Journal of Medical Biochemistry
kako citirati ovaj članak
podeli ovaj članak


  • citati u SCIndeksu: 0
  • citati u CrossRef-u:0
  • citati u Google Scholaru:[]
  • posete u poslednjih 30 dana:6
  • preuzimanja u poslednjih 30 dana:3


članak: 10 od 46  
Back povratak na rezultate
2018, vol. 37, br. 2, str. 163-171
Potential immune biomarkers in diagnosis and clinical management for systemic lupus erythematosus
(naslov ne postoji na srpskom)
aKlinički centar Univerziteta Sarajevo, Federacija BiH
bIn Medico, Department of Research & Development, Metlika, Slovenia
cUniverzitet u Sarajevu, Medicinski fakultet, Federacija BiH
dUniversity Josip Juraj Strossmayer of Osijek, Medical Faculty, Osijek, Croatia
eSt James University Hospital, Leeds, England

Ključne reči: SLE; biomarkers; T and B lymphocytes; cytokines; flow cytomet
(ne postoji na srpskom)
Background: There is still no reliable, specific biomarker for precision diagnosis and clinical monitoring of systemic lupus erythematosus. The aim of this study was to investigate the importance of the determination of immunofenotypic profiles (T, B lymphocytes and NK cells) and serum cytokine concentrations (IL-17 and IFN-alpha) as potential biomarkers for this disease. Methods: The study included 55 patients with SLE and 25 healthy controls. The proportion of T, B, NK cells were assessed in peripheral blood using flow cytometric assays while the serum cytokine concentration (IL-17 and IFNalpha) was determined by ELISA test. Results: ROC curve analysis showed good accuracy to distinguish between patients and healthy individuals for activated T cells (A U C = 0.798; p < 0.001), Treg (AUC = 0.651 ; p = 0.036), and memory B cells (A U C = 0.285; p = 0.002). We found statistically significant difference (p = 0.036) in the levels of serum IL-17 between patients with SLE (IL-17= 49.27 pg/mL) and controls (IL-17 = 28.64 pg/mL). Conclusions: Significant increase in the relative number of Treg lymphocytes, and decrease in memory B cells, as well as decrease level of IL-17, in SLE patients may be implicated in the pathogenesis of the disease. These parameters, as biomarkers, could distinguish SLE patients and no-SLE patients. Monitoring subpopulations of immune cells in peripheral blood using flow cytometry provides insight into abnormal T and B cell function in SLE. Progress in understanding the immunity at SLE, results in concrete benefits for the SLE patients, which include new clinical management and therapeutic strategies.
Abbas, A., Pillai, L.A.S. (2014) Activation of T lymphocytes. u: Abbas A K [ur.] Cellular and Molecular Immunology, Philadelphia Saunders, 199-212
Abu-Shakra, M., Novack, V. (2012) Mortality and multiple causes of death in systemic lupus erythematosus: Role of the death certificate. Journal of Rheumatology, 39(3): 458-460
Baecher-Allan, C., Brown, J.A., Freeman, G.J., Hafler, D.A. (2001) CD4+CD25high regulatory cells in human peripheral blood. Journal of Immunology, 167(3): 1245-1253
Castro, C., Gourley, M. (2010) Diagnostic testing and interpretation of tests for autoimmunity. Journal of Allergy and Clinical Immunology, 125(2): S238-S247
Choi, J., Kim, S.T., Craft, J. (2012) The pathogenesis of systemic lupus erythematosus: An update. Current Opinion in Immunology, 24(6): 651-657
Collinson, P. (2017) Laboratory medicine is faced with the evolution of medical practice. Journal of Medical Biochemistry, vol. 36, br. 3, str. 211-215
Comte, D., Karampetsou, M.P., Tsokos, G.C. (2015) T cells as a therapeutic target in SLE. Lupus, 24(4-5): 351-363
Cripsin, J.C., Tsokos, G.C. (2013) T Cells. u: Hahn B.H.; Wallace D.J. [ur.] Dubois' lupus erythematosus and related syndromes, Philidelphia Saunders, 96-104
Crispin, J., Liossis, S., Kis-Toth, K., et al. (2011) Pathogenesis of human systemic lupus erythematosus: Recent advances. Trends Mol Med, 16: 47-57
Crispin, J.C., Martinez, A., Alcocer-Varela, J. (2003) Quantification of regulatory T cells in patients with systemic lupus erythematosus. Journal of Autoimmunity, 21(3): 273-276
D'cruz, D.P., Khamashta, M.A., Hughes, G.R.V. (2007) Systemic lupus erythematosus. Lancet, 369(9561): 587-596
Deng, Y., Tsao, B.P. (2014) Advances in lupus genetics and epigenetics. Curr Opin Rheumatol, 26: 482-92
