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Vojnosanitetski pregled
2017, vol. 74, br. 8, str. 715-721
jezik rada: engleski
vrsta rada: izvorni naučni članak
doi:10.2298/VSP150917260T

Creative Commons License 4.0
Produkcija karbapenemaza kod bolničkih multirezistentnih sojeva Klebsiella pneumoniae i Escherichia coli u Srbiji
aInstitute for Pulmonary Diseases of Vojvodina, Sremska Kamenica + University of Novi Sad, Faculty of Medicine, Novi Sad
bUniversity of Novi Sad, Faculty of Medicine, Novi Sad + Institute for Public Health of Vojvodina, Novi Sad
cInstitute for Public Health of Vojvodina, Novi Sad

e-adresa: anika.trudic@mf.uns.ac.rs

Sažetak

Uvod/Cilj. Rezistencija na karbapeneme među medicinski značajnim enterobakterijama kao što su Klebsiella pneumoniae i Escherichia coli u porastu je širom sveta. Zabrinjavajuća je činjenica da su terapijske mogućnosti kod infekcija uzrokovanih multirezistentnim sojevima ograničene ili ih nema. Do sada nije rađena sveobuhvatnija studija o produkciji karbapenemaza kod enterobakterija u Srbiji. Cilj istraživanja bio je utvrđivanje produkcije karbapenemaza kod bolničkih multirezistentnih sojeva K. pneumoniae i E. coli u Srbiji. Metode. Izolati K. pneumoniae i E. coli rezistentni na najmanje jedan karbapenem (imipenem, meropenem, ertapenem) prikupljani su od novembra 2013. do maja 2014. Bakterijski sojevi izolovani su iz klinički značajnih uzoraka od bolesnika lečenih u 14 bolnica u Srbiji. Rezistencija na karbapeneme potvrđena je fenotipskim testom i reakcijom lančane polimerizacije u Nacionalnoj referentnoj laboratoriji za registrovanje i praćenje rezistencije bakterijskih sojeva na antimikrobna sredstva u Novom Sadu. Rezultati. Od ukupno 129 prikupljenih sojeva, bio je 121 (93,8%) izolat K. pneumoniae i 8 (6,2%) izolata E. coli. Iz urina je izolovano 70 (54,3%) sojeva, iz krvi 26 (20,2%), iz sekreta rana 19 (14,7%) i 14 (10,9%) iz sekreta donjeg respiratornog trakta. Geni koji kodiraju karbapenemaze su nađeni kod 58 (45%) izolata. Gen bla New Delhi metallobeta- lactamases (blaNDM), dokazan je kod 33 (27,3%) izolata K. pneumoniae, bla oxacillinases-48 (blaOXA-48) kod 10 (8,3%), bla K. pneumonia carbapenemase (blaKPC) kod 1 (0,8%), a kod 7 (5,4%) izolata su istovremeno nađeni geni blaOXA-48 i blaNDM. Kod 7 izolata E. coli su detektovani blaNDM geni. Zaključak. Najčešći tip karbapenemaza u Srbiji kod multirezistentnih izolata K. pneumoniae i E. coli je NDM. Istovremena produkcija OXA-48 i NDM detektovana je kod izolata K. pneumoniae. Prema našem saznanju, prvi put je nađena produkcija KPC u Srbiji.

