Metrika članka

  • citati u SCindeksu: 0
  • citati u CrossRef-u:0
  • citati u Google Scholaru:[=>]
  • posete u poslednjih 30 dana:6
  • preuzimanja u poslednjih 30 dana:6
članak: 5 od 8  
Back povratak na rezultate
Medicinski podmladak
2015, vol. 66, br. 2, str. 55-60
jezik rada: engleski
vrsta rada: izvorni naučni članak
doi:10.5937/medpodm1502055B


Bacterial contamination of stethoscopes in university hospitals: Multicenter study
(naslov ne postoji na srpskom)
aUniverzitet u Beogradu, Medicinski fakultet
bFaculty of Medicine, Benha University, Egypt
cHospital Clinic, University of Barcelona, Spain
dUniverzitet u Beogradu, Medicinski fakultet, Institut za mikrobiologiju i imunologiju

e-adresa: stefan92boskovic@yahoo.com

Sažetak

Uvod: Membrana stetoskopa predstavlja pogodnu sredinu za preživljavanje bakterija i kontaminira se prilikom pregleda pacijenata. Ukoliko se ne dezinfikuje, može predstavljati izvor infekcije i doprineti razvoju bolničkih infekcija. Cilj: Cilj ovog rada je ustanoviti učestalost kontaminacije membrana stetoskopa, identifikovati prisutne mikroorganizme, ispitati njihovu osetljivost na antimikrobne lekove i utvrdi da li postoji razlika u kontaminaciji membrana stetoskopa u zavisnosti od redovnosti čišćenja u univerzitetskim klinikama u Beogradu, Benhi i Barseloni. Materijal i metode: Brisevi membrana stetoskopa su uzeti od 147 lekara zaposlenih u jednoj od tri univerzitetske klinike u Beogradu, Benhi ili Barseloni. Lekari su anketirani o navikama održavanja stetoskopa. Materijal je zasejan i inkubiran u dekstrozni bujon i na krvni agar. Nakon porasta kolonija identifikovane su na osnovu kulturelnih, mikroskopskih i biohemijskih osobina i upotrebom MALDI-TOF. Osetljivost na anti-mikrobne lekove ispitana je disk difuzionim metodom. Rezultati: Samo 12 lekara svoje stetoskope dezinfikuje nakon pregleda svakog pacijenta. 122 uzorka su bila kontaminirana, 25 sterilna (1 iz Beograda i 24 iz Benhe). Ne postoji statistički značajna razlika u stepenu kontaminacije stetoskopa i redovnosti čišćenja (p > 0,05). Stetoskopi u Benhi su statistički značajno manje kontaminirani (p < 0,05). Izolovane bakterije pripadaju rodovima Staphylococcus (125 sojeva), Streptococcus (7 sojeva), Bacillus (5 sojeva), Corynebacterium (13 sojeva), Acinetobacter (2 soja), Proteus (2 soja), Enterococcus (1 soj) i Pseudomonas (1 soj). Rezistencija na meticilin koagulaza negativnih stafilokoka iznosi 37,1% u Beogradu, a u Benhi 95,2%. Izolovana su 4 soja Staphylococcus aureus, 3 su bila rezistentna na meticilin. Zaključak: Dezinfekciju membrane stetoskopa bi trebalo sprovoditi posle pregleda svakog pacijenta i neophodna je edukacija lekara o njenom značaju.

Ključne reči

Reference

Centers for Disease Control and Prevention (2008) Guideline for disinfection and sterilization in healthcare facilities. Atlanta (USA): CDC, http://www.cdc.gov/hicpac/Disinfection_Sterilization/acknowledg.html
Croxatto, A., Prod'hom, G., Greub, G. (2012) Applications of MALDI-TOF mass spectrometry in clinical diagnostic microbiology. FEMS Microbiology Reviews, 36(2): 380-407
European Centre for Disease Prevention and Control (2014) Annual epidemiological report Antimicrobial resistance and healthcare-associated infections. Stockholm, Available online from: http://ecdc.europa.eu/en/publications/Publications/antimicrobial-resistance-annual-epidemiological-report.pdf
European Committee on Antimicrobial Susceptibility Testing (2015) Breakpoint tables for interpretation of MICs and zone diameters. Version 5.0, http://www.eucast.org
Kim, S.S., Yu, S., Kim, J., Ryu, S.J. (2013) Comparison of disinfective power according to application order of 70% isopropyl alcohol and 10% povidone-iodine. Korean Journal of Anesthesiology, 65(6): 519
Leontsini, F., Papapetropoulos, A., Vantarakis, A. (2013) Stethoscopes as vectors of multi-resistant coagulase negative staphylococci in a tertiary hospital. International Journal of Medical Science and Public Health, 2(2): 324
Longtin, Y., Schneider, A., Tschopp, C., Renzi, G., Gayet-Ageron, A., Schrenzel, J., Pittet, D. (2014) Contamination of Stethoscopes and Physicians' Hands After a Physical Examination. Mayo Clinic Proceedings, 89(3): 291-299
Magiorakos, A.-P., Srinivasan, A., Carey, R.B., Carmeli, Y., Falagas, M.E., Giske, C.G., Harbarth, S., Hindler, J.F., Kahlmeter, G., Olsson-Liljequist, B., Paterson, D.L., Rice, L.B., Stelling, J., Struelens, M.J., Vatopoulos, A., Weber, J.T., Monnet, D. (2012) Multidrug-resistant, extensively drug-resistant and pandrug-resistant bacteria: an international expert proposal for interim standard definitions for acquired resistance. Clinical Microbiology and Infection, 18(3): 268-281
Mahon, C.R., Lehman, D.C., Manuselis, G. (2012) Textbook of diagnostic microbiology. Maryland Heights, MO: Saunders
Maluf, M.E.Z., Maldonado, A.F., Bercial, M.E., Pedroso, S.A. (2002) Stethoscope: a friend or an enemy?. Sao Paulo Medical Journal, 120(1): 13-15
Neely, A.N., Maley, M.P. (2000) Survival of enterococci and staphylococci on hospital fabrics and plastic. J Clin. Microbiol, 38: 724-726
Parmar, R.C., Valvi, C.C., Sira, P., Kamat, J.R. (2004) A prospective, randomised, double-blind study of comparative efficacy of immediate versus daily cleaning of stethoscope using 66% ethyl alcohol. Indian J Med Sci., 58: 423-430
Shiferaw, T., Beyene, G., Kassa, T., Sewunet, T. (2013) Bacterial contamination, bacterial profile and antimicrobial susceptibility pattern of isolates from stethoscopes at Jimma University Specialized Hospital. Annals of Clinical Microbiology and Antimicrobials, 12(1): 39
Shobha, K., Rao, P., Thomas, J. (2005) Survey of Staphylococcus isolates among hospital personnel, environment and their antibiogram with special emphasis on methicillin resistance. Indian Journal of Medical Microbiology, 23(3): 186
Wilkins, R.L., Restrepo, R.D., Bourne, K.C., Daher, N. (2007) Contamination Level of Stethoscopes Used By Physicians and Physician Assistants. Journal of Physician Assistant Education, 18(2): 41-43
Youngster, I., Berkovitch, M., Heyman, E., Lazarovitch, Z., Goldman, M. (2008) The stethoscope as a vector of infectious diseases in the paediatric division. Acta Paediatrica, 97(9): 1253-1255