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Journal of Medical Biochemistry
2008, vol. 27, br. 3, str. 376-382
jezik rada: engleski
vrsta rada: pregledni članak
doi:10.2478/v10011-008-0019-y
Značaj određivanja urinarnih markera u praćenju dijabetesne nefropatije
aInstitute of Biochemistry, Medical Faculty, Niš + Clinic of Nephrology, Clinical Centre, Niš
bClinic of Nephrology, Clinical Centre, Niš
cInstitute of Biochemistry, Medical Faculty, Niš + Centre for Medical Biochemistry, Clinical Centre, Niš
dCentre for Medical Biochemistry, Clinical Centre, Niš
eInstitute of Biochemistry, Medical Faculty, Niš

e-adresa: milana@bankerinter.net

Sažetak

Oksidativni stres može se smatrati jedinstvenim faktorom koji povezuje dijabetes melitus (DM) i njegove komplikacije, uključujući nefropatiju (DN). Cilj ovog istraživanja bio je da se u urinu pacijenata sa DN odrede parametri oksidativnog oštećenja lipida i proteina kao i aktivnost ektoenzima. Istraživanjem je obuhvaćeno 40 pacijenata: 10 pacijenata sa dijabetes melitusom tipa 2 i mikroalbuminurijom (DMT2-MIA), 10 dijabetičnih pacijenata tipa 2 sa makroalbuminurijom (DMT2-MAA), 10 pacijenata sa dijabetom tipa 1 i mikroalbuminurijom (DMT1-MIA) i 10 zdravih osoba (kontrola). U urinu je određivana koncentracija TBA reaktivnih supstanci (TBARS), reaktivne karbonilne grupe (RCG) i aktivnost ektoenzima N-acetil-b-d-glukozaminidaze (NAG), plazma ćelijski diferencirajući antigen (PC-1), aminopeptidaza N (APN) i dipeptidil peptidaza IV (DPP IV). Veća koncentracija TBARS u urinu nađena je u DMT2-MIA i DMT1- MIA grupi, u odnosu na kontrolu (p<0,001 i p<0,05). Koncentracija RCD u urinu pokazuje slične vrednosti sa statistički značajnim povećanjem u DMT2-MAA i DMT1-MIA grupi u odnosu na DMT2-MIA (p<0,001) i kontrolnu grupu (p<0,001). Aktivnost NAG, APN i DPPIV značajno je veća u urinu pacijenata sa DMT2-MAA u odnosu na kontrolu (p<0,01). Aktivnost PC-1 pokazuje lagani, ali ne i signifikantan porast u toj grupi pacijenata. Zaključujemo da praćenje markera oksidativnog stresa i aktivnost brush border ektoenzima u urinu mogu biti korisni, neinvazivni i lako primenljivi testovi u praćenju DN.

Ključne reči

oksidativni stres; ektoenzimi; urin; dijabetes nanefropatija

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