Kragujevac Journal of Science
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2021, br. 43, str. 23-34
Flavonoids from aerial part of Algerian Ajuga iva (L.) schreb.: The HPLC-UV analysis and Antioxidant capacity
(naslov ne postoji na srpskom)
University of Mascara, Faculty of Science of Nature and Life, Mascara, Algeria

Ključne reči: Antioxidant effect; flavonoids; polyphenols; HPLC
(ne postoji na srpskom)
The study deals with the evaluation of the antioxidant capacity of extracts from the aerial part of Algerian Ajuga iva. Extraction of flavonoids was carried out by 85% of methanol, then the crude extract was successively separated with ethyl acetate, butanol, and water. The in vitro antioxidant activity was assessed by 1,1-diphenyl-2-picrylhydrazyl, reducing power, and thiobarbituric acid reacting substances assays. Extracts are subject to HPLC-UV analysis. The average total phenol contents of extracts vary between 3.87 ± 0.17 and 149.74 ± 3.94 (gallic acid equivalent per gram of dry extract). Furthermore, tested extracts exhibited a broad range of flavonoid contents varying from 1.54 ± 0.09 to 41.18 ± 1.03 (catechin equivalent per gram of dry extract). Butanol and ethyl acetate fractions displayed the highest antioxidant activity. A good correlation between the phenolic and flavonoid contents and the antioxidant activity was observed. Rutin, caffeic acid, quercetin, p-coumaric acid, luteolin, and cinnamic acid were present in the extracts. The plant could be a potential source of antioxidant agents.
Adjadj, M. (2009) Propriétés antioxydantes et activité inhibitrice de la xanthine oxydase des extraits de la plante medicinal Ajuga iva. u: Mémoire de magister en toxicologie cellulaire et moleculaire, Constantine, Algérie: Uniersité de Mentouri, 86: pp. 51-52
Anokwuru, C.P., Anyasor, G.N., Ajibaye, O., Fakoya, O., Okebugwu, P. (2011) Effect of extraction solvents on phenolic, flavonoid and antioxidant activities of three Nigerian medicinal plants. Nature and Science, 9 (7): 53-61
Ardestani, A., Yazdanparast, R. (2007) Inhibitory effects of ethyl acetate extract of Teucrium polium on in vitro protein glicoxidation. Food and Chemical Toxicology, 45: 2402-2411
Ayari, B., Riah, L., Ziadi, S., Chograni, H., Mliki, A. (2013) Evaluation of antioxidant and antimicrobial activities of Tunisian Ajuga iva L. essential oils. Revue f. S. B, XI: 203-210
Baghiani, A., Boumerfeg, S., Adjadj, M., Ameni, D., Djermouni, M., Khelifitouhami, F., Charef, N., Khennouf, S., Arrar, L. (2011) Antioxidants, free radicals scavenging and xanthine oxidase inhibitory potentials of Ajuga iva L. extracts. Free Radicals and Antioxidants, 1 (4): 21-30
Batanouny, K.H., Abou, T.S., Shabana, M., Soliman, F. (1999) Wild medicinal plants in Egypt. Egypt: Academy of Scientific Research and Technology
Bouderbala, S., Lamri-Senhadji, M., Prost, J., Lacaille-Dubois, M.A., Bouchenak, M. (2008) Changes in antioxidant defense status in hypercholesterolemic rats treated with Ajuga iva. Phytomedicine, 15(6-7): 453-461
Boudjelal, A., Siracusa, L., Henchiri, C., Sarri, M., Abderrahim, B., Baali, F., Ruberto, G. (2015) Antidiabetic Effects of Aqueous Infusions of Artemisia herba-alba and Ajuga iva in Alloxan-Induced Diabetic Rats. Planta Medica, 81(09): 696-704
Bozin, B., Mimica-Dukic, N., Samajilik, I., Jovin, E. (2007) Antimicrobial and antioxidant properties of Rosmary and sage essential oils. Journal of Agricultural and Food Chemistry, 55: 7878-7885
Cai, Q., Rahn, R.O., Zhang, R. (1997) Dietary flavonoids, quercetin, luteolin and genistein, reduce oxidative DNA damage and lipid peroxidation and quench free radicals. Cancer Letters, 119(1): 99-107
