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Journal of Medical Biochemistry
2010, vol. 29, br. 2, str. 73-77
jezik rada: engleski
vrsta rada: izvorni naučni članak
objavljeno: 13/07/2010
doi: 10.2478/v10011-010-0010-2
Mogućnost predviđanja vulnerabilnosti aterosklerotskog plaka pomoću aktivnosti limfocitne kaspaze-3
aKlinički centar Niš, Centar za medicinsku biohemiju
bUniverzitet u Nišu, Medicinski fakultet, Institut za prevenciju, lečenje i rehabilitaciju reumatičkih i kardioloških bolesti 'Niška Banja' - Niška Banja
cUniverzitet u Nišu, Medicinski fakultet, Institut za biohemiju

e-adresa: ristict@bankerinter.net

Sažetak

Smrt ćelija apoptozom ima značajnu ulogu u različitim kardiovaskularnim oboljenjima, posebno u onim koja se razvijaju na temelju ateroskleroze. Cilj ove studije bilo je poređenje aktivnosti kaspaze-3 u različitim oblicima ishemijske bolesti srca i koreliranje njene aktivnosti sa inflamatornim, lipidnim markerima i faktorima rizika. Aktivnost kaspaze-3 određivana je u mononuklearnim ćelijama periferne krvi (MĆPK) kolorimetrijskim komercijalnim ELISA testom. Enzimska aktivnost određivana je kod 30 pacijenata sa stabilnom anginom pektoris (SAP), 27 sa nestabilnom anginom pektoris (NSAP), 39 sa akutnim infarktom miokarda sa elevacijom ST segmenta (STEMI) i 27 zdravih dobrovoljaca. U MĆPK pacijenata sa SAP aktivnost enzima bila je 0,093±0,036 μmol/mg proteina, a kod pacijenata sa STEMI 0,110±0,062 μmol/mg proteina, i obe vrednosti su bile statistički ne značajno više u poređenju sa kontrolnom grupom (0,092±0,022 μmol/mg proteina). U MĆPK pacijenata sa NSAP aktivnost kaspaze-3 (0,122±0,062 μmol/mg proteina) bila je statistički značajno viša (p<0,05) u poređenju sa kontrolnom grupom. U grupi pacijenata sa SAP postoji statistički značaj na pozitivna korelacija kaspaze-3 i triglicerida (p<0,05). Aktivnost kaspaze-3 može biti validan parametar u praćenju aktivnosti aterosklerotičnog plaka i nova meta za terapijske intervencije.

Ključne reči

ishemijska bolest srca; kaspaza-3; inflamatorni markeri; lipidni markeri

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