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Reference

Barguigua, A., El, otmani F., Lakbakbi, el yaagoubi F., Talmi, M., Zerouali, K., Timinouni, M. (2012) First report of a Klebsiella pneumoniae strain coproducing NDM-1, VIM-1 and OXA-48 carbapenemases isolated in Morocco. APMIS, 121(7): 675-677
Ben, N.A., Decre, D., Compain, F., Genel, N., Barguellil, F., Arlet, G. (2013) Emergence of NDM-1 in Association with OXA-48 in Klebsiella pneumoniae from Tunisia. Antimicrobial Agents and Chemotherapy, 57(8): 4089-4090
Bogaerts, P., Bouchahrouf, W., de Castro, R. R., Deplano, A., Berhin, C., Pierard, D., Denis, O., Glupczynski, Y. (2011) Emergence of NDM-1-Producing Enterobacteriaceae in Belgium. Antimicrobial Agents and Chemotherapy, 55(6): 3036-3038
Carrer, A., Poirel, L., Eraksoy, H., Cagatay, A. A., Badur, S., Nordmann, P. (2008) Spread of OXA-48-Positive Carbapenem-Resistant Klebsiella pneumoniae Isolates in Istanbul, Turkey. Antimicrobial Agents and Chemotherapy, 52(8): 2950-2954
Clinical and Laboratory Standards Institute CLSI (2013) Performance standards for antimicrobial susceptibility testing: Twenty-third informational supplement M100-S23. Wayne, PA
Cohen, S.J., Leverstein-Van, H.M.A. (2010) Guideline for phenotypic screening and confirmation of carbapenemases in Enterobacteriaceae. International Journal of Antimicrobial Agents, 36(3): 205-210
Ellington, M.J., Kistler, J., Livermore, D.M., Woodford, N. (2007) Multiplex PCR for rapid detection of genes encoding acquired metallo-beta-lactamases. Journal of antimicrobial chemotherapy, 59(2): 321-2
Giakkoupi, P., Papagiannitsis, C. C., Miriagou, V., Pappa, O., Polemis, M., Tryfinopoulou, K., Tzouvelekis, L. S., Vatopoulos, A. C. (2011) An update of the evolving epidemic of blaKPC-2-carrying Klebsiella pneumoniae in Greece (2009-10). Journal of Antimicrobial Chemotherapy, 66(7): 1510-1513
Giske, C.G., Gezelius, L., Samuelsen, Ø., Warner, M., Sundsfjord, A., Woodford, N. (2011) A sensitive and specific phenotypic assay for detection of metallo-β-lactamases and KPC in Klebsiella pneumoniae with the use of meropenem disks supplemented with aminophenylboronic acid, dipicolinic acid and cloxacillin. Clinical Microbiology and Infection, 17(4): 552-556
Glasner, C., Albiger, B., Buist, G., Tambić, A.A., Cantón, R., Carmeli, Y., Friedrich, A.W., Giske, C.G., Glupczynski, Y., Gniadkowski, M., Livermore, D.M., Nordmann, P., Poirel, L., Rossolini, G.M., Seifert, H., Vatopoulos, A., Walsh, T., Woodford, N., Donker, T. (2013) Carbapenemase-producing Enterobacteriaceae in Europe: a survey among national experts from 39 countries, February 2013. Eurosurveillance, 18(28):
Grundmann, H., Livermore, D.M., Giske, C.G., Canton, R., Rossolini, G.M., Campos, J., et al. (2010) Carbapenem-non-susceptible Enterobacteriaceae in Europe: Conclusions from a meeting of national experts. Euro Surveill, 15(46): pii: 19711
Hawkey, P.M. (2015) Multidrug-resistant Gram-negative bacteria: a product of globalization. Journal of Hospital Infection, 89(4): 241-247
Jánvári, L., Damjanova, I., Lázár, A., Rácz, K., Kocsis, B., Urbán, E., Tóth, Á. (2014) Emergence of OXA-162-producing Klebsiella pneumoniae in Hungary. Scandinavian Journal of Infectious Diseases, 46(4): 320-324
Jovcic, B., Lepsanovic, Z., Suljagic, V., Rackov, G., Begovic, J., Topisirovic, L., Kojic, M. (2011) Emergence of NDM-1 Metallo- -Lactamase in Pseudomonas aeruginosa Clinical Isolates from Serbia. Antimicrobial Agents and Chemotherapy, 55(8): 3929-3931
Kilic, A., Baysallar, M. (2015) The First Klebsiella pneumoniae Isolate Co-Producing OXA-48 and NDM-1 in Turkey. Annals of Laboratory Medicine, 35(3): 382
Magiorakos, A.-P., Srinivasan, A., Carey, R.B., Carmeli, Y., Falagas, M.E., Giske, C.G., Harbarth, S., Hindler, J.F., Kahlmeter, G., Olsson-Liljequist, B., Paterson, D.L., Rice, L.B., Stelling, J., Struelens, M.J., Vatopoulos, A., Weber, J.T., Monnet, D. (2012) Multidrug-resistant, extensively drug-resistant and pandrug-resistant bacteria: an international expert proposal for interim standard definitions for acquired resistance. Clinical Microbiology and Infection, 18(3): 268-281
Manchanda, V., Gupta, S., Chopra, R., Verma, N., Kaur, I., Rai, S., Rautela, R., Rawat, D., Singh, N., Bhalla, P. (2011) Development of TaqMan real-time polymerase chain reaction for the detection of the newly emerging form of carbapenem resistance gene in clinical isolates of Escherichia coli, Klebsiella pneumoniae, and Acinetobacter baumannii. Indian Journal of Medical Microbiology, 29(3): 249
