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2013, vol. 141, br. 3-4, str. 187-191
Infekcija grlića materice bakterijom Chlamydia trachomatis kod studentkinja - dijagnostika klasičnim i molekularnim metodama
aKlinički centar Srbije, Klinika za ginekologiju i akušerstvo, Beograd
bZavod za zdravstvenu zaštitu studenata, Beograd
cUniverzitet u Beogradu, Medicinski fakultet, Institut za mikrobiologiju i imunologiju

e-adresabobadi@sezampro.rs
Sažetak
Uvod. Chlamydia trachomatis je najčešći bakterijski uzročnik polno prenosivih infekcija. Mogućnost razvoja teških komplikacija ovih infekcija može se sprečiti blagovremenim otkrivanjem ove bakterije na grliću materice primenom osetljivih i pouzdanih testova, kao što su savremeni molekularni testovi. Cilj rada. Cilj istraživanja je bio da se ispitaju mogućnost i učestalost dijagnostikovanja infekcije grlića materice bakterijom C. trachomatis kod studentkinja primenom klasičnih i molekularnih metoda. Metode rada. Postojanje C. trachomatis u endocervikalnom brisu 69 studentkinja bez simptoma oboljenja dokazano je primenom tri metode: direktne imunofluorescencije (DIF), hibridizacije nukleinske kiseline (hc2) i reakcije lančanog umnožavanja nukleinske kiseline (PCR). Rezultati. Metodom DIF C. trachomatis je otkrivena kod četiri ispitanice (5,80%), metodom hc2 kod takođe četiri (5,80%), a primenom PCR kod šest studentkinja (8,70%). Uporedna analiza primene DIF s metodom PCR pokazala je senzitivnost od 46% i specifičnost od 95%, uporedna analiza primene DIF s metodom hc2 pokazala je senzitivnost od 62% i specifičnost od 97%, dok je uporedna analiza primene hc2 s metodom PCR pokazala senzitivnost od 76% i specifičnost od 100%. Zaključak. Metoda izbora za dijagnostikovanje C. trachomatis na grliću materice kod mladih osoba bez simptoma oboljenja je PCR, savremena molekularna metoda.
Reference
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O članku

jezik rada: srpski
vrsta rada: originalan članak
DOI: 10.2298/SARH1304187T
objavljen u SCIndeksu: 13.11.2013.

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