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2017, vol. 56, br. 4, str. 75-82
Uticaj terapije metforminom na parametre oksidativnog oštećenja u retini pacova sa poremećenom tolerancijom glukoze
aUniverzitet u Nišu, Medicinski fakultet, Katedra Biohemija
bUniverzitet u Nišu, Medicinski fakultet, Katedra Histologija sa embriologijom
cUniverzitet u Nišu, Medicinski fakultet, Katedra Patofiziologija
dUniverzitet u Nišu, Medicinski fakultet

e-adresabrankadjordjevic83@gmail.com
Projekat:
Monitoring elektromagnetnih zračenja mobilnih telekomunikacionih sistema u životnoj sredini, analiza molekularnih mehanizama i biomarkera oštećenja kod hronične izloženosti sa razvojem modela za procenu rizika i metoda za zaštitu (MPNTR - 43012)

Ključne reči: dijabetesna retinopatija; oksidativni stres; poremećena tolerancija glukoze
Sažetak
Poremećena tolerancija glukoze je poremećaj koji se karakteriše povišenim vrednostima glikemije, ali ipak nedovoljno visokim da bi se postavila dijagnoza šećerne bolesti. Dijabetesna retinopatija je jedna od komlikacija šećerne bolesti za čiju je patogenezu značajan oksidativni stres. Metformin je trenutno lek prvog izbora u terapiji dijabetesa melitusa tip 2. Cilj rada bio je da na animalnom modelu utvrdimo da li postoji oksidativno oštećenje retine u poremećenoj toleranciji glukoze (analizom tiobarbiturat-reagujućih supstanci - TBARS - i uznapredovalih produkata oksidacije proteina - AOPP) i da li to oštećenje može biti ublaženo primenom metformina. Eksperiment je izveden na 10 nedelja starim Wistar pacovima, koji su nasumično podeljeni u četiri grupe. Poremećena tolerancija glukoze izazvana je pomoću intraperitonealne injekcije rastvora streptozotocina (STZ), koja je data 15 minuta nakon intraperitonealne injekcije nikotinamida. Nakon četiri nedelje, uvedena je terapija metforminom (100 mg/kg, per os). Nakon dve nedelje, životinje su žrtvovane u dubokoj anesteziji. Koncentracije TBARS i AOPP u homogenatu retine bile su značajno više kod životinja sa poremećenom tolerancijom glukoze u poređenju sa kontrolom (za TBARS: 4,09 ± 0,39 vs. 2,98 ± 0,26; p < 0,001 i za AOPP: 34,49 ± 3,21 vs. 26,26 ± 3,16; p <0,001). Utvrđeno je i postojanje jake pozitivne korelacije između vrednosti glikemije i nivoa TBARS (r = 0,757, p < 0,01 (grafikon 4) i nivoa AOPP (r = 0,683, p < 0,01) u retini. Metformin nije pokazao značajne efekte na koncentracije ispitivanih parametara. Vrednosti ispitivanih parametara oksidativnog oštećenja kod životinja sa poremećenom tolerancijom glukoze ukazuju na postojanje intenzivnijeg oksidativnog stresa u retini, što predstavlja prvi korak u nastanku dijabetesne retinopatije. Terapija metforminom u dozi od 100 mg/kg nije pokazala značajne korisne efekte na proces lipidne peroksidacije i oksidaciju proteina u tkivu retine kod životinja sa poremećenom tolerancijom glukoze.
Reference
Alhaider, A.A., Korashy, H.M., Sayed-Ahmed, M.M., Mobark, M., Kfoury, H., Mansour, M.A. (2011) Metformin attenuates streptozotocin-induced diabetic nephropathy in rats through modulation of oxidative stress genes expression. Chemico-Biological Interactions, 192(3): 233-242
Al-Shabrawey, M., Smith, S. (2010) Prediction of diabetic retinopathy: role of oxidative stress and relevance of apoptotic biomarkers. EPMA Journal, 1(1): 56-72
Andreeva, L.I., Kozhemiakin, L.A., Kishkun, A.A. (1988) Modification of the method of determining lipid peroxidation in a test using thiobarbituric acid. Lab Delo, (11): 41-3
Armstrong, D., Abdella, N., Salman, A., Miller, N., Rahman, E.A., Bojancyzk, M. (1992) Relationship of lipid peroxides to diabetic complicationsComparison with conventional laboratory tests. Journal of Diabetes and its Complications, 6(2): 116-122
Bertram, M.Y., Vos, T. (2010) Quantifying the duration of pre-diabetes. Australian and New Zealand Journal of Public Health, 34(3): 311-314
Bhattacharya, S. (2015) Reactive oxygen species and cellular defense system. u: Rani V., Yadav U.C.S. [ur.] Free Radicals Hum. Heal. Dis, New Delhi: Springer India
Cade, W.T. (2008) Diabetes-Related Microvascular and Macrovascular Diseases in the Physical Therapy Setting. Physical Therapy, 88(11): 1322-1335
Çakatay, U. (2005) Protein oxidation parameters in type 2 diabetic patients with good and poor glycaemic control. Diabetes & Metabolism, 31(6): 551-557
Ceriello, A., Esposito, K., Piconi, L., Ihnat, M. A., Thorpe, J. E., Testa, R., Boemi, M., Giugliano, D. (2008) Oscillating Glucose Is More Deleterious to Endothelial Function and Oxidative Stress Than Mean Glucose in Normal and Type 2 Diabetic Patients. Diabetes, 57(5): 1349-1354
