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Veterinarski glasnik
2020, vol. 74, iss. 1, pp. 18-33
article language: English
document type: Original Paper
published on: 26/07/2020
doi: 10.2298/VETGL190414019M
Platelet-poor plasma of athletes is a potent inducer of Myogenic differentiation of C2C12 myoblasts
aRed Star Weightlifting Club, Belgrade + University of Belgrade, Institute for Medical Research
bUniversity of Belgrade, Institute for Medical Research

e-mail: vesnai@imi.bg.ac.rs

Project

This work was supported by grants from the Ministry of Youth and Sport, Republic of Serbia (Contract No. 450-451/02-012713/1/2011-03)
Regenerative and modulatory potential of adult stem cells (MESTD - 175062)

Abstract

Introduction. Blood products, i.e. platelet rich plasma (PRP), leukocyte-poor plasma (PRP) and platelet poor plasma (PPP), have previously been used to improve muscle regeneration. In this study, six months' frozen-stored PPP of individuals who practiced different types of physical exercise was analysed; it could steer mouse C2C12 myoblast cells towards proliferation, migration and myogenic differentiation, and it could affect the morphology/shape of myotubes. Materials and Methods. PPP of male Olympic weightlifters, football players and professional folk dancers, aged 15-19, was collected 12 h post-training and stored for 6 months at -20°C. C2C12 cell proliferation was assessed by MTT test, motility by scratch assay, myogenic differentiation by myotube formation and gelatinase activity by gel-zymography. Results and Conclusions. PPP induced proliferation and migration of C2C12 cells. Proliferative capacity was as follows: weightlifters > dancers > football players; mean migratory capacity was: weightlifters = dancers > football players. PPP induced formation of myotubes; significant inter-individual variations were detected: PPP from weightlifters induced formation of round myotubes, and PPP from football players and dancers induced formation of elongated myotubes. The mean myotube area was as follows: football players > dancers > weightlifters. PPP gelatinolytic activity was observed; it was negatively correlated with C2C12 myoblast proliferation. These results provide general but distinct evidence that PPP of individuals practicing certain types of exercise can specifically modify myoblast morphology/function. This is significant for explaining physiological responses and adaptations to exercise. In conclusion, longterm, frozen-stored PPP preserves its potential to modify myoblast morphology and function.

Keywords

C2C12 myoblasts; Exercise; Myogenic differentiation; Myotube morphology

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