Marchaim, D., Navon-Venezia, S., Schwaber, M. J., Carmeli, Y. (2008) Isolation of Imipenem-Resistant Enterobacter Species: Emergence of KPC-2 Carbapenemase, Molecular Characterization, Epidemiology, and Outcomes. Antimicrobial Agents and Chemotherapy, 52(4): 1413-1418
Markovska, R., Schneider, I., Stoeva, T., Bojkova, K., Boyanova, L., Bauernfeind, A., Mitov, I. (2013) First identification of KPC-2 and VIM-1 producing Klebsiella pneumoniae in Bulgaria. Diagnostic Microbiology and Infectious Disease, 77(3): 252-253
Miriagou, V., Cornaglia, G., Edelstein, M., Galani, I., Giske, C.G., Gniadkowski, M., Malamou-Lada, E., Martinez-Martinez, L., Navarro, F., Nordmann, P., Peixe, L., Pournaras, S., Rossolini, G.M., Tsakris, A., Vatopoulos, A., Cantón, R. (2010) Acquired carbapenemases in Gram-negative bacterial pathogens: detection and surveillance issues. Clinical Microbiology and Infection, 16(2): 112-122
Mirovic, V., Tomanovic, B., Lepsanovic, Z., Jovcic, B., Kojic, M. (2012) Isolation of Klebsiella pneumoniae Producing NDM-1 Metallo- -Lactamase from the Urine of an Outpatient Baby Boy Receiving Antibiotic Prophylaxis. Antimicrobial Agents and Chemotherapy, 56(11): 6062-6063
Mirović, V., Carević, B., Stepanović, S., Lepšanović, Z. (2011) An outbreak of infection due to metallo-beta-lactamase-producing Proteus mirabilis in the surgical intensive care unit. Scr Med, 42(2): 75−9
Nordmann, P., Poirel, L. (2013) Strategies for identification of carbapenemase-producing Enterobacteriaceae. Journal of Antimicrobial Chemotherapy, 68(3): 487-489
Nordmann, P., Dortet, L., Poirel, L. (2012) Carbapenem resistance in Enterobacteriaceae: here is the storm!. Trends in Molecular Medicine, 18(5): 263-272
Nordmann, P., Naas, T., Poirel, L. (2011) Global Spread of Carbapenemase-producing Enterobacteriaceae. Emerging Infectious Diseases, 17(10): 1791-1798
Nordmann, P., Poirel, L., Dortet, L. (2012) Rapid Detection of Carbapenemase-producing Enterobacteriaceae. Emerging Infectious Diseases, 18(9): 1503-1507
Papp-Wallace, K. M., Endimiani, A., Taracila, M. A., Bonomo, R. A. (2011) Carbapenems: Past, Present, and Future. Antimicrobial Agents and Chemotherapy, 55(11): 4943-4960
Pirš, M., Andlovic, A., Cerar, T., Žohar-Čretnik, T., Kobola, L., Kolman, J., et al. (2011) A case of OXA-48 carbapenemase-producing Klebsiella pneumoniae in a patient transferred to Slovenia from Libya, November 2011. Euro Surveill, 16(50): 20042
Poirel, L., Savov, E., Nazli, A., Trifonova, A., Todorova, I., Gergova, I., Nordmann, P. (2014) Outbreak Caused by NDM-1- and RmtB-Producing Escherichia coli in Bulgaria. Antimicrobial Agents and Chemotherapy, 58(4): 2472-2474
Poirel, L., Potron, A., Nordmann, P. (2012) OXA-48-like carbapenemases: the phantom menace. Journal of Antimicrobial Chemotherapy, 67(7): 1597-1606
Seiffert, S.N., Marschall, J., Perreten, V., Carattoli, A., Furrer, H., Endimiani, A. (2014) Emergence of Klebsiella pneumoniae co-producing NDM-1, OXA-48, CTX-M-15, CMY-16, QnrA and ArmA in Switzerland. International Journal of Antimicrobial Agents, 44(3): 260-262
Székely, E., Damjanova, I., Jánvári, L., Vas, K.E., Molnár, S., Bilca, D.V., Lőrinczi, L.K., Tóth, Á. (2013) First description of blaNDM-1, blaOXA-48, blaOXA-181 producing Enterobacteriaceae strains in Romania. International Journal of Medical Microbiology, 303(8): 697-700
Tängdén, T., Giske, C. G. (2015) Global dissemination of extensively drug-resistant carbapenemase-producing Enterobacteriaceae: clinical perspectives on detection, treatment and infection control. Journal of Internal Medicine, 277(5): 501-512
Tóth, A., Damjanova, I., Puskás, E., Jánvári, L., Farkas, M., Dobák, A., et al. (2014) Emergence of a colistin-resistant KPC-2-producing Klebsiella pneumoniae ST258 clone in Hungary. Clin Microbiol Infect, 20(1): 27-9
Tzouvelekis, L. S., Markogiannakis, A., Psichogiou, M., Tassios, P. T., Daikos, G. L. (2012) Carbapenemases in Klebsiella pneumoniae and Other Enterobacteriaceae: an Evolving Crisis of Global Dimensions. Clinical Microbiology Reviews, 25(4): 682-707
Yong, D., Toleman, M. A., Giske, C. G., Cho, H. S., Sundman, K., Lee, K., Walsh, T. R. (2009) Characterization of a New Metallo- -Lactamase Gene, blaNDM-1, and a Novel Erythromycin Esterase Gene Carried on a Unique Genetic Structure in Klebsiella pneumoniae Sequence Type 14 from India. Antimicrobial Agents and Chemotherapy, 53(12): 5046-5054
Zujić, A.V., Bedenić, B., Kocsis, E., Mazzariol, A., Sardelić, S., Barišić, M., Plečko, V., Bošnjak, Z., Mijač, M., Jajić, I., Vranić-Ladavac, M., Cornaglia, G. (2014) Diversity of carbapenemases in clinical isolates of Enterobacteriaceae in Croatia-the results of a multicentre study. Clinical Microbiology and Infection, 20(11): O894-O903