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2012, vol. 49, br. 2, str. 151-159
Prisustvo i karakterizacija virusa žutog mozaika cukinija u usevu lubenice u Srbiji
aUniverzitet u Beogradu, Poljoprivredni fakultet
bNaučni institut za ratarstvo i povrtarstvo, Novi Sad

e-adresahomemadeent@gmail.com
Projekat:
Agrobiodiverzitet i korišćenje zemljišta u Srbiji: integrisana procena biodiverziteta ključnih grupa artropoda i biljnih patogena (MPNTR - 43001)
Razvoj novih sorti i poboljšanje tehnologija proizvodnje uljanih biljnih vrsta za različite namene (MPNTR - 31025)

Sažetak
Prisustvo virusa žutog mozaika cukinija (Zucchini yellow mosaic virus, ZYMV) na dva od sedam lokaliteta gajenja lubenice u Srbiji tokom 2011. godine utvrđeno je analizom uzoraka lišća simptomatičnih i asimptomatičnih biljaka lubenice primenom DAS-ELISA metode. Na lokalitetu Gornji Tavankut, ZYMV je dokazan u 23,08% testiranih biljaka u pojedinačnim infekcijama, a na lokalitetu Silbaš u 35,29% testiranih biljaka u mešanim infekcijama sa virusom mozaika krastavca i virusom mozaika lubenice. ZYMV je uspešno mehanički prenesen sa prirodno zaraženih biljaka lubenice na Cucurbita pepo 'Ezra F1'. Molekularna detekcija obavljena je RT-PCR metodom umnožavanjem dela gena za nuklearne inkluzije, gena za protein omotača i dela 3' neprepisujućeg regiona, čime je potvrđena identifikacija izolata. Filogenetske analize pokazale su grupisanje izolata poreklom iz lubenice sa drugim izolatima iz Srbije i Centralne Evrope u okviru A-I podgrupe. Analize aminokiselinske sekvence N-terminalnog kraja CP gena, takođe su pokazale pripadnost izolata 550-11 centralnoevropskoj grani.
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O članku

jezik rada: srpski
vrsta rada: izvorni naučni članak
DOI: 10.5937/ratpov49-1773
objavljen u SCIndeksu: 10.08.2012.
metod recenzije: dvostruko anoniman

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