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2019, vol. 8, br. 1, str. 10-18
Postupak ekstrakcije ukupnih flavonoida iz cveta nevena (Calendulae officinalis L.)
Univerzitet u Nišu, Tehnološki fakultet, Leskovac

e-adresavana.savic@yahoo.com
Projekat:
Biljni i sintetički bioaktivni proizvodi novije generacije (MPNTR - 34012)

Ključne reči: ultrazvučna ekstrakcija; flavonoidi; Calendulae officinalis; Box-Behnken
Sažetak
Neven (Calendula officinalis L.) sadrži različita bioaktivna jedinjenja, kao što su: flavonoidi, tanini, saponozidi, karotenoidi, triterpenski alkoholi, polisaharidi, aminokiseline i etarsko ulje. Ekstrakt cveta nevena blagotvorno deluje na kožu usled prisustva flavonoida koji imaju antiinflamatorno, adstrigentno, antifungalno i antiseptično dejstvo. Cilj rada bio je da se primeni ultrazvučna ekstrakcija kao savremena tehnika i da se ispita uticaj vremena ekstrakcije (5-35 min), koncentracije etanola (20-80%, v/v) i temperatura ekstrakcije (30-70 °C) na sadržaj ukupnih flavonoida cveta nevena pri odnosu rastvarača i biljne sirovine od 20 cm3 g-1. Sadržaj ukupnih flavonoida ekstrakata određen je spektrofotometrijski sa aluminijum(III) hloridom. Modelovanje ultrazvučne ekstrakcije izvršeno je primenom Box-Behnken-ovog dizajna. Tako dobijeni optimalni uslovi bili su: vreme ekstrakcije od 29 min, 39,6% (v/v) etanol i temperature ekstrakcije od 64,2 °C. Eksperimentalna vrednost (220,2 mg 100 g-1s.m.) sadržaja ukupnih flavonoida pri optimalnim uslovima bila je u dobroj saglasnosti sa predviđenom vrednošću (221,5 mg 100 g-1s.m.). Prema rezultatima statističke analize, predložena polinomna jednačina drugog reda može se upotrebiti da opiše ekstrakciju flavonoida i da predvidi sadržaj ukupnih flavonoida. Postupak ekstrakcije može se prihvatiti sa stanovišta farmaceutske primene usled primene etanola kao predstavnika zelenih rastvarača. Vreme ekstrakcije bilo je kraće u poređenju sa drugim konvencionalnim tehnikama ekstrakcije.
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O članku

jezik rada: engleski
vrsta rada: izvorni naučni članak
DOI: 10.5937/SavTeh1901010Z
objavljen u SCIndeksu: 08.07.2019.