Metrika članka

  • citati u SCindeksu: 0
  • citati u CrossRef-u:0
  • citati u Google Scholaru:[=>]
  • posete u poslednjih 30 dana:70
  • preuzimanja u poslednjih 30 dana:20
članak: 1 od 1  
Back povratak na rezultate
Journal of Medical Biochemistry
2020, vol. 39, br. 1, str. 100-107
jezik rada: engleski
vrsta rada: izvorni naučni članak
objavljeno: 10/01/2020
doi: 10.2478/jomb-2019-0039
Creative Commons License 4.0
Procena određivanja 25-OH vitamina D na Abbott Architect analizatoru pri različitim nivoima deficita vitamina 25-OH D
aPamukkale University, Faculty of Medicine, Department of Biochemistry, Denizli, Turkey
bPamukkale University, Faculty of Medicine, Department of Biostatistics, Denizli, Turkey

e-adresa: hekimesin@gmail.com

Sažetak

Uvod: Poslednjih godina povećan je zahtev za određivanjem vitamina D u mnogim kliničkim slučajevima. Međutm, imuno određivanje je i dalje kontraverzno. Nekoliko dijagnostičkih proizvođača razvilo je poslednjih godina automatsko imunoodređivanje 25-hidroksivitamina D (25-OH D). Mi smo poredili imunohemijsko određivanje 25-OH vitamina D pomoću Abbott Architect analizatora sa tandem sistemom tečna hromatografija-masena spektrometrija (LS-MS/MS) kako bi procenili imunohemijsko određivanje naročito u deficijentnim grupama. Metode: Analizirano je 80 humanih uzoraka seruma primenom Architect 25-OH D vitamin reagensom (Abbott Diagnostics, Lake Forest, IL, USA) i LC-MS/MS (Zivak Technology, Istanbul, Turkey). Dobijeni rezultati primenom imunoodređivanja bili su u korelaciji sa rezultatima dobijenim sistemom tečna hromatografija-masena spektrometrija (LSMS/MS) korišćenjem Passing-Bablok regresionom analizom, Bland-Altman metodom i analizom korelacionog koeficijenta. Takođe smo procenjivali rezultate četiri nivoa vitamina D kao tešku deficijenciju, deficijenciju, insuficijenciju i suficit. Rezultati: Arhitect je prikazao 9,59% odstupanje od LCMS/MS sa neznatnim značajem. Passing-Bablok regresiona analiza ukazala je na vrednost nagiba od 0,95 i konstantom Korelacioni koeficijent se umereno slagao sa vrednostima 0,918 (95% CI 0,878-0,945). Dve metode su imale dobro slaganje (kappa = 0,738). Najmanje odstupanje je određeno u grupi sa deficijencijom (0,95%), a najveće u grupi sa insuficijencijom (15,15%). Zaključak: Imunodređivanje sa Arhitect 25-OH D vitaminom može da se koristi kao rutinsko merenje mada ima potencijalni nedostatak pri klasifikaciji statusa vitamina D u grupama sa insuficijencijom i deficijencijom. Mada postoje skorašnji pokušaji standardizacije merenja 25-OH D kliničke laboratorije moraju ovo da imaju na umu.

