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2018, vol. 146, br. 7-8, str. 417-421
Molekularna dijagnoza bakterijske vaginoze - zastupljenost Gardnerella vaginalis i Atopobium vaginae kod trudnica
aKlinički centar Kragujevac
bVojnomedicinska akademija, Beograd
cInstitut za javno zdravlje, Odsek za mikrobiologiju, Laboratorija za virusologiju i imunologiju, Kragujevac
dKlinički centar Kragujevac, Klinika za infektivne bolesti + Univerzitet u Kragujevcu, Fakultet medicinskih nauka
eUniverzitet u Kragujevcu, Fakultet medicinskih nauka
fKlinički centar Kragujevac, Klinika za ginekologiju i akušerstvo
gInfinity Family Medicine Clinic, Dubai, UAE
hInstitut za javno zdravlje, Odsek za mikrobiologiju, Laboratorija za virusologiju i imunologiju, Kragujevac + Univerzitet u Kragujevcu, Fakultet medicinskih nauka

e-adresadejan.baskic@gmail.com
Projekat:
Preklinička ispitivanja bioaktivnih supstanci (MPNTR - 41010)

Ključne reči: bakterijska vaginoza; real-time qPCR; Gardnerella; Atopobium
Sažetak
Uvod/Cilj Bakterijska vaginoza (BV) stanje je udruženo sa poremećajem odnosa laktobacila i anaerobnih bakterija u vagini u korist anaeroba. Teškoće u tumačenju mikroskopski klasifikovanih nalaza u dijagnostici BV zahtevaju molekularnu analizu bakterija prisutnih u vaginalnom sekretu. Cilj ovog rada je bio da real-time qPCR analizom uzoraka vaginalnog sekreta ispitamo u kom obimu su zastupljenost i količina anaeroba (Gardnerella vaginalis i Atopobium vaginae) u vezi sa mikroskopskim nalazima. Metode U studiju je uključeno 111 asimptomatskih trudnica starosti trudnoće 24-28 nedelja. Gram-preparati vaginalnih razmaza su kategorisani mikroskopski, a posle toga je sa gram-preparata izolovana DNK i izvedena reakcija identifikacije i kvantifikacije (real-time qPCR) G. vaginalis i A. vaginae. Rezultati Rezultati naše studije su pokazali da je 53,2% trudnica imalo normalan rezultat, dok je 20,7% i 26,1% imalo intermedijeran (IM) i BV rezultat. G. vaginalis i A. vaginae su bili češće prisutni u IM i BV grupi nego kod zdravih pacijentkinja, a i prosečan broj G. vaginalis i A. vaginae je bio značajno viši u BV i IM grupama nego u grupi sa normalnim nalazom (r = 0,000). Poredeći međusoban odnos G. vaginalis i A. vaginae, zastupljenost i broj G. vaginalis je u svim grupama bio značajno viši od zastupljenosti i broja A. vaginae. Zaključak Rezultati naše studije su pokazali da bi za razlikovanje normalnih od BV nalaza kvantifikacija bakterija mogla biti značajnija od same molekularne detekcije.
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O članku

jezik rada: engleski
vrsta rada: izvorni naučni članak
DOI: 10.2298/SARH170315206M
objavljen u SCIndeksu: 04.10.2018.