Cao, G., Sofic, E., Prior, R.L. (1997) Antioxidant and Prooxidant Behavior of Flavonoids: Structure-Activity Relationships. Free Radical Biology and Medicine, 22(5): 749-760
Chabane, D., Saidi, F., Rouibi, A., Azine, K. (2013) Activité hypoglycémique de l'extrait aqueux d'Ajuga iva L. schreber chez les rats diabétiques induite par l'alloxane. Afrique Science, 9(1): 120-127
Chang, M.Y., Liu, C.M., Shieh, D.E., Chen, C.Y. (2017) Evaluation and analysis of phytochemical antioxidant capacity. Biomedical Research, 28(14): 6431-6434
Crozier, A., Clifford, M.N., Ashihara, H. (2008) Plant secondary metabolites: Occurrence, structure and role in the human diet. Blackwell Publishing Ltd
Degraft-Johnson, J., Kolodziejczyk, K., Krol, M., Nowak, P., Krol, B., Nowak, D. (2007) Ferric-reducing ability power of selected plant polyphenols and their metabolites: Implications for clinical studies on the Antioxidant effects of fruits and vegetable consumption. Basic & Clinical Pharmacology & Toxicology, 100(5): 345-352
Ebrahimi, N.S., Hadian, J., Mirjalili, M.H., Sonboli, A., Yousefzadi, M. (2008) Essential oil composition and antimicrobial activity of Thymus caramanicus at different phonological stages. Food Chemistry, 110: 927-931
El-Hilaly, J., Lyoussi, B., Wibo, M., Morel, N. (2004) Vasorelaxant effect of the aqueous extract of Ajuga iva in rat aorta. Journal of Ethnopharmacology, 93(1): 69-74
Farahmand, S.K., Samini, F., Samini, M., Samarghandian, S. (2013) Safranal ameliorates antioxidant enzymes and suppresses lipid peroxidation and nitric oxide formation in aged male rat liver. Biogerontology, 14(1): 63-71
Favier, A. (2003) Le stress oxydant: Intérêt conceptuel et expérimental dans la compréhension des mécanismes des maladies et potentiel thérapeutique. L'actualité chimique, 108-115
Halimi, A.K. (2004) Les plantes médicinales en Algérie. Alger: BERTI, 1ère Ed, pp: 156-157
Heinonen, I., Lehtonen, P.J., Hopia, A.I. (1998) Antioxidant activity of berry and fruit wines and liquors. Journal of Agricultural and Food Chemistry, 48: 25-31
Israili, Z.H., Lyoussi, B. (2009) Ethnopharmacology of theplants of genus Ajuga. Pak J Pharm Sci, 22(4): 425-462
Janssen, A.M., Scheffer, J.J.C., Svendsen, A.B. (1976) Antimicrobial Activity of Essential Oils: A 1976-1986 Literature Review. Aspects of the Test Methods. Planta Medica, 53(05): 395-398
Karagözler, A.A., Erdağ, B., Emek, Y.Ç., Uygun, D.A. (2008) Antioxidant activity and proline content of leaf extracts from Dorystoechas hastata. Food Chemistry, 111(2): 400-407
Khatteli, A., Benabderrahim, M.A., Triki, T., Guasmi, F. (2020) Aroma volatiles, phenolic profile and hypoglycaemic activity of Ajuga iva L. Food Bioscience, 36: 100578-100578
Kouri, G., Tsimogiannis, D., Bardouki, H., Oreopoulou, V. (2007) Extraction and analysis of antioxidant components from Origanum dictamnus. Innovative Food Science & Emerging Technologies, 8(2): 155-162
Makni, M., Haddar, A., Kriaa, W., Zeghal, N. (2013) Antioxidant, Free Radical Scavenging, and Antimicrobial Activities of Ajuga iva Leaf Extracts. International Journal of Food Properties, 16(4): 756-765
Merghem, R., Jay, M., Viricel, M.R., Bayet, C., Voirin, B. (1995) Five 8-C-benzylated flavonoids from Thymus hirtus (Labiateae). Phytochemistry, 38(3): 637-640
Miliauskas, G., Venskutonis, P.R., van Beek, T.A. (2004) Screening of radical scavenging activity of some medicinal and aromatic plant extracts. Food Chemistry, 85(2): 231-237
Molyneux, P. (2004) The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin Journal of Science and Technology, 26: 211-219