Ključne reči

vitamin; poređenje metoda; deficijencija; insuficijencija; 25-hiodroksivitamin D

Reference

*** (1825) A. V. D. package insert 510k 25-OH D: Decision Summary Assay Only Template. vol. 510, pp. 1-17
*** (2003) Prevention and management of osteoporosis: Report of a WHO scientific group. Geneva, Switzerland: World Health Organization-WHO Scientific Group on the Prevention and Management of Osteoporosis, 2000
Annema, W., Nowak, A., von Eckardstein, A., Saleh, L. (2018) Evaluation of the new restandardized Abbott Architect 25-OH Vitamin D assay in vitamin D-insufficient and vitamin D-supplemented individuals. Journal of Clinical Laboratory Analysis, 32(4): e22328-e22328
Atef, S.H. (2018) Vitamin D assays in clinical laboratory: Past, present and future challenges. Journal of Steroid Biochemistry and Molecular Biology, 175: 136-137
Bess, D. Vitamin D deficiency in adults: Definition, clinical manifestations, and treatment. UpToDate Inc, https://www.uptodate.com/contents/vitamin-d-deficiency-in-adults-definition-clinical-manifestations-and-treatment (Accessed on March 31, 2019)
Çakır, M.Ö., Orçun, A., Yıldız, Z., Sirmali, R. (2017) Evaluation of new Beckman Coulter 25(OH) Vitamin D assay and potential improvement of clinical interpretation. Biochemia Medica, 25: 332-373
Cavalier, E., Carlisi, A., Bekaert, A., Rousselle, O., Chapelle, J., Souberbielle, J. (2012) Analytical evaluation of the new Abbott Architect 25-OH vitamin D assay. Clinical Biochemistry, 45(6): 505-508
Cavalier, E., Lukas, P., Bekaert, A., Carlisi, A., Le, G.C., Delanaye, P., Souberbielle, J. (2017) Analytical and clinical validation of the new Abbot Architect 25(OH)D assay: Fit for purpose?. Clinical Chemistry and Laboratory Medicine (CCLM), 55(3): 378-84
Clarke, N.M., Page, J.E. (2012) Vitamin D deficiency: A paediatric orthopaedic perspective. Current Opinion in Pediatrics, 24(1): 46-55
Damiati, S. (2019) A pilot study to assess Kidney functions and toxic dimethyl-arginines as risk biomarkers in women with low vitamin D levels. Journal of Medical Biochemistry, vol. 38, br. 2, str. 145-152
Dietrich, A., Volmer, L. (2015) Analysis of Vitamin D Metabolic Markers by Mass Spectrometry: Current Techniques, Limitations of the 'Gold Standard' Method, and Anticipated Future Directions. Mass Spectrometry Reviews, 34: 2-23
Dreier, N., Lykkedegn, S., Back, P., et al. (2017) S-25-hydroxyvitamin D and C3-epimers in pregnancy and infancy: An Odense Child Cohort study. Clinical Biochemistry, (2): 1-1
Farrell, C., Martin, S., McWhinney, B., Straub, I., Williams, P., Herrmann, M. (2012) State-of-the-Art Vitamin D Assays: A Comparison of Automated Immunoassays with Liquid Chromatography-Tandem Mass Spectrometry Methods. Clinical Chemistry, 58(3): 531-542
Gallagher, C.J., Sai, A.J. (2010) Vitamin D Insufficiency, Deficiency, and Bone Health. Journal of Clinical Endocrinology & Metabolism, 95(6): 2630-2633
Gören, A.C., Şahin, G., Gümilcineli, İ., Binici, B. (2018) Rapid and reliable 25-OH vitamin D2 and 25-OH vitamin D3 measurements by multitasker LC-MS/MS. Journal of Chemical Metrology, 12(1): 17-25
Grebe, S.K.G., Singh, R.J. (2011) LC-MS/MS in the Clinical Laboratory: Where to from Here?. Clin Biochem Rev, 32(1): 5-31
Hutchinson, K., Healy, M., Crowley, V., Louw, M., Rochev, Y. (2017) Verification of Abbott 25-OH-vitamin D assay on the architect system. Practical Laboratory Medicine, 7: 27-35
Jakobsen, J., Bysted, A., Andersen, R., Bennett, T., Brot, C., Bügel, S., Cashman, K.D., Denk, E., Harrington, M., Teucher, B., Walczyk, T., Ovesen, L. (2009) Vitamin D status assessed by a validated HPLC method: Within and between variation in subjects supplemented with vitamin D3. Scandinavian Journal of Clinical and Laboratory Investigation, 69(2): 190-197
Kiani, A., Abedini, A., Adcock, I.M., Sadat-Mirenayat, M., Taghavi, K., Mortaz, E., Kazempour-Dizaji, M. (2018) Association between vitamin D deficiencies in sarcoidosis with disease activity, course of disease and stages of lung involvements. Journal of Medical Biochemistry, vol. 37, br. 2, str. 103-109
Manuilova, E., Schuetzenmeister, A., Model, F. (2014) mcr: Method Comparison Regression. R package version 1.2.1., https://CRAN.R-project.org/package=mcr
Marshall, J., Nunes, T., Heuer, C., Marshall, J., et al. (2015) RStudio: Integrated Development for R. Boston, MA: RStudio, Inc-RStudio Team, URL http://www.rstudio.com
Ozcan, N., Ucar, F., Arzuhal, A.E., Bulut, E., Ozturk, A., Taslipinar, Y.M., Temel, I., Erden, G. (2016) Evaluation of the analytical performance of Unicel DXI 800 for the Total 25 (OH) Vitamin D measurements. Clinical Biochemistry, 49(6): 486-491
Sahota, O. (2014) Understanding vitamin D deficiency. Age and Ageing, 43(5): 589-591
Sempos, C.T., Vesper, H.W., Phinney, K.W., Thienpont, L.M., Coates, P.M. (2012) Vitamin D status as an international issue: National surveys and the problem of standardization. Scandinavian Journal of Clinical and Laboratory Investigation, 72: Suppl 243: 32-40
Sezgin, G., Ozturk, G., Turkal, R., Caykara, B. (2019) Vitamin D levels of outpatients admitted to a University Hospital in the Marmara region of Turkey over 3 years. Journal of Medical Biochemistry, vol. 38, br. 2, str. 181-187
Shah, I.J., James, R., Barker, J., Petroczi, A., Naughton, D.P. (2011) Misleading measures in Vitamin D analysis: A novel LC-MS/MS assay to account for epimers and isobars. Nutrition Journal, 10(1): 46-46
Speeckaert, M.M., Speeckaert, R., van Geel, N., Delanghe, J.R. (2014) Vitamin D Binding Protein: A Multifunctional Protein of Clinical Importance. u: Makowski Gregory S. [ur.] Advances in Clinical Chemistry, Elsevier, Chapter One, 63: 1-57
Stepman, H.C.M., Vanderroost, A., van Uytfanghe, K., Thienpont, L.M. (2011) Candidate Reference Measurement Procedures for Serum 25-Hydroxyvitamin D3 and 25-Hydroxyvitamin D2 by Using Isotope-Dilution Liquid Chromatography-Tandem Mass Spectrometry. Clinical Chemistry, 57(3): 441-448
Thacher, T.D., Clarke, B.L. (2011) Vitamin D Insufficiency. Mayo Clinic Proceedings, 86(1): 50-60
Topçuoğlu, C., Sezer, S., Yılmaz, F.M., Kösem, A., Ercan, M., Turhan, T. (2018) Evaluation of the analytical performance of the Beckman Coulter Unicel DXI 800 Access Total 25(OH) Vitamin D immunoassay. Laboratoriums Medizin, 42(5): 205-211