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2019, vol. 58, br. 4, str. 5-11
Imunohistohemijska analiza receptora 1 za vaskularni faktor rasta kod T1 karcinoma mokraćne bešike visokog gradusa i konkomitantnim in situ karcinomom
aUniverzitet u Nišu, Medicinski fakultet, Katedra Patologija
bUniverzitet u Nišu, Medicinski fakultet, Katedra Farmakologija sa toksikologijom
cUniverzitet u Nišu, Medicinski fakultet, Katedra Farmakologija sa toksikologijom + Klinički centar Niš, Klinika za onkologiju

e-adresaanav.ristic@gmail.com
Projekat:
Etiologija, dijagnostika, prevencija, i terapija endemske nefropatije i sa njom povezanih tumora urotela - značaj istraživanja genoma i proteoma (MPNTR - 175092)

Ključne reči: angiogeneza; VEGFR1; karcinom mokraćne bešike; carcinoma in situ
Sažetak
Receptor 1 za vaskularni faktor rasta (VEGFR1) smanjuje angiogenetsku aktivnost vaskularnog faktora rasta (VEGF), ponašajući se kao lažni receptor za VEGF i ograničavajući njegovu dostupnost za prave angiogene receptore. Cilj rada je da se ustanovi značaj VEGFR1 ekspresije kod T1 karcinoma mokraćne bešike visokog gradusa (HGT1) sa konkomitantnim in situ karcinomom (CIS) i da se odredi imunohistohemijski marker koristan za praćenje "nepredvidivih" HGT1 bolesnika. Analizirano je 137 HGT1 karcinoma mokraćne bešike. Konkomitantni CIS dijagnostikovan je kod 21 bolesnika (15,33%). 137 parafinskih uzoraka uklopljeno je u tkivne mikroareje i obojeno zečjim monoklonalnim antitelom na VEGFR1 (N-term: Y103/Epitomics, razblaženje 1 : 250). Imunohistohemijska reakcija procenjivana je na sledeći način: negativna ekspresija ukoliko je obojeno ≤ 10% ćelija, a pozitivna ukoliko je obojeno > 10% ćelija. Procenjivana je i membranska i citoplazmatska ekspresija i skorirana prema skali od 0 do 3 (0 - nema bojenja; 1 - slabo bojenje; 2 - umereno; i 3 - intenzivno). Nakon praćenja u trajanju od 50 meseci, utvrđeno je da bolesnici sa HGT1 karcinomom mokraćne bešike i konkomitantnim CIS imaju ukupno manju stopu preživljavanja (p < 0,05), kao i manju ekspresiju VEGFR1 (p < 0,05). Bolesnici sa pozitivnim VEGFR1 imali su duži vremenski period bez recidiva (p < 0,01) i duže ukupno preživljavanje (p < 0,01). Istraživanje je pokazalo da procena VEGFR1 ekspresije može biti dijagnostička dopuna za odabir bolesnika sa HGT1 urotelnim karcinomom mokraćne bešike, kojima je neophodno intenzivnije praćenje, posebno ukoliko je prisutan i CIS.
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O članku

jezik rada: engleski
vrsta rada: izvorni naučni članak
DOI: 10.5633/amm.2019.0401_NE_RADI
objavljen u SCIndeksu: 24.02.2020.
metod recenzije: dvostruko anoniman
Creative Commons License 4.0