Oyaizu, M. (1986) Studies on products of browning reaction: Antioxidative activities of products of browning reaction prepared from glucosamine. Japanese Journal of Nutrition and Dietetics, 44(6): 307-315
Ozturk, M., Aydogmus-Ozturk, F., Duru, M.E., Topçu, G. (2007) Antioxidant activity of stem and root extracts of Rhubarb (Rheum ribes): An edible medicinal plant. Food Chemistry, 103: 623-630
Ratty, A.K., Das, N.P. (1988) Effects of flavonoids on nonenzymatic lipid peroxidation: Structure-activity relationship. Biochemical Medicine and Metabolic Biology, 39(1): 69-79
Robbins, R. (2003) Phenolic acids in foods: An overview of analytical methodology. Journal of Agricultural and Food Chemistry, 51(10): 2866-2887
Salucci, M., Stivala, L.A., Maiani, G., Bugianesi, R., Vannini, V. (2002) Flavonoids uptake and their effect on cell cycle of human colon adenocarcinoma cells. British Journal of Cancer, 86(10): 1645-1651
Shahidi, F.P.K.J., Janitha, P.K., Wanasundara, P.D. (1992) Phenolic antioxidants. Critical Reviews in Food Science and Nutrition, 32(1): 67-103
Shimada, K., Fujikawa, K., Yahara, K., Nakamura, T. (1992) Antioxidative properties of xanthan on the autoxidation of soybean oil in cyclodextrin emulsion. Journal of Agricultural and Food Chemistry, 40(6): 945-948
Taleb-Senouci, D., Ghomari, H., Krouf, D., Bouderbala, S., Prost, J., Lacaille-Dubois, M.A., Bouchenak, M. (2009) Antioxidant effect of Ajuga iva aqueous extract in streptozotocin-induced diabetic rats. Phytomedicine, 16(6-7): 623-631
Tripathi, Y.B., Singh, A.V. (2001) Effect of Semecarpus anacardium nuts on lipid peroxidation. Indian Journal of Experimental Biology, 39: 798-801
Vamanu, E., Nita, S. (2013) Antioxidant capacity and the correlation with major phenolic compounds, anthocyanin, and tocopherol content in various extracts from the wild edible Boletus edulis mushroom. BioMed Research International, 1-11
van Acker, S.A.B.E., van Balen, G.P., van den Berg, D., Bast, A., van der Vijgh, W.J.F. (1998) Influence of iron chelation on the antioxidant activity of flavonoids. Biochemical Pharmacology, 56(8): 935-943
Velioglu, Y.S., Mazza, G., Gao, L., Oomah, B.D. (1998) Antioxidant activity and total phenolics in selected fruits, vegetables, and grain products. Journal of Agricultural and Food Chemistry, 46(10): 4113-4117
Wojdylo, A., Oszmianski, J., Czemerys, R. (2007) Antioxidant activity and phenolic compounds in 32 selected herbs. Food Chemistry, 105(3): 940-949
Wong, S.P., Leong, L.P., Williamkoh, J. (2006) Antioxidant activities of aqueous extracts of selected plants. Food Chemistry, 99(4): 775-783
Yang, J., Guo, J., Yuan, J. (2008) In vitro antioxidant properties of rutin. LWT - Food Science and Technology, 41(6): 1060-1066
Zafar, H.I., Badiàa, L. (2009) Ethnopharmacology of the plants of the genus Ajuga. Pakistan journal of pharmaceutical sciences, 22(4): 425-462
Zerroug, M.M., Zouaghi, M., Boumerfeg, S., Baghiani, A., Nicklin, J., Arrar, L. (2011) Antibacterial activity of extracts of Ajuga iva and Teucrium polium. Advances in Environmental Biology, 5(2): 491-495
Zhang, M., Swarts, S.G., Yin, L., Liu, C., Tian, Y., Cao, Y., Swarts, M., Yang, S., Zhang, S.B., Zhang, K., Ju, S., Olek, D.J., Schwartz, L., Keng, P.C., Howell, R., Zhang, L., Okunieff, P. (2011) Antioxidant Properties of Quercetin. Advances in Experimental Medicine and Biology, 701: 283-289

O članku

jezik rada: engleski
vrsta rada: izvorni naučni članak
DOI: 10.5937/KgJSci2143023B
primljen: 09.01.2021.
prihvaćen: 05.05.2021.
objavljen u SCIndeksu: 02.07.2021.
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