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Journal on Processing and Energy in Agriculture
2018, vol. 22, br. 3, str. 138-142
jezik rada: engleski
vrsta rada: izvorni naučni članak
objavljeno: 02/11/2018
doi: 10.5937/JPEA1803138P
Proizvodnja agenasa biološke kontrole primenom Bacillus sp. u laboratorijskom bioreaktoru
Univerzitet u Novom Sadu, Tehnološki fakultet

e-adresa: paj@tf.uns.ac.rs

Projekat

Unapređenje proizvodnje bioetanola iz proizvoda prerade šećerne repe (MPNTR - 31002)

Sažetak

Prekomerna upotreba pesticida u zaštiti poljoprivrednih kultura i suzbijanju bolesti biljaka dovela je do narušavanja kvaliteta zemljišta, zagađenja životne sredine i razvoja rezistentnosti patogena prema hemijskim proizvodima koji se uobičajeno primenjuju, što dovodi do potrebe za njihovom primenom u sve većim koncentracijama, a takođe postoji i značajna zabrinutost usled nedovoljno ispitanih i potencijalno štetnih uticaja ovih hemikalija na zdravlje ljudi. Biološka kontrola primenom mikrobioloških biopesticida, to jest ćelija mikroorganizama ili njihovih metabolita, predstavlja pogodnu ekološku alternativu hemijskim pesticidima, koja pritom doprinosi očuvanju životne sredine i omogućava manju toksičnost primenjenih sredstava prema netargetnim organizmima usled veće selektivnosti delovanja, bezbedniju primenu i otvara mogućnost korišćenja u okviru organske poljoprivrede. Cilj ovog rada bio je ispitivanje mogućnosti proizvodnje agenasa biokontrole primenom Bacillus sp., izolovanog iz mladog sira, kao proizvodnog mikroorganizma. Sa ciljem obezbeđivanja optimalnih uslova za izvođenje bioprocesa, kultivacija u trajanju od 96 h je vršena u laboratorijskom bioreaktoru, koji omogućava regulaciju temperature (28°C), odgovarajuću brzinu aeracije (1 vvm) i mešanje (brzina mešanja od 250 o/min).Uzorci kultivacione tečnosti su testirani in vitro primenom disk-difuzione metode protiv tri fitopatogena izolata: dva Xanthomonas campestris soja izolovana iz kupusa i soja Xanthomonas campestris pv. vesicatoria izolovanog iz paprike. Maksimalni prečnici zona inhibicije su dobijeni nakon 96 h kultivacije kod svakog izolata, dok su razlike njihovih vrednosti ukazale na različitu osetljivost odabranih izolata prema proizvedenim agensima. Uz prečnike zona inhibicije u opsegu 31-62 mm, Bacillus sp. je pokazao značajan potencijal za dalje ispitivanje i moguću primenu u biološkoj kontroli fitopatogenih Xanthomonas campestris sojeva.

Ključne reči

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