Chakraborty, A., Chowdhury, S., Bhattacharyya, M. (2011) Effect of metformin on oxidative stress, nitrosative stress and inflammatory biomarkers in type 2 diabetes patients. Diabetes Research and Clinical Practice, 93(1): 56-62
Diabetes Prevention Program Research Group (2007) The prevalence of retinopathy in impaired glucose tolerance and recent-onset diabetes in the Diabetes Prevention Program. Diabetic Medicine, 24(2): 137-144
Esteghamati, A., Eskandari, D., Mirmiranpour, H., Noshad, S., Mousavizadeh, M., Hedayati, M., Nakhjavani, M. (2013) Effects of metformin on markers of oxidative stress and antioxidant reserve in patients with newly diagnosed type 2 diabetes: A randomized clinical trial. Clinical Nutrition, 32(2): 179-185
Giacco, F., Brownlee, M. (2010) Oxidative Stress and Diabetic Complications. Circulation Research, 107(9): 1058-1070
Kalousová, M., Skrha, J., Zima, T. (2002) Advanced glycation end-products and advanced oxidation protein products in patients with diabetes mellitus. Physiol. Res, 51, 597-604
Kowluru, R.A., Chan, P. (2007) Oxidative Stress and Diabetic Retinopathy. Experimental Diabetes Research, 2007: 1-12
Kumari, S., Panda, S., Mangaraj, M., Mandal, M. K., Mahapatra, P. C. (2008) Plasma MDA and antioxidant vitamins in diabetic retinopathy. Indian Journal of Clinical Biochemistry, 23(2): 158-162
Lowry, O.H., Farral, R.N.J., Randall, R.J. (1951) Protein measurement with the Folin phenol reagent. J. Biol. Chem., 193: 265-75
Mandal, N., Lewis, G.P., Fisher, S.K., Heegaard, S., Prause, J.U., la Cour, M., i dr. (2011) Protein changes in the retina following experimental retinal detachment in rabbits. Mol. Vis., 17: 2634-48
Martin-Gallan, P., Carrascosa, A., Gussinye, M., Dominguez, C. (2003) Biomarkers of diabetes-associated oxidative stress and antioxidant status in young diabetic patients with or without subclinical complications. Free Radic Biol Med, 34(12): 1563-74
Masiello, P., Broca, C., Gross, R., Roye, M., Manteghetti, M., Hillaire-Buys, D., Novelli, M., Ribes, G. (1998) Experimental NIDDM: development of a new model in adult rats administered streptozotocin and nicotinamide. Diabetes, 47(2): 224-229
Monnier, L., Mas, E., Ginet, C., Michel, F., Villon, L., Cristol, J., Colette, C. (2006) Activation of Oxidative Stress by Acute Glucose Fluctuations Compared With Sustained Chronic Hyperglycemia in Patients With Type 2 Diabetes. JAMA, 295(14): 1681
Pari, L., Ashokkumar, N. (2005) Effect of N-benzoyl-Dphenylalanine, a new potential oral antidiabetic agent, in neonatal streptozotocin-induced diabetes in rats. Pharmacol. Rep., 57: 498-503
Salido, E.M., de Zavalía, N., Schreier, L., de Laurentiis, A., Rettori, V., Chianelli, M., Keller, S.M.I., Arias, P., Rosenstein, R.E. (2012) Retinal changes in an experimental model of early type 2 diabetes in rats characterized by non-fasting hyperglycemia. Experimental Neurology, 236(1): 151-160
Shi, X., Liao, S., Mi, H., Guo, C., Qi, D., Li, F., Zhang, C., Yang, Z. (2012) Hesperidin Prevents Retinal and Plasma Abnormalities in Streptozotocin-Induced Diabetic Rats. Molecules, 17(12): 12868-12881
Tarr, J.M., Kaul, K., Chopra, M., Kohner, E.M., Chibber, R. (2013) Pathophysiology of Diabetic Retinopathy. ISRN Ophthalmology, 2013: 1-13
Tzekov, R., Stein, L., Kaushal, S. (2011) Protein Misfolding and Retinal Degeneration. Cold Spring Harbor Perspectives in Biology, 3(11): a007492-a007492
Witko-Sarsat, V., Friedlander, M., Capeillere-Blandin, C., Nguyen-Khoa, T., Nguyen, A.T., Zingraff, J., Jungers, P., Descamps-Latscha, B. (1996) Advanced oxidation protein products as a novel marker of oxidative stress in uremia. Kidney Int, 49(5): 1304-13
World Health Organization (2014) Global status report on noncommunicable diseases 2014. Geneva
World Health Organisation (1999) Definition, diagnosis and classification of diabetes mellitus and its complications, part 1: Diagnosis and classification of diabetes mellitus. Geneva
Yao, X., Ye, S., Xiao, C., Gu, J., Yang, D., Wang, S. (2012) Metformin alleviates high glucose-mediated oxidative stress in rat glomerular mesangial cells by modulation of p38 mitogen-activated protein kinase expression in vitro. Molecular Medicine Reports, 12(1): 520-526
Yülek, F., Or, M., Özoğul, C., Isik, A.C., Ari, N., Stefek, M., Bauer, V., Karasu, C. (2007) Effects of Stobadine and Vitamin E in Diabetes-Induced Retinal Abnormalities: Involvement of Oxidative Stress. Archives of Medical Research, 38(5): 503-511
 

O članku

jezik rada: engleski
vrsta rada: izvorni naučni članak
DOI: 10.5633/amm.2017.0412
objavljen u SCIndeksu: 15.03.2018.
Creative Commons License 4.0

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