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Srpski arhiv za celokupno lekarstvo
2018, vol. 146, br. 7-8, str. 417-421
Molekularna dijagnoza bakterijske vaginoze - zastupljenost Gardnerella vaginalis i Atopobium vaginae kod trudnica
Matić Snežanaa, Nenadić Daneb, Čukić Jelenac ORCID, Mijailović Željkod, Manojlović Nevenae, Sazdanović Predragf, Pavlović Milošg, Baskić Dejanh ORCID, Živanović Aleksandarf ORCID
aKlinički centar Kragujevac
bVojnomedicinska akademija, Beograd
cInstitut za javno zdravlje, Odsek za mikrobiologiju, Laboratorija za virusologiju i imunologiju, Kragujevac
dKlinički centar Kragujevac, Klinika za infektivne bolesti + Univerzitet u Kragujevcu, Fakultet medicinskih nauka
eUniverzitet u Kragujevcu, Fakultet medicinskih nauka
fKlinički centar Kragujevac, Klinika za ginekologiju i akušerstvo
gInfinity Family Medicine Clinic, Dubai, UAE
hInstitut za javno zdravlje, Odsek za mikrobiologiju, Laboratorija za virusologiju i imunologiju, Kragujevac + Univerzitet u Kragujevcu, Fakultet medicinskih nauka

e-adresa: dejan.baskic@gmail.com
Projekat:
Preklinička ispitivanja bioaktivnih supstanci (MPNTR - 41010)

Ključne reči: bakterijska vaginoza; real-time qPCR; Gardnerella; Atopobium
Sažetak
Uvod/Cilj Bakterijska vaginoza (BV) stanje je udruženo sa poremećajem odnosa laktobacila i anaerobnih bakterija u vagini u korist anaeroba. Teškoće u tumačenju mikroskopski klasifikovanih nalaza u dijagnostici BV zahtevaju molekularnu analizu bakterija prisutnih u vaginalnom sekretu. Cilj ovog rada je bio da real-time qPCR analizom uzoraka vaginalnog sekreta ispitamo u kom obimu su zastupljenost i količina anaeroba (Gardnerella vaginalis i Atopobium vaginae) u vezi sa mikroskopskim nalazima. Metode U studiju je uključeno 111 asimptomatskih trudnica starosti trudnoće 24-28 nedelja. Gram-preparati vaginalnih razmaza su kategorisani mikroskopski, a posle toga je sa gram-preparata izolovana DNK i izvedena reakcija identifikacije i kvantifikacije (real-time qPCR) G. vaginalis i A. vaginae. Rezultati Rezultati naše studije su pokazali da je 53,2% trudnica imalo normalan rezultat, dok je 20,7% i 26,1% imalo intermedijeran (IM) i BV rezultat. G. vaginalis i A. vaginae su bili češće prisutni u IM i BV grupi nego kod zdravih pacijentkinja, a i prosečan broj G. vaginalis i A. vaginae je bio značajno viši u BV i IM grupama nego u grupi sa normalnim nalazom (r = 0,000). Poredeći međusoban odnos G. vaginalis i A. vaginae, zastupljenost i broj G. vaginalis je u svim grupama bio značajno viši od zastupljenosti i broja A. vaginae. Zaključak Rezultati naše studije su pokazali da bi za razlikovanje normalnih od BV nalaza kvantifikacija bakterija mogla biti značajnija od same molekularne detekcije.
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O članku

jezik rada: engleski
vrsta rada: izvorni naučni članak
DOI: 10.2298/SARH170315206M
objavljen u SCIndeksu: 04.10.2018.

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