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2012, vol. 49, br. 2, str. 160-166
Epicoccum nigrum novi patogen semena sirka u Srbiji
aUniverzitet u Beogradu, Poljoprivredni fakultet
bNaučni institut za ratarstvo i povrtarstvo, Novi Sad
cInstitut za zaštitu bilja i životnu sredinu, Beograd

e-adresaaleksandrabulajic@yahoo.com
Projekat:
Agrobiodiverzitet i korišćenje zemljišta u Srbiji: integrisana procena biodiverziteta ključnih grupa artropoda i biljnih patogena (MPNTR - 43001)
Unapređenje proizvodnje kukuruza i sirka u uslovima stresa (MPNTR - 31073)

Ključne reči: Epicoccum; karakterizacija; sekvencioniranje; seme; sirak
Sažetak
U periodu 2009-2011. godine na lokalitetima Bački Petrovac i Čantavir prikupljeno je i analizirano 16 uzoraka zaraženog semena gajenog sirka (Sorghum bicolor (L.) Moench.) sorti Alba, Gold, Prima i Reform na prisustvo fitopatogenih gljiva. U pojedinačnim i mešanim zarazama ustanovljeno je prisustvo vrsta iz rodova Epicoccum, Fusarium, Alternaria, Aspergillus i Penicillium. Iz zaraženog semena izolovane su monosporne kulture i na osnovu morfoloških osobina identifikovane su kao Epicoccum nigrum. Patogenost izolata ove gljive potvrđena je pojavom simptoma na veštački inokulisanim sejancima sirka. Molekularna identifikacija obavljena je primenom PCR i amplifikacije ITS regiona ribozomalne DNK. Sekvence gena odabranih izolata 291-09 (JQ619838) i 315-09 (JQ619839) pokazale su 99-100% nukleotidne identičnosti sa sekvencama 31 izolata E. nigrum deponovanihu GenBank bazi podataka. Dobijeni rezultati predstavljaju prvu detaljnu karakterizaciju E. nigrum u Srbiji. Prisustvo većeg broja vrsta gljiva na semenu sirka zahteva dalja ispitivanja njihovih međusobnih odnosa i značaja.
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O članku

jezik rada: srpski
vrsta rada: izvorni naučni članak
DOI: 10.5937/ratpov49-1793
objavljen u SCIndeksu: 10.08.2012.
metod recenzije: dvostruko anoniman