilj1402077M
objavljen u SCIndeksu: 21.05.2015.

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