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2006, vol. 60, br. 3-4, str. 87-91
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Određivanje hiperozida u cvetu i listu gloga (Crataegus oxycantha i Crataegus monogyna) primenom metode spektrofotometrije
The determination of hyperoside in the flower and leaf of hawthorn (Crataegus oxycantha and Crataegus monogyna) by the spectrophotometric method
FHI Zdravlje Actavis Company - Centar za istraživanje i razvoj, Leskovac
Sažetak
Cvet i list gloga sadrže veliki broj aktivnih komponenata, koje imaju široku primenu u proizvodnji farmacetskih preparata iz grupe kardiotonika antihipertonika, antihipotonika, arteriosklerotika antiaritmika i antianemika. Antihipertonični efekat gloga potiče od hiperozida. Zbog lekovitog delovanja hiperozida i drugih flavonoida iz gloga njegovo određivanje ima veliki značaj za ocenu kvaliteta ekstrakta i preparata. Ekstrakcija flavonoida iz lista i cveta gloga (C. oxycantha i C. monogyna) vršena je sa 40 %-tnim i 70 %-tnim etanolom, postupkom perkolacije, a suvi ekstrakt je dobijen prečišćavanjem tečnog ekstrakta gloga sa etilacetatom i etanolom. Za odredivanje sadrzaja ukupnih flavonoida obračunatih kao hiperozid, primenjen je spektrofotometrijski metod, zasnovan na merenju apsorbancija na 425 nm.
Abstract
The flower and leaf of hawthorn contains many active components, used in the production of pharmaceutical preparations for cardiotonic, anti-hypertonic anti-hypotonic, artherosclerotic, anti-arithmic and anti-anemic groups. According to the ROTTE list (1996), 159 of 1800 pharmaceutical preparations contain components from hawthorn. Due to the remedial action of hyperoside and other flavonoids from hawthorn, their determination is important for the standardization of extract and preparation quality. The extraction of the flavonoids from a mixture of hawthorn (C. oxycantha and C. monogyna) flower and leaf was carried out by percolation with 40% and 70% (v/v) ethanol. In order to determine the content of total flavonoids calculated as hyperoside spectrophotometric method with aluminium(lll) chloride as a colour reagent at 425 nm was applied. The total flavonoid content in the herbal material mixture was 0.483%, in 40% and 70% (v/v) ethanolic liquid extract it was 138 mg/100 cm3 and 157 mg/100 cm, respectively. The extraction degree of total flavonoids with 40% and 70% (v/v) ethanolic solutions was 57.14% and 65.01% respectively. The yield of total flavonoids in the dry extract was 63% in regard to the herbal mixure content.
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