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2011, vol. 48, br. 2, str. 347-352
Molekularna identifikacija izolata Fusarium graminearum, patogena sirka u Srbiji
aUniverzitet u Beogradu, Poljoprivredni fakultet
bNaučni institut za ratarstvo i povrtarstvo, Novi Sad

e-adresaristicdaca@yahoo.com
Projekat:
Unapređenje proizvodnje kukuruza i sirka u uslovima stresa (MPNTR - 31073)
Agrobiodiverzitet i korišćenje zemljišta u Srbiji: integrisana procena biodiverziteta ključnih grupa artropoda i biljnih patogena (MPNTR - 43001)

Ključne reči: Fusarim graminearum; molekularna identifikacija; morfološke osobine; sirak; TEF gen; testovi patogenosti
Sažetak
U periodu 2009-2011. na lokalitetima u Bačkom Petrovcu i Čantaviru prikupljeno je i analizirano 39 uzoraka biljaka gajenog sirka (Sorghum bicolor) sa simptomima truleži prizemnog dela stabla. Iz biljnog tkiva izolovane su monosporne kulture, čija je patogenost potvrđena pojavom simptoma na veštački inokulisanim biljkama sirka, a na osnovu morfoloških makroskopskih i mikroskopskih osobina identifikovan je Fusarium graminearum. Molekularna identifikacija obavljena je primenom lančane reakcije polimeraze (PCR, Polymerase Chain Reaction) uz korišćenje prajmera ef1/ef2 i amplifikaciju kodirajućeg proteinskog gena TEF 1-alfa. Sekvenca TEF gena odabranog izolata 535-10 (JF747146) je pokazala 98% do 99% nukleotidne identičnosti sa sekvencama 63 izolata Gibberella zeae deponovanih u NCBI bazi podataka. Amplifikacijom barkoding dela genoma F. graminearum izolata iz sirka dat je doprinos bržoj i preciznijoj identifikaciji i karakterizaciji vrsta roda Fusarium u Srbiji.
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O članku

jezik rada: srpski
vrsta rada: izvorni naučni članak
DOI: 10.5937/ratpov1102347R
objavljen u SCIndeksu: 16.08.2011.

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