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Veterinarski glasnik
2016, vol. 70, iss. 3-4, pp. 121-129
article language: English
document type: Original Paper
published on: 23/12/2016
doi: 10.2298/VETGL1604121N
Wine polyphenol resveratrol inhibits contractions of isolated rat uterus by activation of smooth muscle inwardly rectifying potassium channels
aUniversity of Belgrade, Faculty of Medicine, Institute of Pharmacology
bClinical Center of Serbia, Clinic for Gynecology and Obstetrics, Belgrade
cUniversity of Belgrade, Faculty of Agriculture, Department of Food Technology and Biochemistry
dUniversity of Belgrade, Faculty of Pharmacy, Department of Pharmaceutical Chemistry and Drug Analysis
eUniversity of Belgrade, Faculty of Veterinary Medicine, Department of Pharmacology and Toxicology
fSerbian Academy of Sciences and Arts - SASA, Belgrade
gInstitute of Physiology, University of Tuebingen, Germany

Project

Development of new technology for production of red wine and diatery supplements reach with polyphenols with cardioprotective effects (MESTD - 31020)

Abstract

Resveratrol is a phytoalexin produced in a number of plant species including grapes. The benefit of resveratrol to health is widely reported. Resveratrol has been found to promote relaxation of non-pregnant and pregnant uterus, but its mechanism of action is unclear. The aims of our study were to investigate the involvement of inwardly rectifying potassium channels (Kir) in inhibitory effects of resveratrol on three models of contractions of non-pregnant rat uterus: the spontaneous rhythmic contractions (SRC), oxytocin-elicited phasic contractions and tonic oxytocin-elicited contractions. Uterine strips were obtained from virgin female Wistar rats in oestrus. Strips were mounted into organ bath for recording isometric tension in Krebs-Ringer solution. Experiments followed a multiple curve design. In order to test the involvement of Kirchannels in a mechanism of action of resveratrol (1-100 μM),BaCl2 (1 mM),a antagonist of inwardly rectifying potassium channels was used. Resveratrol induced a concentration-dependent relaxation of all models of contractions. BaCl2 antagonized the response to resveratrolon SRC and oxytocin-elicited phasic contractions. Relaxation achieved by resveratrolon tonic oxytocin-elicited concentrations was insensitive to BaCl2.The antagonism of resveratrol effects by inwardly rectifying potassium channels antagonist suggests that Kir channels are involved in resveratrol action on phasic contractions of rat uterus. Inhibitory effect of resveratrol on tonic contractions did not include Kir channels.

Keywords

resveratrrol; uterus; inwardly recifying potassium channels; Kir