Dolff, S., Quandt, D., Wilde, B., Feldkamp, T., Hua, F., Cai, X., Specker, C., Kribben, A., Kallenberg, C.G., Witzke, O. (2010) Increased expression of costimulatory markers CD134 and CD80 on interleukin-17 producing T cells in patients with systemic lupus erythematosus. Arthritis Research & Therapy, 12(4): R150-R150
Formiga, F., Moga, I., Pac, M., Mitjavila, F., Rivera, A., Pujol, R. (1999) Mild presentation of systemic lupus erythematosus in elderly patients assessed by SLEDAI. SLE Disease Activity Index. Lupus, 8(6): 462-5
Hochberg, M.C. (1997) Updating the American college of rheumatology revised criteria for the classification of systemic lupus erythematosus. Arthritis & Rheumatism, 40(9): 1725-1725
Hoffman, R. (2004) T cells in the pathogenesis of systemic lupus erythematosus. Clinical Immunology, 113(1): 4-13
Iikuni, N., Lourenço, E.V., Hahn, B.H., La, C.A. (2009) Cutting edge: Regulatory T cells directly suppress B cells in systemic lupus erythematosus. Journal of Immunology, 183(3): 1518-1522
Lee, J.Y., Park, J.K., Lee, E.Y., Lee, E.B., Song, Y.W. (2016) Circulating exosomes from patients with systemic lupus erythematosus induce a proinflammatory immune response. Arthritis Research & Therapy, 18(1): 264-264
Liu, C.C., Kao, A., Manzi, S., Ahearn, J. (2013) Biomarkers in systemic lupus erythematosus: Challenges and prospects for the future. Ther Adv Musculoskelet Dis, 5: 210-33
Lloyd, P., Doaty, S., Hahn, B.H. (2016) Aetiopathogenesis of systemic lupus erythematosus. u: Gordon C.; Isenberg D. [ur.] Systemic Lupus Erythematosus, Oxford University Press, 7-23
Madhok, R., Wu, O. (2007) Systemic lupus erythematosus. American Family Physician, 76: 1351-3
Mak, A., Kow, N.Y. (2014) The pathology of T cells in systemic lupus erythematosus. Journal of Immunology Research, 2014: 1-8
Petri, M., Orbai, A.M., Alarc, N. (2012) Derivation and validation of the systemic lupus international collaborating clinics classification criteria for systemic lupus erythematosus. Arthritis Rheum, 64: 2677-86
Rahman, A., Isenberg, D.A. (2008) Systemic Lupus Erythematosus. New England Journal of Medicine, 358(9): 929-939
Sestak, A.L., Furnrohr, B.G., Harley, J.B., Merrill, J.T., Namjou, B. (2011) The genetics of systemic lupus erythematosus and implications for targeted therapy. Annals of the Rheumatic Diseases, 70(Suppl 1): i37-i43
Suarez, A., Lopez, P., Gomez, J., Gutierrez, C. (2006) Enrichment of CD4+ CD25high T cell population in patients with systemic lupus erythematosus treated with glucocorticoids. Annals of the Rheumatic Diseases, 65(11): 1512-1517
Tomioka, R., Tani, K., Sato, K., Suzuka, C., Toyoda, Y., Kishi, J., Sone, S. (2008) Observations on the occurrence of exacerbations in clinical course of systemic lupus erythematosus. Journal of Medical Investigation, 55(1,2): 112-119
Valencia, X., Yarboro, C., Illei, G., Lipsky, P.E. (2007) Deficient CD4+CD25high T regulatory cell function in patients with active systemic lupus erythematosus. Journal of Immunology, 178(4): 2579-2588
Venigalla, R.K., Tretter, T., Krienke, S., et al. (2008) Reduced C D 4+ ,C D 25-T cell sensitivity to the suppressive function of C D 4 + , CD25high, CD127-/low regulatory T cells in patients with active systemic lupus erythematosus. Arthritis Rheum, 2120-30
Viallard, J.F., Bloch-Michel, C., Neau-Cransac, M., Taupin, J.L., Garrigue, S., Miossec, V., Mercie, P., Pellegrin, J.L., Moreau, J.F. (2001) HLA-DR expression on lymphocyte subsets as a marker of disease activity in patients with systemic lupus erythematosus. Clinical and Experimental Immunology, 125(3): 485-491
Wong, C.K., Lit, L.C.W., Tam, L.S., Li, E.K.M., Wong, P.T.Y., Lam, C.W.K. (2008) Hyperproduction of IL-23 and IL-17 in patients with systemic lupus erythematosus: Implications for Th17-mediated inflammation in auto-immunity. Clinical Immunology, 127(3): 385-393
Yan, B., Ye, S., Chen, G., Kuang, M., Shen, N., Chen, S. (2008) Dysfunctional CD4+,CD25+ regulatory T cells in untreated active systemic lupus erythematosus secondary to interferon-a-producing antigen-presenting cells. Arthritis & Rheumatism, 58(3): 801-812
Yang, J., Chu, Y., Yang, X., Gao, D., Zhu, L., Yang, X., Wan, L., Li, M. (2009) Th17 and natural Treg cell population dynamics in systemic lupus erythematosus. Arthritis & Rheumatism, 60(5): 1472-1483
Yates, J., Whittington, A., Mitchell, P., Lechler, R.I., Lightstone, L., Lombardi, G. (2008) Natural regulatory T cells: Number and function are normal in the majority of patients with lupus nephritis. Clinical & Experimental Immunology, 153(1): 44-55